Chia (<i>Salvia hispanica</i>) Seed Oil Modulates the Haemato-Immunological Response, Antioxidative Status and Cytokine Gene Expression of Tropical Freshwater Teleost, <i>Labeo rohita</i>
The present investigation attempts to evaluate the impact of the dietary inclusion of chia (<i>Salvia hispanica</i>) seed oil (CSO) on the indices of haemato-immunology, metabolic enzymes, and expression of immune-responsive cytokine genes in <i>Labeo rohita</i> (rohu) finger...
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2025-01-01
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| author | Sanjay Kumar Gupta Rajan Gupta Akruti Gupta Md Javed Foysal Kishore Kumar Krishnani |
| author_facet | Sanjay Kumar Gupta Rajan Gupta Akruti Gupta Md Javed Foysal Kishore Kumar Krishnani |
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| description | The present investigation attempts to evaluate the impact of the dietary inclusion of chia (<i>Salvia hispanica</i>) seed oil (CSO) on the indices of haemato-immunology, metabolic enzymes, and expression of immune-responsive cytokine genes in <i>Labeo rohita</i> (rohu) fingerlings. The responses were observed in a 60-day feeding trial, set up with a total of 180 rohu fingerlings (19.74 ± 0.33 g) randomly allocated to four treatment groups with three replicates each. The groups were fed with a basal diet incorporated with 0%, 1.0%, 2.0%, and 3.0% CSO, denoted as control, CSO (1), CSO (2), and CSO (3), respectively. Significant (<i>p</i> < 0.05) augmentation of hematological indices such as total protein and globulin levels was observed in the group fed a 1.0% CSO-supplemented diet. Serum glucose, cholesterol, triglycerides, and complement reactive protein levels declined, whereas marker anti-oxidative enzymes (SOD, CAT, and GST) and protein metabolic enzymes (ALT and AST) increased (<i>p</i> < 0.05) in the lowest CSO-supplemented group. A significant upregulation of inflammatory cytokine viz. IL-1β, IFN-γ, TNF-α, and TLR22 alongside downregulation of IL-10 was noted in various tissues. The results support the inclusion of 1.0% CSO as a prospective dietary vegan substitute to fish oil in rohu aquaculture. |
| format | Article |
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| institution | Kabale University |
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| language | English |
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| spelling | doaj-art-affb852227774b54b2a93b7b7f2b29162025-01-24T13:23:38ZengMDPI AGBiology2079-77372025-01-011419510.3390/biology14010095Chia (<i>Salvia hispanica</i>) Seed Oil Modulates the Haemato-Immunological Response, Antioxidative Status and Cytokine Gene Expression of Tropical Freshwater Teleost, <i>Labeo rohita</i>Sanjay Kumar Gupta0Rajan Gupta1Akruti Gupta2Md Javed Foysal3Kishore Kumar Krishnani4ICAR-Indian Institute of Agricultural Biotechnology, Ranchi 834010, IndiaICAR-Indian Institute of Agricultural Biotechnology, Ranchi 834010, IndiaICAR-Indian Institute of Agricultural Biotechnology, Ranchi 834010, IndiaSchool of Molecular and Life Sciences, Curtin University, Bentley, WA 6102, AustraliaICAR-Indian Institute of Agricultural Biotechnology, Ranchi 834010, IndiaThe present investigation attempts to evaluate the impact of the dietary inclusion of chia (<i>Salvia hispanica</i>) seed oil (CSO) on the indices of haemato-immunology, metabolic enzymes, and expression of immune-responsive cytokine genes in <i>Labeo rohita</i> (rohu) fingerlings. The responses were observed in a 60-day feeding trial, set up with a total of 180 rohu fingerlings (19.74 ± 0.33 g) randomly allocated to four treatment groups with three replicates each. The groups were fed with a basal diet incorporated with 0%, 1.0%, 2.0%, and 3.0% CSO, denoted as control, CSO (1), CSO (2), and CSO (3), respectively. Significant (<i>p</i> < 0.05) augmentation of hematological indices such as total protein and globulin levels was observed in the group fed a 1.0% CSO-supplemented diet. Serum glucose, cholesterol, triglycerides, and complement reactive protein levels declined, whereas marker anti-oxidative enzymes (SOD, CAT, and GST) and protein metabolic enzymes (ALT and AST) increased (<i>p</i> < 0.05) in the lowest CSO-supplemented group. A significant upregulation of inflammatory cytokine viz. IL-1β, IFN-γ, TNF-α, and TLR22 alongside downregulation of IL-10 was noted in various tissues. The results support the inclusion of 1.0% CSO as a prospective dietary vegan substitute to fish oil in rohu aquaculture.https://www.mdpi.com/2079-7737/14/1/95chia seed oilhaemato-immunologyenzyme assaycytokine gene<i>L. rohita</i> |
| spellingShingle | Sanjay Kumar Gupta Rajan Gupta Akruti Gupta Md Javed Foysal Kishore Kumar Krishnani Chia (<i>Salvia hispanica</i>) Seed Oil Modulates the Haemato-Immunological Response, Antioxidative Status and Cytokine Gene Expression of Tropical Freshwater Teleost, <i>Labeo rohita</i> Biology chia seed oil haemato-immunology enzyme assay cytokine gene <i>L. rohita</i> |
| title | Chia (<i>Salvia hispanica</i>) Seed Oil Modulates the Haemato-Immunological Response, Antioxidative Status and Cytokine Gene Expression of Tropical Freshwater Teleost, <i>Labeo rohita</i> |
| title_full | Chia (<i>Salvia hispanica</i>) Seed Oil Modulates the Haemato-Immunological Response, Antioxidative Status and Cytokine Gene Expression of Tropical Freshwater Teleost, <i>Labeo rohita</i> |
| title_fullStr | Chia (<i>Salvia hispanica</i>) Seed Oil Modulates the Haemato-Immunological Response, Antioxidative Status and Cytokine Gene Expression of Tropical Freshwater Teleost, <i>Labeo rohita</i> |
| title_full_unstemmed | Chia (<i>Salvia hispanica</i>) Seed Oil Modulates the Haemato-Immunological Response, Antioxidative Status and Cytokine Gene Expression of Tropical Freshwater Teleost, <i>Labeo rohita</i> |
| title_short | Chia (<i>Salvia hispanica</i>) Seed Oil Modulates the Haemato-Immunological Response, Antioxidative Status and Cytokine Gene Expression of Tropical Freshwater Teleost, <i>Labeo rohita</i> |
| title_sort | chia i salvia hispanica i seed oil modulates the haemato immunological response antioxidative status and cytokine gene expression of tropical freshwater teleost i labeo rohita i |
| topic | chia seed oil haemato-immunology enzyme assay cytokine gene <i>L. rohita</i> |
| url | https://www.mdpi.com/2079-7737/14/1/95 |
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