Blastomyces dermatitidis Yeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis
The systemic fungal infection, blastomycosis, which infects both humans and animals has presented a diagnostic challenge for clinicians for many years. The aim of this study was to evaluate the diagnostic sensitivity of Blastomyces dermatitidis yeast lysate antigens with respect to antibody detectio...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2013/940126 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556085556281344 |
|---|---|
| author | Alex R. Boyd Jamie L. VanDyke Gene M. Scalarone |
| author_facet | Alex R. Boyd Jamie L. VanDyke Gene M. Scalarone |
| author_sort | Alex R. Boyd |
| collection | DOAJ |
| description | The systemic fungal infection, blastomycosis, which infects both humans and animals has presented a diagnostic challenge for clinicians for many years. The aim of this study was to evaluate the diagnostic sensitivity of Blastomyces dermatitidis yeast lysate antigens with respect to antibody detection in dogs with blastomycosis. Lysate antigens were prepared from B. dermatitidis isolates T-58 and T-66 (dogs, Tennessee) and WI-R and WI-J (dogs, Wisconsin). Based on results obtained from a preliminary comparative study, five combinations of these isolates and one individual isolate were tested against 92 serum specimens from dogs with culture-proven or histologically-confirmed blastomycosis, using the indirect enzyme-linked immunosorbent assay (ELISA). Mean absorbance values obtained from the sera ranged from 0.905 with the individual T-58 antigen to 1.760 using an antigen combination (T-58 + T-66 + WI-R). All of the 6 antigenic preparations were able to detect antibody in the serum specimens, but the antigen combinations detected antibody to a higher degree than the individual antigen. This study provides evidence that combinations of the yeast lysate reagents seem to be more efficacious for antibody detection in dog sera, but our laboratory is continuing to evaluate antigen lysate combinations for detection of antibodies in blastomycosis. |
| format | Article |
| id | doaj-art-b0329ffe064f4f8b81b6b8168ec89d96 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-b0329ffe064f4f8b81b6b8168ec89d962025-02-03T05:46:26ZengWileyVeterinary Medicine International2090-81132042-00482013-01-01201310.1155/2013/940126940126Blastomyces dermatitidis Yeast Lysate Antigen Combinations: Antibody Detection in Dogs with BlastomycosisAlex R. Boyd0Jamie L. VanDyke1Gene M. Scalarone2Department of Biological Sciences, Idaho State University, P.O. Box 8007 921 S. 8th Avenue, Pocatello, ID 83209, USADepartment of Biological Sciences, Idaho State University, P.O. Box 8007 921 S. 8th Avenue, Pocatello, ID 83209, USADepartment of Biological Sciences, Idaho State University, P.O. Box 8007 921 S. 8th Avenue, Pocatello, ID 83209, USAThe systemic fungal infection, blastomycosis, which infects both humans and animals has presented a diagnostic challenge for clinicians for many years. The aim of this study was to evaluate the diagnostic sensitivity of Blastomyces dermatitidis yeast lysate antigens with respect to antibody detection in dogs with blastomycosis. Lysate antigens were prepared from B. dermatitidis isolates T-58 and T-66 (dogs, Tennessee) and WI-R and WI-J (dogs, Wisconsin). Based on results obtained from a preliminary comparative study, five combinations of these isolates and one individual isolate were tested against 92 serum specimens from dogs with culture-proven or histologically-confirmed blastomycosis, using the indirect enzyme-linked immunosorbent assay (ELISA). Mean absorbance values obtained from the sera ranged from 0.905 with the individual T-58 antigen to 1.760 using an antigen combination (T-58 + T-66 + WI-R). All of the 6 antigenic preparations were able to detect antibody in the serum specimens, but the antigen combinations detected antibody to a higher degree than the individual antigen. This study provides evidence that combinations of the yeast lysate reagents seem to be more efficacious for antibody detection in dog sera, but our laboratory is continuing to evaluate antigen lysate combinations for detection of antibodies in blastomycosis.http://dx.doi.org/10.1155/2013/940126 |
| spellingShingle | Alex R. Boyd Jamie L. VanDyke Gene M. Scalarone Blastomyces dermatitidis Yeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis Veterinary Medicine International |
| title | Blastomyces dermatitidis Yeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis |
| title_full | Blastomyces dermatitidis Yeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis |
| title_fullStr | Blastomyces dermatitidis Yeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis |
| title_full_unstemmed | Blastomyces dermatitidis Yeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis |
| title_short | Blastomyces dermatitidis Yeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis |
| title_sort | blastomyces dermatitidis yeast lysate antigen combinations antibody detection in dogs with blastomycosis |
| url | http://dx.doi.org/10.1155/2013/940126 |
| work_keys_str_mv | AT alexrboyd blastomycesdermatitidisyeastlysateantigencombinationsantibodydetectionindogswithblastomycosis AT jamielvandyke blastomycesdermatitidisyeastlysateantigencombinationsantibodydetectionindogswithblastomycosis AT genemscalarone blastomycesdermatitidisyeastlysateantigencombinationsantibodydetectionindogswithblastomycosis |