Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis
Background. Nontuberculous mycobacteria (NTM) are widely present in environments, such as soil and water, and have recently been recognized as important pathogenic bacteria. The incidence of NTM-related infections is steadily increasing. As the diagnosis and treatment of NTM infection should be dist...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/8844306 |
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| author | Min-Jeong Kim Kyu-Min Kim Jeong-Ih Shin Jong-Hun Ha Dong-Hae Lee Jeong-Gyu Choi Jin-Sik Park Jung-Hyun Byun Jung-Wan Yoo Seokyong Eum Myunghwan Jung Seung Chul Baik Woo Kon Lee Hyung Lyun Kang Min-Kyoung Shin |
| author_facet | Min-Jeong Kim Kyu-Min Kim Jeong-Ih Shin Jong-Hun Ha Dong-Hae Lee Jeong-Gyu Choi Jin-Sik Park Jung-Hyun Byun Jung-Wan Yoo Seokyong Eum Myunghwan Jung Seung Chul Baik Woo Kon Lee Hyung Lyun Kang Min-Kyoung Shin |
| author_sort | Min-Jeong Kim |
| collection | DOAJ |
| description | Background. Nontuberculous mycobacteria (NTM) are widely present in environments, such as soil and water, and have recently been recognized as important pathogenic bacteria. The incidence of NTM-related infections is steadily increasing. As the diagnosis and treatment of NTM infection should be distinguished from tuberculosis, and the treatment should be specific to the species of NTM acquired, accurate species identification is required. Methods. In this study, two-step multiplex PCR (mPCR) and multigene sequence-based analysis were used to accurately identify NTM species in 320 clinical isolates from Gyeongsang National University Hospital (GNUH). In particular, major mycobacterial strains with a high isolation frequency as well as coinfections with multiple species were diagnosed through two-step mPCR. Multigene sequencing was performed to accurately identify other NTM species not detected by mPCR. Variable regions of the genes 16S rRNA, rpoB, hsp65, and 16S-23S rRNA internal transcribed spacer were included in the analysis. Results. Two-step mPCR identified 234 (73.1%) cases of M. intracellulare, 26 (8.1%) cases of M. avium subsp. avium, and 13 (4.1%) cases of M. avium subsp. hominissuis infection. Additionally, 9 (2.8%) M. fortuitum, 9 (2.8%) M. massiliense, 2 (0.6%) M. abscessus, and 4 (1.2%) M. kansasii isolates were identified. Coinfection was identified in 7 (2.2%) samples. The sixteen samples not classified by two-step mPCR included 6 (1.9%) cases of M. chimaera, 4 (1.3%) M. gordonae, 1 (0.3%) M. colombiense, 1 (0.3%) M. mageritense, and 1 (0.3%) M. persicum identified by sequence analysis. Conclusions. The results of this study suggest a strategy for rapid detection and accurate identification of species using two-step mPCR and multigene sequence-based analysis. To the best of our knowledge, this study is the first to report the identification of NTM species isolated from patients in Gyeongnam/Korea. |
| format | Article |
| id | doaj-art-afcabb8c207b4b718fc971525419ae65 |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-afcabb8c207b4b718fc971525419ae652025-02-03T00:58:45ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932021-01-01202110.1155/2021/88443068844306Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based AnalysisMin-Jeong Kim0Kyu-Min Kim1Jeong-Ih Shin2Jong-Hun Ha3Dong-Hae Lee4Jeong-Gyu Choi5Jin-Sik Park6Jung-Hyun Byun7Jung-Wan Yoo8Seokyong Eum9Myunghwan Jung10Seung Chul Baik11Woo Kon Lee12Hyung Lyun Kang13Min-Kyoung Shin14Department of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Laboratory Medicine, Gyeongsang National University Hospital, Jinju 52727, Republic of KoreaDepartment of Internal Medicine, Gyeongsang National University Hospital, Jinju 52727, Republic of KoreaInternational Tuberculosis Research Center, Changwon 51755, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaDepartment of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of KoreaBackground. Nontuberculous mycobacteria (NTM) are widely present in environments, such as soil and water, and have recently been recognized as important pathogenic bacteria. The incidence of NTM-related infections is steadily increasing. As the diagnosis and treatment of NTM infection should be distinguished from tuberculosis, and the treatment should be specific to the species of NTM acquired, accurate species identification is required. Methods. In this study, two-step multiplex PCR (mPCR) and multigene sequence-based analysis were used to accurately identify NTM species in 320 clinical isolates from Gyeongsang National University Hospital (GNUH). In particular, major mycobacterial strains with a high isolation frequency as well as coinfections with multiple species were diagnosed through two-step mPCR. Multigene sequencing was performed to accurately identify other NTM species not detected by mPCR. Variable regions of the genes 16S rRNA, rpoB, hsp65, and 16S-23S rRNA internal transcribed spacer were included in the analysis. Results. Two-step mPCR identified 234 (73.1%) cases of M. intracellulare, 26 (8.1%) cases of M. avium subsp. avium, and 13 (4.1%) cases of M. avium subsp. hominissuis infection. Additionally, 9 (2.8%) M. fortuitum, 9 (2.8%) M. massiliense, 2 (0.6%) M. abscessus, and 4 (1.2%) M. kansasii isolates were identified. Coinfection was identified in 7 (2.2%) samples. The sixteen samples not classified by two-step mPCR included 6 (1.9%) cases of M. chimaera, 4 (1.3%) M. gordonae, 1 (0.3%) M. colombiense, 1 (0.3%) M. mageritense, and 1 (0.3%) M. persicum identified by sequence analysis. Conclusions. The results of this study suggest a strategy for rapid detection and accurate identification of species using two-step mPCR and multigene sequence-based analysis. To the best of our knowledge, this study is the first to report the identification of NTM species isolated from patients in Gyeongnam/Korea.http://dx.doi.org/10.1155/2021/8844306 |
| spellingShingle | Min-Jeong Kim Kyu-Min Kim Jeong-Ih Shin Jong-Hun Ha Dong-Hae Lee Jeong-Gyu Choi Jin-Sik Park Jung-Hyun Byun Jung-Wan Yoo Seokyong Eum Myunghwan Jung Seung Chul Baik Woo Kon Lee Hyung Lyun Kang Min-Kyoung Shin Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis |
| title_full | Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis |
| title_fullStr | Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis |
| title_full_unstemmed | Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis |
| title_short | Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis |
| title_sort | identification of nontuberculous mycobacteria in patients with pulmonary diseases in gyeongnam korea using multiplex pcr and multigene sequence based analysis |
| url | http://dx.doi.org/10.1155/2021/8844306 |
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