Short path molecular distillation of the essential oil from Pinus roxburghii oleoresin affords volatile fractions with powerful antioxidant and antimicrobial activities comparable with common synthetic agents and antimicrobials
The primary purposes of this work were to study the influence of the distillation temperature on the yield of the turpentine essential oil hydrodistilled from the Pinus roxburghii oleoresin and to obtain products with powerful bioactivities by short path molecular distillation of the oil. Our findin...
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Elsevier
2025-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025006620 |
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| author | Muhammad Adnan Ayub Hawraz Ibrahim M. Amin Rameen Waseem Kamaran Younis M. Amin Muhammad Asif Hanif Amjad Hussain Kovan Dilawar Issa Jorge Ramírez Chabaco Armijos Muhammad Zubair Giovanni Vidari |
| author_facet | Muhammad Adnan Ayub Hawraz Ibrahim M. Amin Rameen Waseem Kamaran Younis M. Amin Muhammad Asif Hanif Amjad Hussain Kovan Dilawar Issa Jorge Ramírez Chabaco Armijos Muhammad Zubair Giovanni Vidari |
| author_sort | Muhammad Adnan Ayub |
| collection | DOAJ |
| description | The primary purposes of this work were to study the influence of the distillation temperature on the yield of the turpentine essential oil hydrodistilled from the Pinus roxburghii oleoresin and to obtain products with powerful bioactivities by short path molecular distillation of the oil. Our findings showed that the hydrodistillation temperature affected the yield as well as the antioxidant and antimicrobial properties of the essential oil: the highest yield (20.27 %) and bioactivities were obtained for the oil distilled at 180 °C. The oil was subsequently separated by short path molecular distillation into fractions and sub-fractions whose antioxidant activity was determined by the DPPH scavenging assay, the inhibition of linoleic acid peroxidation test, the FRAP assay, and the H2O2 scavenging assays. In addition, the antimicrobial activity of the fractions and sub-fractions against the bacteria Pasteurella multocida, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis, and the fungal strains Fusarium solani, Alternaria alternata, Aspergillus flavus, and Aspergillus niger, was measured by the agar well diffusion method, the microdilution broth assay and the resazurin microtiter plate test. The antioxidant and antimicrobial activities varied between fractions and subfractions; however, their overall potency makes the P. roxburghii oleoresin a potential source of valuable natural antimicrobial products employable against various foodborne microbes and molds, as well as a source of preservative agents against food oxidation. Longifolene, 3-carene, α-pinene and β-pinene were identified by GC-MS analysis as the main constituents of the P. roxburghii turpentine essential oil and the most active fractions and sub-fractions. These terpenes were likely responsible for the bioactivity of the volatile mixtures. |
| format | Article |
| id | doaj-art-b07552466d0f4f9dad9b845f16873dce |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-b07552466d0f4f9dad9b845f16873dce2025-02-06T05:12:35ZengElsevierHeliyon2405-84402025-02-01113e42282Short path molecular distillation of the essential oil from Pinus roxburghii oleoresin affords volatile fractions with powerful antioxidant and antimicrobial activities comparable with common synthetic agents and antimicrobialsMuhammad Adnan Ayub0Hawraz Ibrahim M. Amin1Rameen Waseem2Kamaran Younis M. Amin3Muhammad Asif Hanif4Amjad Hussain5Kovan Dilawar Issa6Jorge Ramírez7Chabaco Armijos8Muhammad Zubair9Giovanni Vidari10Department of Chemistry, University of Sahiwal, 57000, Sahiwal, Pakistan; Corresponding author.Department of Chemistry, College of Science, Salahaddin University-Erbil, Erbil, 44001, Iraq; Department of Medical Biochemical Analysis, Cihan University-Erbil, Erbil, 44001, Iraq; Corresponding author. Department of Chemistry, College of Science, Salahaddin University-Erbil, Erbil, 44001, Iraq.Department of Chemistry, University of Sahiwal, 57000, Sahiwal, PakistanDepartment of Chemistry, College of Education, Salahaddin University-Erbil, Erbil, 44001, IraqDepartment of Chemistry, University of Agriculture Faisalabad, Faisalabad, 38000, PakistanInstitute of Chemistry, University of Okara, Okara, 56300, Punjab, PakistanDepartment of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil, 44001, IraqDepartamento de Química, Universidad Técnica Particular de Loja, Loja, 110107, EcuadorDepartamento de