Pan-Cancer Analysis of Disulfidptosis-Related Genes Affecting Prognosis and Tumor Microenvironment
ObjectiveTo assess the potential role of disulfidptosis-related genes (DRGs) in pan-cancer on prognosis and immunity on the basis of bioinformatics approaches. MethodsPan-cancer RNA-seq data, mutation profiles, clinical information, TMB, MSI, stemness scores, and tumor and immune microenvironment da...
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Magazine House of Cancer Research on Prevention and Treatment
2025-01-01
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| Series: | Zhongliu Fangzhi Yanjiu |
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| Online Access: | http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.0302 |
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| author | Jingyang SUN Rongxuan JIANG Liren HOU Huanhuan DONG Yihan LIN Niuniu DONG Guangjian ZHANG Yanpeng ZHANG |
| author_facet | Jingyang SUN Rongxuan JIANG Liren HOU Huanhuan DONG Yihan LIN Niuniu DONG Guangjian ZHANG Yanpeng ZHANG |
| author_sort | Jingyang SUN |
| collection | DOAJ |
| description | ObjectiveTo assess the potential role of disulfidptosis-related genes (DRGs) in pan-cancer on prognosis and immunity on the basis of bioinformatics approaches. MethodsPan-cancer RNA-seq data, mutation profiles, clinical information, TMB, MSI, stemness scores, and tumor and immune microenvironment data contained in TCGA and various open-source online databases, and multi-group R-language algorithms were used for comprehensive analysis. The expression levels of DRGs at the cellular level were experimentally validated using qPCR. ResultsLRPPRC, NCKAP1, NDUFS1, and NUBPL had a better prognosis in renal clear cell carcinoma (P<0.001), whereas SLC7A11, NCKAP1, and SLC3A2 had a worse prognosis in hepatocellular carcinoma (P<0.001). TME analysis showed that LRPPRC was negatively correlated with immune cells, stromal cells, and estimated scores in all tumor types. TMB analysis revealed the potential research value of DRGs for PD-1/PD-L1 therapy in pan-cancer. Drug sensitivity analysis showed that SLC7A11 (r=0.454), SLC3A2 (r=0.366), and NCKAP1 (r=0.455) were significantly associated with Kahalide F (P<0.01). Experimental validation demonstrated the overall higher expression levels of GYS1 and NCKAP1 than normal cells in lung adenocarcinoma, colon adenocarcinoma, esophageal squamous carcinoma, and hepatocellular carcinoma (P<0.05). ConclusionPan-cancer analysis of DRGs indicates that DRGs may serve as important biomarkers for the diagnosis and prognosis of renal clear-cell carcinoma, lung adenocarcinoma, and hepatocellular carcinoma. |
| format | Article |
| id | doaj-art-b03fba6fb3bf4e2d9e1df940575d3226 |
| institution | Kabale University |
| issn | 1000-8578 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj-art-b03fba6fb3bf4e2d9e1df940575d32262025-01-23T07:44:48ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782025-01-01521526110.3971/j.issn.1000-8578.2025.24.030220240302Pan-Cancer Analysis of Disulfidptosis-Related Genes Affecting Prognosis and Tumor MicroenvironmentJingyang SUN0Rongxuan JIANG1Liren HOU2Huanhuan DONG3Yihan LIN4Niuniu DONG5Guangjian ZHANG6Yanpeng ZHANG7Department of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710000, ChinaDepartment of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710000, ChinaDepartment of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710000, ChinaDepartment of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710000, ChinaDepartment of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710000, ChinaDepartment of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710000, ChinaDepartment of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710000, ChinaDepartment of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710000, ChinaObjectiveTo assess the potential role of disulfidptosis-related genes (DRGs) in pan-cancer on prognosis and immunity on the basis of bioinformatics approaches. MethodsPan-cancer RNA-seq data, mutation profiles, clinical information, TMB, MSI, stemness scores, and tumor and immune microenvironment data contained in TCGA and various open-source online databases, and multi-group R-language algorithms were used for comprehensive analysis. The expression levels of DRGs at the cellular level were experimentally validated using qPCR. ResultsLRPPRC, NCKAP1, NDUFS1, and NUBPL had a better prognosis in renal clear cell carcinoma (P<0.001), whereas SLC7A11, NCKAP1, and SLC3A2 had a worse prognosis in hepatocellular carcinoma (P<0.001). TME analysis showed that LRPPRC was negatively correlated with immune cells, stromal cells, and estimated scores in all tumor types. TMB analysis revealed the potential research value of DRGs for PD-1/PD-L1 therapy in pan-cancer. Drug sensitivity analysis showed that SLC7A11 (r=0.454), SLC3A2 (r=0.366), and NCKAP1 (r=0.455) were significantly associated with Kahalide F (P<0.01). Experimental validation demonstrated the overall higher expression levels of GYS1 and NCKAP1 than normal cells in lung adenocarcinoma, colon adenocarcinoma, esophageal squamous carcinoma, and hepatocellular carcinoma (P<0.05). ConclusionPan-cancer analysis of DRGs indicates that DRGs may serve as important biomarkers for the diagnosis and prognosis of renal clear-cell carcinoma, lung adenocarcinoma, and hepatocellular carcinoma.http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.0302disulfidptosis-related genepan-cancer analysistumor microenvironmentdrug sensitivity |
| spellingShingle | Jingyang SUN Rongxuan JIANG Liren HOU Huanhuan DONG Yihan LIN Niuniu DONG Guangjian ZHANG Yanpeng ZHANG Pan-Cancer Analysis of Disulfidptosis-Related Genes Affecting Prognosis and Tumor Microenvironment Zhongliu Fangzhi Yanjiu disulfidptosis-related gene pan-cancer analysis tumor microenvironment drug sensitivity |
| title | Pan-Cancer Analysis of Disulfidptosis-Related Genes Affecting Prognosis and Tumor Microenvironment |
| title_full | Pan-Cancer Analysis of Disulfidptosis-Related Genes Affecting Prognosis and Tumor Microenvironment |
| title_fullStr | Pan-Cancer Analysis of Disulfidptosis-Related Genes Affecting Prognosis and Tumor Microenvironment |
| title_full_unstemmed | Pan-Cancer Analysis of Disulfidptosis-Related Genes Affecting Prognosis and Tumor Microenvironment |
| title_short | Pan-Cancer Analysis of Disulfidptosis-Related Genes Affecting Prognosis and Tumor Microenvironment |
| title_sort | pan cancer analysis of disulfidptosis related genes affecting prognosis and tumor microenvironment |
| topic | disulfidptosis-related gene pan-cancer analysis tumor microenvironment drug sensitivity |
| url | http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.0302 |
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