Evaluating the efficiency of 16S-ITS-23S operon sequencing for species level resolution in microbial communities

Evaluating the efficiency of 16S-ITS-23S operon sequencing for species level resolution in microbial communities

Abstract Rapid advancements in long-read sequencing have facilitated species-level microbial profiling through full-length 16S rRNA sequencing (~ 1500 bp), and more notably, by the newer 16S-ITS-23S ribosomal RNA operon (RRN) sequencing (~ 4500 bp). RRN sequencing is emerging as a superior method fo...

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Bibliographic Details
Main Authors: Meghana Srinivas, Calum J. Walsh, Fiona Crispie, Orla O’Sullivan, Paul D. Cotter, Douwe van Sinderen, John G. Kenny
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Subjects:
RRN operon
16S-ITS-23S
long-read sequencing
PacBio
Oxford Nanopore Technology (ONT)
16S rRNA sequencing
Online Access:https://doi.org/10.1038/s41598-024-83410-7
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