Dynamics of the genetic diversity of oat varieties in the Tyumen region at avenin-coding loci
Molecular and biochemical markers are used to analyze the intraspecific genetic diversity of crops. Prolamincoding loci are highly effective for assessing this indicator. On the basis of the Laboratory of Varietal Seed Identification of the State Agrarian University of the Northern Trans-Urals, 18...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2020-04-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2541 |
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| author | A. V. Lyubimova G. V. Tobolova D. I. Eremin I. G. Loskutov |
| author_facet | A. V. Lyubimova G. V. Tobolova D. I. Eremin I. G. Loskutov |
| author_sort | A. V. Lyubimova |
| collection | DOAJ |
| description | Molecular and biochemical markers are used to analyze the intraspecific genetic diversity of crops. Prolamincoding loci are highly effective for assessing this indicator. On the basis of the Laboratory of Varietal Seed Identification of the State Agrarian University of the Northern Trans-Urals, 18 varieties of common oat included in the State Register of Selection Achievements in the Tyumen Region from the 1930s to 2019 were studied by electrophoresis in 2018–2019. The aim of the work was to study the dynamics of the genetic diversity of oat va rieties at avenin-coding loci. For the analysis, 100 grains of each variety were used. Electrophoresis was carried out in vertical plates of 13.2 % polyacrylamide gel at a constant vol tage of 500 V for 4.0–4.5 h. It was found that 44.4 % of the varieties are heterogeneous, each consisting of two biotypes. For three loci, 20 alleles were identified, 10 of which were detected for the first time. The allele frequency of avenin-coding loci varied with time. In the process of variety exchange, alleles that are characteristic of varieties of non-Russian origin were replaced by alleles present in domestic varieties and then in the varieties developed by local breeding institutions. The following alleles had the highest frequency in Tyumen varieties: Avn A4(50.0 %), A2(25.0 %), Avn B4(50.0 %), Bnew6(37.5 %), Avn C1(37.5 %), C2 and C5(25.0 %). These alleles are of great value as markers of agronomically and adaptively important characters for the region in question. The amount of genetic diversity of oats varied with time from 0.33 in 1929–1950 to up to 0.75 in 2019. The high value of genetic diversity in modern breeding varieties of the Scientific Research Institute of Agriculture of the Northern Trans-Urals and an increase in this indicator over the past 20 years are associated with the use of genetically heterogeneous source material in the breeding process. This allowed obtaining varieties with high adaptive potentials in the natural climatic conditions of the region. |
| format | Article |
| id | doaj-art-b048e36371824890874eef71823bd57e |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-b048e36371824890874eef71823bd57e2025-02-01T09:58:08ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592020-04-0124212313010.18699/VJ20.6071035Dynamics of the genetic diversity of oat varieties in the Tyumen region at avenin-coding lociA. V. Lyubimova0G. V. Tobolova1D. I. Eremin2I. G. Loskutov3Scientific Research Institute of Agriculture of the Northern Trans-Ural Region – Branch of the Tyumen Scientific Center of Siberian Branch of the Russian Academy of Sciences; Northern Trans-Ural State Agricultural UniversityNorthern Trans-Ural State Agricultural UniversityNorthern Trans-Ural State Agricultural UniversityFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Molecular and biochemical markers are used to analyze the intraspecific genetic diversity of crops. Prolamincoding loci are highly effective for assessing this indicator. On the basis of the Laboratory of Varietal Seed Identification of the State Agrarian University of the Northern Trans-Urals, 18 varieties of common oat included in the State Register of Selection Achievements in the Tyumen Region from the 1930s to 2019 were studied by electrophoresis in 2018–2019. The aim of the work was to study the dynamics of the genetic diversity of oat va rieties at avenin-coding loci. For the analysis, 100 grains of each variety were used. Electrophoresis was carried out in vertical plates of 13.2 % polyacrylamide gel at a constant vol tage of 500 V for 4.0–4.5 h. It was found that 44.4 % of the varieties are heterogeneous, each consisting of two biotypes. For three loci, 20 alleles were identified, 10 of which were detected for the first time. The allele frequency of avenin-coding loci varied with time. In the process of variety exchange, alleles that are characteristic of varieties of non-Russian origin were replaced by alleles present in domestic varieties and then in the varieties developed by local breeding institutions. The following alleles had the highest frequency in Tyumen varieties: Avn A4(50.0 %), A2(25.0 %), Avn B4(50.0 %), Bnew6(37.5 %), Avn C1(37.5 %), C2 and C5(25.0 %). These alleles are of great value as markers of agronomically and adaptively important characters for the region in question. The amount of genetic diversity of oats varied with time from 0.33 in 1929–1950 to up to 0.75 in 2019. The high value of genetic diversity in modern breeding varieties of the Scientific Research Institute of Agriculture of the Northern Trans-Urals and an increase in this indicator over the past 20 years are associated with the use of genetically heterogeneous source material in the breeding process. This allowed obtaining varieties with high adaptive potentials in the natural climatic conditions of the region.https://vavilov.elpub.ru/jour/article/view/2541varietyelectrophoresisstorage proteinsaveninavenin-coding lociallelesgenetic diversity |
| spellingShingle | A. V. Lyubimova G. V. Tobolova D. I. Eremin I. G. Loskutov Dynamics of the genetic diversity of oat varieties in the Tyumen region at avenin-coding loci Вавиловский журнал генетики и селекции variety electrophoresis storage proteins avenin avenin-coding loci alleles genetic diversity |
| title | Dynamics of the genetic diversity of oat varieties in the Tyumen region at avenin-coding loci |
| title_full | Dynamics of the genetic diversity of oat varieties in the Tyumen region at avenin-coding loci |
| title_fullStr | Dynamics of the genetic diversity of oat varieties in the Tyumen region at avenin-coding loci |
| title_full_unstemmed | Dynamics of the genetic diversity of oat varieties in the Tyumen region at avenin-coding loci |
| title_short | Dynamics of the genetic diversity of oat varieties in the Tyumen region at avenin-coding loci |
| title_sort | dynamics of the genetic diversity of oat varieties in the tyumen region at avenin coding loci |
| topic | variety electrophoresis storage proteins avenin avenin-coding loci alleles genetic diversity |
| url | https://vavilov.elpub.ru/jour/article/view/2541 |
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