High-Titer L-lactic Acid Production by Fed-Batch Simultaneous Saccharification and Fermentation of Steam-Exploded Corn Stover
Steam explosion (SE) is an effective lignocellulose pretreatment technology for second-generation L-lactic acid (L-LA) production. In this study, targeted to produce high-concentration L-LA from corn stover (CS), the fed-batch simultaneous saccharification and fermentation (SSF) of acidic, SE-pretre...
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MDPI AG
2025-01-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/11/1/25 |
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| author | Liheng Deng Changsheng Su Yilu Wu Qiang Xue Changwei Zhang Yong Wang Bin Wang Di Cai |
| author_facet | Liheng Deng Changsheng Su Yilu Wu Qiang Xue Changwei Zhang Yong Wang Bin Wang Di Cai |
| author_sort | Liheng Deng |
| collection | DOAJ |
| description | Steam explosion (SE) is an effective lignocellulose pretreatment technology for second-generation L-lactic acid (L-LA) production. In this study, targeted to produce high-concentration L-LA from corn stover (CS), the fed-batch simultaneous saccharification and fermentation (SSF) of acidic, SE-pretreated CS was developed and demonstrated in a 5 L scale bioreactor under non-strict conditions (without detoxification and sterilization). The results indicated that the fed-batch SSF, with a simple pH control, realized a higher tolerance of the strains to the toxic by-products of hydrolysate, in comparison to the conventional sequential hydrolysis and fermentation (SHF), allowing for 153.8 g L<sup>−1</sup> of L-LA production, along with a productivity of 1.83 g L<sup>−1</sup> h<sup>−1</sup> in a system with a total of 40% (<i>w</i>/<i>v</i>) solid loading. The mass balance indicated that up to 449 kg of L-LA can be obtained from 1 t of dried CS. It exhibited obvious superiorities and laid down a solid foundation for the industrialization of second-generation L-LA production. |
| format | Article |
| id | doaj-art-b0023f357a57447abf3be032ee46ecec |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-b0023f357a57447abf3be032ee46ecec2025-01-24T13:32:05ZengMDPI AGFermentation2311-56372025-01-011112510.3390/fermentation11010025High-Titer L-lactic Acid Production by Fed-Batch Simultaneous Saccharification and Fermentation of Steam-Exploded Corn StoverLiheng Deng0Changsheng Su1Yilu Wu2Qiang Xue3Changwei Zhang4Yong Wang5Bin Wang6Di Cai7National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaFermentation Technology Innovation Center of Hebei Province, College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaSteam explosion (SE) is an effective lignocellulose pretreatment technology for second-generation L-lactic acid (L-LA) production. In this study, targeted to produce high-concentration L-LA from corn stover (CS), the fed-batch simultaneous saccharification and fermentation (SSF) of acidic, SE-pretreated CS was developed and demonstrated in a 5 L scale bioreactor under non-strict conditions (without detoxification and sterilization). The results indicated that the fed-batch SSF, with a simple pH control, realized a higher tolerance of the strains to the toxic by-products of hydrolysate, in comparison to the conventional sequential hydrolysis and fermentation (SHF), allowing for 153.8 g L<sup>−1</sup> of L-LA production, along with a productivity of 1.83 g L<sup>−1</sup> h<sup>−1</sup> in a system with a total of 40% (<i>w</i>/<i>v</i>) solid loading. The mass balance indicated that up to 449 kg of L-LA can be obtained from 1 t of dried CS. It exhibited obvious superiorities and laid down a solid foundation for the industrialization of second-generation L-LA production.https://www.mdpi.com/2311-5637/11/1/25steam explosionL-lactic acidsimultaneous saccharification and fermentationfed-batch hydrolysis<i>Bacillus coagulans</i> |
| spellingShingle | Liheng Deng Changsheng Su Yilu Wu Qiang Xue Changwei Zhang Yong Wang Bin Wang Di Cai High-Titer L-lactic Acid Production by Fed-Batch Simultaneous Saccharification and Fermentation of Steam-Exploded Corn Stover Fermentation steam explosion L-lactic acid simultaneous saccharification and fermentation fed-batch hydrolysis <i>Bacillus coagulans</i> |
| title | High-Titer L-lactic Acid Production by Fed-Batch Simultaneous Saccharification and Fermentation of Steam-Exploded Corn Stover |
| title_full | High-Titer L-lactic Acid Production by Fed-Batch Simultaneous Saccharification and Fermentation of Steam-Exploded Corn Stover |
| title_fullStr | High-Titer L-lactic Acid Production by Fed-Batch Simultaneous Saccharification and Fermentation of Steam-Exploded Corn Stover |
| title_full_unstemmed | High-Titer L-lactic Acid Production by Fed-Batch Simultaneous Saccharification and Fermentation of Steam-Exploded Corn Stover |
| title_short | High-Titer L-lactic Acid Production by Fed-Batch Simultaneous Saccharification and Fermentation of Steam-Exploded Corn Stover |
| title_sort | high titer l lactic acid production by fed batch simultaneous saccharification and fermentation of steam exploded corn stover |
| topic | steam explosion L-lactic acid simultaneous saccharification and fermentation fed-batch hydrolysis <i>Bacillus coagulans</i> |
| url | https://www.mdpi.com/2311-5637/11/1/25 |
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