Protocol for cell image-based spatiotemporal proteomics in budding yeast
Summary: The eukaryotic cell division cycle is a highly conserved process, featuring fluctuations in protein localization and abundance required for key cell cycle transitions. Here, we present a protocol for the spatiotemporal analysis of the proteome during the budding yeast cell division cycle us...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007421 |
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| author | Athanasios Litsios Myra Paz David Masinas Helena Friesen Charles Boone Brenda J. Andrews |
| author_facet | Athanasios Litsios Myra Paz David Masinas Helena Friesen Charles Boone Brenda J. Andrews |
| author_sort | Athanasios Litsios |
| collection | DOAJ |
| description | Summary: The eukaryotic cell division cycle is a highly conserved process, featuring fluctuations in protein localization and abundance required for key cell cycle transitions. Here, we present a protocol for the spatiotemporal analysis of the proteome during the budding yeast cell division cycle using live-cell imaging. We describe steps for strain construction, cell cultivation, microscopy, and image analysis. Variations of this protocol can be applied for the spatiotemporal analysis of the proteome in different contexts, such as genetic and environmental perturbations.For complete details on the use and execution of this protocol, please refer to Litsios et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-b00e8387bb59411a844df9e743211bb3 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-b00e8387bb59411a844df9e743211bb32025-01-18T05:05:14ZengElsevierSTAR Protocols2666-16672025-03-0161103577Protocol for cell image-based spatiotemporal proteomics in budding yeastAthanasios Litsios0Myra Paz David Masinas1Helena Friesen2Charles Boone3Brenda J. Andrews4Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada; Corresponding authorDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada; Corresponding authorDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, CanadaDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada; RIKEN Center for Sustainable Resource Science, Wako Saitama 351-0198, Japan; Corresponding authorDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada; Corresponding authorSummary: The eukaryotic cell division cycle is a highly conserved process, featuring fluctuations in protein localization and abundance required for key cell cycle transitions. Here, we present a protocol for the spatiotemporal analysis of the proteome during the budding yeast cell division cycle using live-cell imaging. We describe steps for strain construction, cell cultivation, microscopy, and image analysis. Variations of this protocol can be applied for the spatiotemporal analysis of the proteome in different contexts, such as genetic and environmental perturbations.For complete details on the use and execution of this protocol, please refer to Litsios et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007421Cell BiologySingle CellMicroscopySystems biology |
| spellingShingle | Athanasios Litsios Myra Paz David Masinas Helena Friesen Charles Boone Brenda J. Andrews Protocol for cell image-based spatiotemporal proteomics in budding yeast STAR Protocols Cell Biology Single Cell Microscopy Systems biology |
| title | Protocol for cell image-based spatiotemporal proteomics in budding yeast |
| title_full | Protocol for cell image-based spatiotemporal proteomics in budding yeast |
| title_fullStr | Protocol for cell image-based spatiotemporal proteomics in budding yeast |
| title_full_unstemmed | Protocol for cell image-based spatiotemporal proteomics in budding yeast |
| title_short | Protocol for cell image-based spatiotemporal proteomics in budding yeast |
| title_sort | protocol for cell image based spatiotemporal proteomics in budding yeast |
| topic | Cell Biology Single Cell Microscopy Systems biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007421 |
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