Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables
The intestine integrity and function are crucial for nutrient absorption and to prevent the entry of harmful antigens. In sub-Saharan Africa, frequent infections and enteric dysfunction can cause intestinal inflammation that is associated with stunting. Therefore, to study the anti-inflammatory effe...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | NFS Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352364625000021 |
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| author | Nelly Fioroni Maria Del Carmen Ponce de León Rodríguez Nicolas Leconte Claire Mouquet-Rivier Caroline Guzman Frédéric Boudard Claudie Dhuique-Mayer Myriam Collin Anaïs Deglos Emmanuelle Reboul Ángela Bravo-Núñez Caroline Laurent-Babot |
| author_facet | Nelly Fioroni Maria Del Carmen Ponce de León Rodríguez Nicolas Leconte Claire Mouquet-Rivier Caroline Guzman Frédéric Boudard Claudie Dhuique-Mayer Myriam Collin Anaïs Deglos Emmanuelle Reboul Ángela Bravo-Núñez Caroline Laurent-Babot |
| author_sort | Nelly Fioroni |
| collection | DOAJ |
| description | The intestine integrity and function are crucial for nutrient absorption and to prevent the entry of harmful antigens. In sub-Saharan Africa, frequent infections and enteric dysfunction can cause intestinal inflammation that is associated with stunting. Therefore, to study the anti-inflammatory effects of cassava and roselle leaves, commonly consumed as sauces, we developed an in vitro triculture model of intestine inflammation using intestinal Caco-2 (colorectal adenocarcinoma) and HT29-MTX (goblet cell-like) together with the macrophage-like THP-1 cells. Stimulation of this model with lipopolysaccharide/interferon-γ resulted in mucus over-production, higher pro-inflammatory cytokine release and loss of intestinal barrier integrity due to increased permeability. Polar/non-polar extracts and digested sauces from cassava and roselle leaves reduced cytokine production in both intestinal and THP-1 cells to different extents and restored barrier integrity and permeability. The developed and validated triculture model of inflamed intestine thus demonstrated the anti-inflammatory properties of cassava and roselle leaves, despite moderate responses. |
| format | Article |
| id | doaj-art-b0052d5d4b8a409c9011dcc8b4a07503 |
| institution | Kabale University |
| issn | 2352-3646 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | NFS Journal |
| spelling | doaj-art-b0052d5d4b8a409c9011dcc8b4a075032025-01-20T04:17:32ZengElsevierNFS Journal2352-36462025-03-0138100213Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetablesNelly Fioroni0Maria Del Carmen Ponce de León Rodríguez1Nicolas Leconte2Claire Mouquet-Rivier3Caroline Guzman4Frédéric Boudard5Claudie Dhuique-Mayer6Myriam Collin7Anaïs Deglos8Emmanuelle Reboul9Ángela Bravo-Núñez10Caroline Laurent-Babot11UMR QualiSud, Université de Montpellier, Avignon Université, CIRAD, Institut Agro, IRD, Université de la Réunion, Montpellier, France; Corresponding authors.UMR QualiSud, Université de Montpellier, Avignon Université, CIRAD, Institut Agro, IRD, Université de la Réunion, Montpellier, FranceUMR HSM, Université de Montpellier, CNRS, IRD, Montpellier, FranceUMR QualiSud, Université de Montpellier, Avignon Université, CIRAD, Institut Agro, IRD, Université de la Réunion, Montpellier, FranceUMR QualiSud, Université de Montpellier, Avignon Université, CIRAD, Institut Agro, IRD, Université de la Réunion, Montpellier, FranceUMR QualiSud, Université de Montpellier, Avignon Université, CIRAD, Institut Agro, IRD, Université de la Réunion, Montpellier, FranceUMR QualiSud, Université de Montpellier, Avignon Université, CIRAD, Institut Agro, IRD, Université de la Réunion, Montpellier, France; CIRAD, UMR QualiSud, F-34398 Montpellier, FranceUMR DIADE, IRD/CIRAD F2F-Palmiers, Université de Montpellier, IRD, Montpellier, FranceUMR QualiSud, Université de Montpellier, Avignon Université, CIRAD, Institut Agro, IRD, Université de la Réunion, Montpellier, FranceAix-Marseille Université, INRAE, INSERM, C2VN, Marseille, FranceAix-Marseille Université, INRAE, INSERM, C2VN, Marseille, FranceUMR QualiSud, Université de Montpellier, Avignon Université, CIRAD, Institut Agro, IRD, Université de la Réunion, Montpellier, France; Corresponding authors.The intestine integrity and function are crucial for nutrient absorption and to prevent the entry of harmful antigens. In sub-Saharan Africa, frequent infections and enteric dysfunction can cause intestinal inflammation that is associated with stunting. Therefore, to study the anti-inflammatory effects of cassava and roselle leaves, commonly consumed as sauces, we developed an in vitro triculture model of intestine inflammation using intestinal Caco-2 (colorectal adenocarcinoma) and HT29-MTX (goblet cell-like) together with the macrophage-like THP-1 cells. Stimulation of this model with lipopolysaccharide/interferon-γ resulted in mucus over-production, higher pro-inflammatory cytokine release and loss of intestinal barrier integrity due to increased permeability. Polar/non-polar extracts and digested sauces from cassava and roselle leaves reduced cytokine production in both intestinal and THP-1 cells to different extents and restored barrier integrity and permeability. The developed and validated triculture model of inflamed intestine thus demonstrated the anti-inflammatory properties of cassava and roselle leaves, despite moderate responses.http://www.sciencedirect.com/science/article/pii/S2352364625000021Caco-2/HT29 bicultureTHP-1 macrophagesLeaf extractIn vitro digestionIntestinal permeabilityCytokines |
| spellingShingle | Nelly Fioroni Maria Del Carmen Ponce de León Rodríguez Nicolas Leconte Claire Mouquet-Rivier Caroline Guzman Frédéric Boudard Claudie Dhuique-Mayer Myriam Collin Anaïs Deglos Emmanuelle Reboul Ángela Bravo-Núñez Caroline Laurent-Babot Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables NFS Journal Caco-2/HT29 biculture THP-1 macrophages Leaf extract In vitro digestion Intestinal permeability Cytokines |
| title | Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables |
| title_full | Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables |
| title_fullStr | Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables |
| title_full_unstemmed | Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables |
| title_short | Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables |
| title_sort | development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle two african green leafy vegetables |
| topic | Caco-2/HT29 biculture THP-1 macrophages Leaf extract In vitro digestion Intestinal permeability Cytokines |
| url | http://www.sciencedirect.com/science/article/pii/S2352364625000021 |
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