Protective Effect of Daikenchuto on Dextran Sulfate Sodium-Induced Colitis in Mice
Aim. To investigate the effect of daikenchuto (TJ-100; DKT) for ulcerative colitis (UC) model mouse and assess its anti-inflammatory mechanisms. Methods. We evaluated the effects of DKT on dextran sulfate sodium- (DSS-) induced experimental colitis. First, we assessed the short-term effects of DKT u...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2017/1298263 |
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| author | Takaharu Matsunaga Shinichi Hashimoto Naoki Yamamoto Ryo Kawasato Tomohiro Shirasawa Atsushi Goto Koichi Fujisawa Taro Takami Takeshi Okamoto Jun Nishikawa Isao Sakaida |
| author_facet | Takaharu Matsunaga Shinichi Hashimoto Naoki Yamamoto Ryo Kawasato Tomohiro Shirasawa Atsushi Goto Koichi Fujisawa Taro Takami Takeshi Okamoto Jun Nishikawa Isao Sakaida |
| author_sort | Takaharu Matsunaga |
| collection | DOAJ |
| description | Aim. To investigate the effect of daikenchuto (TJ-100; DKT) for ulcerative colitis (UC) model mouse and assess its anti-inflammatory mechanisms. Methods. We evaluated the effects of DKT on dextran sulfate sodium- (DSS-) induced experimental colitis. First, we assessed the short-term effects of DKT using two groups: 5% DSS group and 5% DSS with DKT group. Colon length; histological scores; and interleukin- (IL-) 10, IL-1β, and tumor necrosis factor-α mRNA expression profiles were analyzed using real-time PCR. Second, we assessed the long-term effects of DKT, by comparing survival time between 2% DSS and 2% DSS with DKT groups. Results. After 7 days, the colon lengths of DSS + DKT group were longer than those of the DSS group (mean values: 6.11 versus 5.69 cm, p<0.05). Furthermore, compared to DSS group, the DSS + DKT group maintained significantly higher levels of serum hemoglobin (13.1 versus 10.7 g/dL, p<0.05) and exhibited significantly higher expression levels of IL-10 (p<0.05). The 2% DSS + DKT group exhibited significantly longer survival time than the 2% DSS group (70 versus 44 days, p<0.01). Conclusion. Our results indicate that DKT prevented inflammation in the colon, indicating its potential as a new therapeutic agent for UC. |
| format | Article |
| id | doaj-art-afe9d0cd86fc48b78591ffbc10f89197 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-afe9d0cd86fc48b78591ffbc10f891972025-02-03T01:00:21ZengWileyGastroenterology Research and Practice1687-61211687-630X2017-01-01201710.1155/2017/12982631298263Protective Effect of Daikenchuto on Dextran Sulfate Sodium-Induced Colitis in MiceTakaharu Matsunaga0Shinichi Hashimoto1Naoki Yamamoto2Ryo Kawasato3Tomohiro Shirasawa4Atsushi Goto5Koichi Fujisawa6Taro Takami7Takeshi Okamoto8Jun Nishikawa9Isao Sakaida10Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi 755-8505, JapanAim. To investigate the effect of daikenchuto (TJ-100; DKT) for ulcerative colitis (UC) model mouse and assess its anti-inflammatory mechanisms. Methods. We evaluated the effects of DKT on dextran sulfate sodium- (DSS-) induced experimental colitis. First, we assessed the short-term effects of DKT using two groups: 5% DSS group and 5% DSS with DKT group. Colon length; histological scores; and interleukin- (IL-) 10, IL-1β, and tumor necrosis factor-α mRNA expression profiles were analyzed using real-time PCR. Second, we assessed the long-term effects of DKT, by comparing survival time between 2% DSS and 2% DSS with DKT groups. Results. After 7 days, the colon lengths of DSS + DKT group were longer than those of the DSS group (mean values: 6.11 versus 5.69 cm, p<0.05). Furthermore, compared to DSS group, the DSS + DKT group maintained significantly higher levels of serum hemoglobin (13.1 versus 10.7 g/dL, p<0.05) and exhibited significantly higher expression levels of IL-10 (p<0.05). The 2% DSS + DKT group exhibited significantly longer survival time than the 2% DSS group (70 versus 44 days, p<0.01). Conclusion. Our results indicate that DKT prevented inflammation in the colon, indicating its potential as a new therapeutic agent for UC.http://dx.doi.org/10.1155/2017/1298263 |
| spellingShingle | Takaharu Matsunaga Shinichi Hashimoto Naoki Yamamoto Ryo Kawasato Tomohiro Shirasawa Atsushi Goto Koichi Fujisawa Taro Takami Takeshi Okamoto Jun Nishikawa Isao Sakaida Protective Effect of Daikenchuto on Dextran Sulfate Sodium-Induced Colitis in Mice Gastroenterology Research and Practice |
| title | Protective Effect of Daikenchuto on Dextran Sulfate Sodium-Induced Colitis in Mice |
| title_full | Protective Effect of Daikenchuto on Dextran Sulfate Sodium-Induced Colitis in Mice |
| title_fullStr | Protective Effect of Daikenchuto on Dextran Sulfate Sodium-Induced Colitis in Mice |
| title_full_unstemmed | Protective Effect of Daikenchuto on Dextran Sulfate Sodium-Induced Colitis in Mice |
| title_short | Protective Effect of Daikenchuto on Dextran Sulfate Sodium-Induced Colitis in Mice |
| title_sort | protective effect of daikenchuto on dextran sulfate sodium induced colitis in mice |
| url | http://dx.doi.org/10.1155/2017/1298263 |
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