Exploring the Antioxidant and Neuroprotective Potential of Muscari armeniacum: Phenolic Profiling and Enzyme Inhibition
This study aimed to investigate the antioxidant and enzyme inhibitory properties of Muscari armeniacum, a plant native to Türkiye, which is known for its bioactive compound content. Ethanol extracts of Muscari armeniacum were prepared and analyzed using a variety of bioanalytical methods to assess i...
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Turkish Science and Technology Publishing (TURSTEP)
2025-01-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | https://agrifoodscience.com/index.php/TURJAF/article/view/7355 |
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| author | Fevzi Topal Firat Yılmaz Meryem Topal Ahmed Menevşeoğlu |
| author_facet | Fevzi Topal Firat Yılmaz Meryem Topal Ahmed Menevşeoğlu |
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| collection | DOAJ |
| description | This study aimed to investigate the antioxidant and enzyme inhibitory properties of Muscari armeniacum, a plant native to Türkiye, which is known for its bioactive compound content. Ethanol extracts of Muscari armeniacum were prepared and analyzed using a variety of bioanalytical methods to assess its potential health-promoting properties. The antioxidant capacity was evaluated through Fe³⁺-Fe²⁺ reduction capacity, CUPRAC, DPPH, and ABTS radical scavenging activities. Additionally, the total phenolic and flavonoid contents of the extracts were determined, as these compounds are critical indicators of antioxidant potential. The antioxidant activity of Muscari armeniacum was compared with five standard antioxidants commonly used for benchmarking: BHA, α-tocopherol, BHT, trolox, and ascorbic acid. The extract demonstrated a high flavonoid content (23.24 µg QE/mg extract) and a significant amount of phenolics (14.38 µg GAE/mg extract), with the flavonoid content being particularly noteworthy. In addition to its antioxidant properties, the enzymatic activity assays revealed that the extract significantly inhibited acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes, which are associated with neurodegenerative diseases such as Alzheimer’s. The IC₅₀ values were determined to be 54.14 mg/mL (R² = 0.9695) for AChE and 58.73 mg/mL (R² = 0.9609) for BChE, indicating moderate enzyme inhibition. These results suggest that Muscari armeniacum could serve as a valuable source of bioactive compounds with significant antioxidant and enzyme inhibitory activities. Its potential applications in the treatment of oxidative stress-related diseases and neurodegenerative disorders make it a promising candidate for further pharmacological studies. This research underscores the importance of exploring native plants for their medicinal and therapeutic potential, contributing to the development of alternative treatments for challenging health conditions. |
| format | Article |
| id | doaj-art-b07fc67cbcdd473c8c8eaba2d0e4e067 |
| institution | Kabale University |
| issn | 2148-127X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-b07fc67cbcdd473c8c8eaba2d0e4e0672025-02-06T13:44:57ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2025-01-0113120420910.24925/turjaf.v13i1.204-209.73556056Exploring the Antioxidant and Neuroprotective Potential of Muscari armeniacum: Phenolic Profiling and Enzyme InhibitionFevzi Topal0https://orcid.org/0000-0002-2443-2372Firat Yılmaz1https://orcid.org/0000-0003-3633-0012Meryem Topal2https://orcid.org/0000-0002-2107-8603Ahmed Menevşeoğlu3https://orcid.org/0000-0003-2454-7898Gumushane University, Faculty of Engineering and Natural Sciences, Department of Food Engineering, 29100 Gumushane, TürkiyeGumushane University, Faculty of Engineering and Natural Sciences, Department of Food Engineering, 29100 Gumushane, TürkiyeGumushane University, Vocational School of Health Services, 29100 Gumushane, TürkiyeAgri Ibrahim Cecen University, School of Tourism and Hotel Management, Department of Gastronomy and Culinary Arts, 04100 Agri, TürkiyeThis study aimed to investigate the antioxidant and enzyme inhibitory properties of Muscari armeniacum, a plant native to Türkiye, which is known for its bioactive compound content. Ethanol extracts of Muscari armeniacum were prepared and analyzed using a variety of bioanalytical methods to assess its potential health-promoting properties. The antioxidant capacity was evaluated through Fe³⁺-Fe²⁺ reduction capacity, CUPRAC, DPPH, and ABTS radical scavenging activities. Additionally, the total phenolic and flavonoid contents of the extracts were determined, as these compounds are critical indicators of antioxidant potential. The antioxidant activity of Muscari armeniacum was compared with five standard antioxidants commonly used for benchmarking: BHA, α-tocopherol, BHT, trolox, and ascorbic acid. The extract demonstrated a high flavonoid content (23.24 µg QE/mg extract) and a significant amount of phenolics (14.38 µg GAE/mg extract), with the flavonoid content being particularly noteworthy. In addition to its antioxidant properties, the enzymatic activity assays revealed that the extract significantly inhibited acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes, which are associated with neurodegenerative diseases such as Alzheimer’s. The IC₅₀ values were determined to be 54.14 mg/mL (R² = 0.9695) for AChE and 58.73 mg/mL (R² = 0.9609) for BChE, indicating moderate enzyme inhibition. These results suggest that Muscari armeniacum could serve as a valuable source of bioactive compounds with significant antioxidant and enzyme inhibitory activities. Its potential applications in the treatment of oxidative stress-related diseases and neurodegenerative disorders make it a promising candidate for further pharmacological studies. This research underscores the importance of exploring native plants for their medicinal and therapeutic potential, contributing to the development of alternative treatments for challenging health conditions.https://agrifoodscience.com/index.php/TURJAF/article/view/7355antioxidantsenzyme inhibitorymuscari armeniacumphenolicsacetylcholinesterase |
| spellingShingle | Fevzi Topal Firat Yılmaz Meryem Topal Ahmed Menevşeoğlu Exploring the Antioxidant and Neuroprotective Potential of Muscari armeniacum: Phenolic Profiling and Enzyme Inhibition Turkish Journal of Agriculture: Food Science and Technology antioxidants enzyme inhibitory muscari armeniacum phenolics acetylcholinesterase |
| title | Exploring the Antioxidant and Neuroprotective Potential of Muscari armeniacum: Phenolic Profiling and Enzyme Inhibition |
| title_full | Exploring the Antioxidant and Neuroprotective Potential of Muscari armeniacum: Phenolic Profiling and Enzyme Inhibition |
| title_fullStr | Exploring the Antioxidant and Neuroprotective Potential of Muscari armeniacum: Phenolic Profiling and Enzyme Inhibition |
| title_full_unstemmed | Exploring the Antioxidant and Neuroprotective Potential of Muscari armeniacum: Phenolic Profiling and Enzyme Inhibition |
| title_short | Exploring the Antioxidant and Neuroprotective Potential of Muscari armeniacum: Phenolic Profiling and Enzyme Inhibition |
| title_sort | exploring the antioxidant and neuroprotective potential of muscari armeniacum phenolic profiling and enzyme inhibition |
| topic | antioxidants enzyme inhibitory muscari armeniacum phenolics acetylcholinesterase |
| url | https://agrifoodscience.com/index.php/TURJAF/article/view/7355 |
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