Química, Universidad Técnica Particular de Loja, Loja, 110107, EcuadorDepartment of Chemistry, Faculty of Science, University of Gujrat, Gujrat, 50700, PakistanDepartment of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil, 44001, Iraq; Corresponding author.The primary purposes of this work were to study the influence of the distillation temperature on the yield of the turpentine essential oil hydrodistilled from the Pinus roxburghii oleoresin and to obtain products with powerful bioactivities by short path molecular distillation of the oil. Our findings showed that the hydrodistillation temperature affected the yield as well as the antioxidant and antimicrobial properties of the essential oil: the highest yield (20.27 %) and bioactivities were obtained for the oil distilled at 180 °C. The oil was subsequently separated by short path molecular distillation into fractions and sub-fractions whose antioxidant activity was determined by the DPPH scavenging assay, the inhibition of linoleic acid peroxidation test, the FRAP assay, and the H2O2 scavenging assays. In addition, the antimicrobial activity of the fractions and sub-fractions against the bacteria Pasteurella multocida, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis, and the fungal strains Fusarium solani, Alternaria alternata, Aspergillus flavus, and Aspergillus niger, was measured by the agar well diffusion method, the microdilution broth assay and the resazurin microtiter plate test. The antioxidant and antimicrobial activities varied between fractions and subfractions; however, their overall potency makes the P. roxburghii oleoresin a potential source of valuable natural antimicrobial products employable against various foodborne microbes and molds, as well as a source of preservative agents against food oxidation. Longifolene, 3-carene, α-pinene and β-pinene were identified by GC-MS analysis as the main constituents of the P. roxburghii turpentine essential oil and the most active fractions and sub-fractions. These terpenes were likely responsible for the bioactivity of the volatile mixtures.http://www.sciencedirect.com/science/article/pii/S2405844025006620Chir pine oleoresinEssential oilOil fractionation by molecular distillationGC-MS analysisAntioxidant and antimicrobial activities |
| spellingShingle | Muhammad Adnan Ayub Hawraz Ibrahim M. Amin Rameen Waseem Kamaran Younis M. Amin Muhammad Asif Hanif Amjad Hussain Kovan Dilawar Issa Jorge Ramírez Chabaco Armijos Muhammad Zubair Giovanni Vidari Short path molecular distillation of the essential oil from Pinus roxburghii oleoresin affords volatile fractions with powerful antioxidant and antimicrobial activities comparable with common synthetic agents and antimicrobials Heliyon Chir pine oleoresin Essential oil Oil fractionation by molecular distillation GC-MS analysis Antioxidant and antimicrobial activities |
| title | Short path molecular distillation of the essential oil from Pinus roxburghii oleoresin affords volatile fractions with powerful antioxidant and antimicrobial activities comparable with common synthetic agents and antimicrobials |
| title_full | Short path molecular distillation of the essential oil from Pinus roxburghii oleoresin affords volatile fractions with powerful antioxidant and antimicrobial activities comparable with common synthetic agents and antimicrobials |
| title_fullStr | Short path molecular distillation of the essential oil from Pinus roxburghii oleoresin affords volatile fractions with powerful antioxidant and antimicrobial activities comparable with common synthetic agents and antimicrobials |
| title_full_unstemmed | Short path molecular distillation of the essential oil from Pinus roxburghii oleoresin affords volatile fractions with powerful antioxidant and antimicrobial activities comparable with common synthetic agents and antimicrobials |
| title_short | Short path molecular distillation of the essential oil from Pinus roxburghii oleoresin affords volatile fractions with powerful antioxidant and antimicrobial activities comparable with common synthetic agents and antimicrobials |
| title_sort | short path molecular distillation of the essential oil from pinus roxburghii oleoresin affords volatile fractions with powerful antioxidant and antimicrobial activities comparable with common synthetic agents and antimicrobials |
| topic | Chir pine oleoresin Essential oil Oil fractionation by molecular distillation GC-MS analysis Antioxidant and antimicrobial activities |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025006620 |
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