Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis
IntroductionExtracellular vesicles (EVs) shed from tumor cells into peripheral circulation or other body fluids are promising biomarkers for cancer diagnosis with enormously long circulation. Consequently, precise methods for differentiating normal and tumor-associated EVs (TAEs) are required.Method...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2025.1531108/full |
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| author | Xiao Du Xiao Du Xiao Du Hongxiu Li Hongxiu Li Hongxiu Li Shiyi Shen Shiyi Shen Shiyi Shen Chao Tian Chao Tian Chao Tian Xiaohuan Cao Xiaohuan Cao Xiaohuan Cao Xingang Xu Nan Xu Shuling Wang Shuling Wang Shuling Wang Qingchang Tian Qingchang Tian Qingchang Tian |
| author_facet | Xiao Du Xiao Du Xiao Du Hongxiu Li Hongxiu Li Hongxiu Li Shiyi Shen Shiyi Shen Shiyi Shen Chao Tian Chao Tian Chao Tian Xiaohuan Cao Xiaohuan Cao Xiaohuan Cao Xingang Xu Nan Xu Shuling Wang Shuling Wang Shuling Wang Qingchang Tian Qingchang Tian Qingchang Tian |
| author_sort | Xiao Du |
| collection | DOAJ |
| description | IntroductionExtracellular vesicles (EVs) shed from tumor cells into peripheral circulation or other body fluids are promising biomarkers for cancer diagnosis with enormously long circulation. Consequently, precise methods for differentiating normal and tumor-associated EVs (TAEs) are required.MethodsThis study used quantifiable antibody-DNA conjugate-assisted quantitative methods combined with proximity ligation technology to detect TAEs. The antibody-DNA conjugate contained one antibody associated with three oligonucleotides for signal amplification. The antibody in the conjugate can recognize the surface tumor antigens of TAEs. Simultaneously, DNA in the conjugate is attached to the surfaces of TAEs and holds the signal amplification post, converting protein identities to DNA amplification for protein detection, even at the molecular level.ResultsThese findings revealed that TAEs can be quantitatively detected using DNA-mediated quantitative polymerase chain reaction (qPCR). Antibody-DNA conjugates were used to recognize the epithelial cell adhesion molecule (EpCAM) antigen on the TAE surface and quantify the antigen using qPCR for cancer analysis.DiscussionThis method proposed a new quantitative detection approach for TAEs, which aim to identify specific EV-associated markers for diagnostic or therapeutic, this method could inspire a new idea for tumor diagnosis and detection of other diseases. |
| format | Article |
| id | doaj-art-b0253a1cf44a49b5ac7b47278bdd45a0 |
| institution | Kabale University |
| issn | 2296-889X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj-art-b0253a1cf44a49b5ac7b47278bdd45a02025-01-22T05:19:29ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2025-01-011210.3389/fmolb.2025.15311081531108Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysisXiao Du0Xiao Du1Xiao Du2Hongxiu Li3Hongxiu Li4Hongxiu Li5Shiyi Shen6Shiyi Shen7Shiyi Shen8Chao Tian9Chao Tian10Chao Tian11Xiaohuan Cao12Xiaohuan Cao13Xiaohuan Cao14Xingang Xu15Nan Xu16Shuling Wang17Shuling Wang18Shuling Wang19Qingchang Tian20Qingchang Tian21Qingchang Tian22School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaEngineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaEngineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaEngineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaEngineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaEngineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaLaboratory of Chinese Medicine Preparation, Shandong Research Academy of Traditional Chinese Medicine, Jinan, ChinaLaboratory of Chinese Medicine Preparation, Shandong Research Academy of Traditional Chinese Medicine, Jinan, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaEngineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaKey Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaEngineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaIntroductionExtracellular vesicles (EVs) shed from tumor cells into peripheral circulation or other body fluids are promising biomarkers for cancer diagnosis with enormously long circulation. Consequently, precise methods for differentiating normal and tumor-associated EVs (TAEs) are required.MethodsThis study used quantifiable antibody-DNA conjugate-assisted quantitative methods combined with proximity ligation technology to detect TAEs. The antibody-DNA conjugate contained one antibody associated with three oligonucleotides for signal amplification. The antibody in the conjugate can recognize the surface tumor antigens of TAEs. Simultaneously, DNA in the conjugate is attached to the surfaces of TAEs and holds the signal amplification post, converting protein identities to DNA amplification for protein detection, even at the molecular level.ResultsThese findings revealed that TAEs can be quantitatively detected using DNA-mediated quantitative polymerase chain reaction (qPCR). Antibody-DNA conjugates were used to recognize the epithelial cell adhesion molecule (EpCAM) antigen on the TAE surface and quantify the antigen using qPCR for cancer analysis.DiscussionThis method proposed a new quantitative detection approach for TAEs, which aim to identify specific EV-associated markers for diagnostic or therapeutic, this method could inspire a new idea for tumor diagnosis and detection of other diseases.https://www.frontiersin.org/articles/10.3389/fmolb.2025.1531108/fullextracellular vesiclesantibody-DNA conjugatesPoisson distributionproximity ligation technologytumor diagnosis |
| spellingShingle | Xiao Du Xiao Du Xiao Du Hongxiu Li Hongxiu Li Hongxiu Li Shiyi Shen Shiyi Shen Shiyi Shen Chao Tian Chao Tian Chao Tian Xiaohuan Cao Xiaohuan Cao Xiaohuan Cao Xingang Xu Nan Xu Shuling Wang Shuling Wang Shuling Wang Qingchang Tian Qingchang Tian Qingchang Tian Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis Frontiers in Molecular Biosciences extracellular vesicles antibody-DNA conjugates Poisson distribution proximity ligation technology tumor diagnosis |
| title | Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis |
| title_full | Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis |
| title_fullStr | Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis |
| title_full_unstemmed | Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis |
| title_short | Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis |
| title_sort | labeling tumor associated extracellular vesicles with antibody dna conjugates for quantitative analysis |
| topic | extracellular vesicles antibody-DNA conjugates Poisson distribution proximity ligation technology tumor diagnosis |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2025.1531108/full |
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