Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway
Mesenchymal stem cells (MSCs) are multipotent cells that can skew the balance of M1/M2 macrophage polarization towards the M2 phenotype via their paracrine effects, thereby promoting anatomical and functional recovery after many inflammatory diseases induced by macrophages. However, the underlying m...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/5181241 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550800427057152 |
|---|---|
| author | Qi-Ming Pang Rui Yang Meng Zhang Wang-Hui Zou Nan-Nan Qian Qi-Jing Xu Hui Chen Jia-Chen Peng Xiao-Ping Luo Qian Zhang Tao Zhang |
| author_facet | Qi-Ming Pang Rui Yang Meng Zhang Wang-Hui Zou Nan-Nan Qian Qi-Jing Xu Hui Chen Jia-Chen Peng Xiao-Ping Luo Qian Zhang Tao Zhang |
| author_sort | Qi-Ming Pang |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are multipotent cells that can skew the balance of M1/M2 macrophage polarization towards the M2 phenotype via their paracrine effects, thereby promoting anatomical and functional recovery after many inflammatory diseases induced by macrophages. However, the underlying mechanism is still poorly understood. This study focused on the IL-10/STAT3 pathway and investigated whether IL-10 secreted by PBMSCs could mediate M2 polarization through the activation of this pathway. In this study, a Transwell system was used for coculturing macrophages and PBMSCs. ELISA and RT-qPCR analysis found that PBMSCs and their conditioned media (P-CM) significantly induced the expression of IL-10, while significantly inhibiting the expression of IL-1β and TNF-α; moreover, this effect could be reversed by adding Ab9969 (an IL-10 neutralizing antibody) and Stattic (a STAT3 inhibitor). Furthermore, western blotting and immunofluorescence assays demonstrated that JAK1/STAT3 signaling was significantly upregulated in macrophages cocultured with PBMSCs or P-CM, accompanied by an increase in the M2 biomarker CD206 and a decrease in the M1 biomarker CD86. This effect could also be reversed by blocking the IL-10/STAT3 pathway with Ab9969 and Stattic. In summary, PBMSCs could mediate the polarization of M2 macrophages by activating the IL-10/STAT3 pathway. |
| format | Article |
| id | doaj-art-b0a3937e7d95489db00ba3ec9d75fa6b |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b0a3937e7d95489db00ba3ec9d75fa6b2025-02-03T06:05:48ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/5181241Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 PathwayQi-Ming Pang0Rui Yang1Meng Zhang2Wang-Hui Zou3Nan-Nan Qian4Qi-Jing Xu5Hui Chen6Jia-Chen Peng7Xiao-Ping Luo8Qian Zhang9Tao Zhang10Key Laboratory of Cell Engineering of Guizhou Province and Regenerative Medicine CentreKey Laboratory of Cell Engineering of Guizhou Province and Regenerative Medicine CentreKey Laboratory of Cell Engineering of Guizhou Province and Regenerative Medicine CentreKey Laboratory of Cell Engineering of Guizhou Province and Regenerative Medicine CentreKey Laboratory of Cell Engineering of Guizhou Province and Regenerative Medicine CentreDepartment of Human AnatomyKey Laboratory of Cell Engineering of Guizhou Province and Regenerative Medicine CentreDepartment of OrthopedicsKey Laboratory of Cell Engineering of Guizhou Province and Regenerative Medicine CentreDepartment of Human AnatomyKey Laboratory of Cell Engineering of Guizhou Province and Regenerative Medicine CentreMesenchymal stem cells (MSCs) are multipotent cells that can skew the balance of M1/M2 macrophage polarization towards the M2 phenotype via their paracrine effects, thereby promoting anatomical and functional recovery after many inflammatory diseases induced by macrophages. However, the underlying mechanism is still poorly understood. This study focused on the IL-10/STAT3 pathway and investigated whether IL-10 secreted by PBMSCs could mediate M2 polarization through the activation of this pathway. In this study, a Transwell system was used for coculturing macrophages and PBMSCs. ELISA and RT-qPCR analysis found that PBMSCs and their conditioned media (P-CM) significantly induced the expression of IL-10, while significantly inhibiting the expression of IL-1β and TNF-α; moreover, this effect could be reversed by adding Ab9969 (an IL-10 neutralizing antibody) and Stattic (a STAT3 inhibitor). Furthermore, western blotting and immunofluorescence assays demonstrated that JAK1/STAT3 signaling was significantly upregulated in macrophages cocultured with PBMSCs or P-CM, accompanied by an increase in the M2 biomarker CD206 and a decrease in the M1 biomarker CD86. This effect could also be reversed by blocking the IL-10/STAT3 pathway with Ab9969 and Stattic. In summary, PBMSCs could mediate the polarization of M2 macrophages by activating the IL-10/STAT3 pathway.http://dx.doi.org/10.1155/2022/5181241 |
| spellingShingle | Qi-Ming Pang Rui Yang Meng Zhang Wang-Hui Zou Nan-Nan Qian Qi-Jing Xu Hui Chen Jia-Chen Peng Xiao-Ping Luo Qian Zhang Tao Zhang Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway Stem Cells International |
| title | Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway |
| title_full | Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway |
| title_fullStr | Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway |
| title_full_unstemmed | Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway |
| title_short | Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway |
| title_sort | peripheral blood derived mesenchymal stem cells modulate macrophage plasticity through the il 10 stat3 pathway |
| url | http://dx.doi.org/10.1155/2022/5181241 |
| work_keys_str_mv | AT qimingpang peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway AT ruiyang peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway AT mengzhang peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway AT wanghuizou peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway AT nannanqian peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway AT qijingxu peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway AT huichen peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway AT jiachenpeng peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway AT xiaopingluo peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway AT qianzhang peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway AT taozhang peripheralbloodderivedmesenchymalstemcellsmodulatemacrophageplasticitythroughtheil10stat3pathway |