Comparative transcriptomics and metabolomics provide insight into degeneration‐related physiological mechanisms of Morchella importuna after long‐term preservation
Abstract Ascomycetes fungi are often prone to degeneration. Agricultural production of the prized ascomycete mushroom Morchella importuna (black morel) typically suffers from reduced yield and malformed ascocarps owing to culture degeneration. This study compared M. importuna cultures subjected to f...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.70045 |
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| author | Ying Chen Xuelian Cao Liyuan Xie Jie Tang Lixu Liu Di Wang Xiang Wu Tianhai Liu Yang Yu Yong Wang Francis Martin Weihong Peng Hao Tan |
| author_facet | Ying Chen Xuelian Cao Liyuan Xie Jie Tang Lixu Liu Di Wang Xiang Wu Tianhai Liu Yang Yu Yong Wang Francis Martin Weihong Peng Hao Tan |
| author_sort | Ying Chen |
| collection | DOAJ |
| description | Abstract Ascomycetes fungi are often prone to degeneration. Agricultural production of the prized ascomycete mushroom Morchella importuna (black morel) typically suffers from reduced yield and malformed ascocarps owing to culture degeneration. This study compared M. importuna cultures subjected to five different long‐term preservation treatments, using transcriptomics and metabolomics. Avoiding repeated subculturing in combination with nutrient‐limited conditions was found to be the most beneficial method for maintaining the fruiting capability of morels. The expression of the gene sets involved in cysteine and methionine metabolism and nucleocytoplasmic transport was upregulated under nutrient‐limited and nutrient‐rich conditions, respectively. This increased expression was accompanied by differential accumulation of metabolites involved in nucleobase metabolism. Repeated subculturing triggered dissimilar changes in the functional modules under nutrient‐rich and nutrient‐limited conditions. A diverse set of cellular biochemical processes related to carbon metabolism were altered by repeated subculturing under nutrient‐rich conditions, whereas glycerophospholipid and purine metabolism were key functions affected by repeated subculturing under nutrient‐limited conditions. Altogether, metabolic alterations related to sulfur‐containing amino‐acid biosynthesis, DNA repair, and cellular structural maintenance contributed to improved preservation outcomes in terms of morel fruiting capability. Our findings contribute to a more detailed understanding of the molecular mechanisms related to subculturing and fruiting of ascomycete macrofungi after long‐term preservation. |
| format | Article |
| id | doaj-art-b02c740b6d654f9593eeb43b05126a62 |
| institution | Kabale University |
| issn | 1751-7915 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj-art-b02c740b6d654f9593eeb43b05126a622025-01-31T06:26:35ZengWileyMicrobial Biotechnology1751-79152025-01-01181n/an/a10.1111/1751-7915.70045Comparative transcriptomics and metabolomics provide insight into degeneration‐related physiological mechanisms of Morchella importuna after long‐term preservationYing Chen0Xuelian Cao1Liyuan Xie2Jie Tang3Lixu Liu4Di Wang5Xiang Wu6Tianhai Liu7Yang Yu8Yong Wang9Francis Martin10Weihong Peng11Hao Tan12Sichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaThe National Key Laboratory of Ecological Security and Sustainable Development in Arid Region, Northwest Institute of Eco‐Environment and Resources Chinese Academy of Sciences Lanzhou ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaSichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Chengdu ChinaAbstract Ascomycetes fungi are often prone to degeneration. Agricultural production of the prized ascomycete mushroom Morchella importuna (black morel) typically suffers from reduced yield and malformed ascocarps owing to culture degeneration. This study compared M. importuna cultures subjected to five different long‐term preservation treatments, using transcriptomics and metabolomics. Avoiding repeated subculturing in combination with nutrient‐limited conditions was found to be the most beneficial method for maintaining the fruiting capability of morels. The expression of the gene sets involved in cysteine and methionine metabolism and nucleocytoplasmic transport was upregulated under nutrient‐limited and nutrient‐rich conditions, respectively. This increased expression was accompanied by differential accumulation of metabolites involved in nucleobase metabolism. Repeated subculturing triggered dissimilar changes in the functional modules under nutrient‐rich and nutrient‐limited conditions. A diverse set of cellular biochemical processes related to carbon metabolism were altered by repeated subculturing under nutrient‐rich conditions, whereas glycerophospholipid and purine metabolism were key functions affected by repeated subculturing under nutrient‐limited conditions. Altogether, metabolic alterations related to sulfur‐containing amino‐acid biosynthesis, DNA repair, and cellular structural maintenance contributed to improved preservation outcomes in terms of morel fruiting capability. Our findings contribute to a more detailed understanding of the molecular mechanisms related to subculturing and fruiting of ascomycete macrofungi after long‐term preservation.https://doi.org/10.1111/1751-7915.70045 |
| spellingShingle | Ying Chen Xuelian Cao Liyuan Xie Jie Tang Lixu Liu Di Wang Xiang Wu Tianhai Liu Yang Yu Yong Wang Francis Martin Weihong Peng Hao Tan Comparative transcriptomics and metabolomics provide insight into degeneration‐related physiological mechanisms of Morchella importuna after long‐term preservation Microbial Biotechnology |
| title | Comparative transcriptomics and metabolomics provide insight into degeneration‐related physiological mechanisms of Morchella importuna after long‐term preservation |
| title_full | Comparative transcriptomics and metabolomics provide insight into degeneration‐related physiological mechanisms of Morchella importuna after long‐term preservation |
| title_fullStr | Comparative transcriptomics and metabolomics provide insight into degeneration‐related physiological mechanisms of Morchella importuna after long‐term preservation |
| title_full_unstemmed | Comparative transcriptomics and metabolomics provide insight into degeneration‐related physiological mechanisms of Morchella importuna after long‐term preservation |
| title_short | Comparative transcriptomics and metabolomics provide insight into degeneration‐related physiological mechanisms of Morchella importuna after long‐term preservation |
| title_sort | comparative transcriptomics and metabolomics provide insight into degeneration related physiological mechanisms of morchella importuna after long term preservation |
| url | https://doi.org/10.1111/1751-7915.70045 |
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