Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice
Neonatal studies in different mouse strains reveal that early life colonization affects the development of adaptive immunity in mice. The nonobese diabetic (NOD) mouse spontaneously develops autoimmune diabetes, but neonatal studies of NOD mice are lacking. We hypothesized that NOD mice deviate from...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/6321980 |
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| author | Dina Silke Malling Damlund Stine Broeng Metzdorff Jane Preuss Hasselby Maria Wiese Mia Lundsager Dennis Sandris Nielsen Karsten Stig Buschard Axel Kornerup Hansen Hanne Frøkiær |
| author_facet | Dina Silke Malling Damlund Stine Broeng Metzdorff Jane Preuss Hasselby Maria Wiese Mia Lundsager Dennis Sandris Nielsen Karsten Stig Buschard Axel Kornerup Hansen Hanne Frøkiær |
| author_sort | Dina Silke Malling Damlund |
| collection | DOAJ |
| description | Neonatal studies in different mouse strains reveal that early life colonization affects the development of adaptive immunity in mice. The nonobese diabetic (NOD) mouse spontaneously develops autoimmune diabetes, but neonatal studies of NOD mice are lacking. We hypothesized that NOD mice deviate from another much used mouse strain, C57BL/6, with respect to postnatal microbiota and/or hematopoiesis and compared this in newborn mice of dams housed under the same conditions. A distinct bacteria profile rich in staphylococci was found at postnatal days (PND) 1–4 in NOD mice. Furthermore, a distinct splenic cell profile high in a granulocytic phenotype was evident in the neonatal NOD mice whereas neonatal C57BL/6 mice showed a profile rich in monocytes. Neonatal expression of Reg3g and Muc2 in the gut was deviating in NOD mice and coincided with fewer bacteria attaching to the Mucosal surface in NOD compared to C57BL/6 mice. |
| format | Article |
| id | doaj-art-afc9ee67a0b74f4ebf7cd4e600685151 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-afc9ee67a0b74f4ebf7cd4e6006851512025-02-03T01:27:54ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/63219806321980Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 MiceDina Silke Malling Damlund0Stine Broeng Metzdorff1Jane Preuss Hasselby2Maria Wiese3Mia Lundsager4Dennis Sandris Nielsen5Karsten Stig Buschard6Axel Kornerup Hansen7Hanne Frøkiær8Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Pathology, Rigshospitalet, 2100 Copenhagen, DenmarkDepartment of Food Science, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Food Science, University of Copenhagen, 1870 Frederiksberg C, DenmarkBartholin Institute, Rigshospitalet, 2100 Copenhagen, DenmarkDepartment of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkDepartment of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, DenmarkNeonatal studies in different mouse strains reveal that early life colonization affects the development of adaptive immunity in mice. The nonobese diabetic (NOD) mouse spontaneously develops autoimmune diabetes, but neonatal studies of NOD mice are lacking. We hypothesized that NOD mice deviate from another much used mouse strain, C57BL/6, with respect to postnatal microbiota and/or hematopoiesis and compared this in newborn mice of dams housed under the same conditions. A distinct bacteria profile rich in staphylococci was found at postnatal days (PND) 1–4 in NOD mice. Furthermore, a distinct splenic cell profile high in a granulocytic phenotype was evident in the neonatal NOD mice whereas neonatal C57BL/6 mice showed a profile rich in monocytes. Neonatal expression of Reg3g and Muc2 in the gut was deviating in NOD mice and coincided with fewer bacteria attaching to the Mucosal surface in NOD compared to C57BL/6 mice.http://dx.doi.org/10.1155/2016/6321980 |
| spellingShingle | Dina Silke Malling Damlund Stine Broeng Metzdorff Jane Preuss Hasselby Maria Wiese Mia Lundsager Dennis Sandris Nielsen Karsten Stig Buschard Axel Kornerup Hansen Hanne Frøkiær Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice Journal of Diabetes Research |
| title | Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice |
| title_full | Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice |
| title_fullStr | Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice |
| title_full_unstemmed | Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice |
| title_short | Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice |
| title_sort | postnatal hematopoiesis and gut microbiota in nod mice deviate from c57bl 6 mice |
| url | http://dx.doi.org/10.1155/2016/6321980 |
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