Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in rice
Abstract Chloroplasts play a vital role in plant growth and development, which are the main sites of photosynthesis and the production of hormones and metabolites. Despite their significance, the regulatory mechanisms governing chloroplast development remain unclear. In our investigation, we identif...
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| Format: | Article |
| Language: | English |
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Wiley
2024-06-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.610 |
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| author | Xiaoqiong Guo Chunli Wang Qian Zhu Wenhua Dongchen Xiaoling Zhang Wei Li Hui Zhang Cui Zhang Zar Ni Naing Nant Nyein Mengting Li Lijuan Chen Dongsun Lee |
| author_facet | Xiaoqiong Guo Chunli Wang Qian Zhu Wenhua Dongchen Xiaoling Zhang Wei Li Hui Zhang Cui Zhang Zar Ni Naing Nant Nyein Mengting Li Lijuan Chen Dongsun Lee |
| author_sort | Xiaoqiong Guo |
| collection | DOAJ |
| description | Abstract Chloroplasts play a vital role in plant growth and development, which are the main sites of photosynthesis and the production of hormones and metabolites. Despite their significance, the regulatory mechanisms governing chloroplast development remain unclear. In our investigation, we identified a rice mutant with defective chloroplasts in rice (Oryza sativa L.), named albino lethal 13 (osal13), which displayed a distinct albino phenotype in leaves, ultimately resulting in seedling lethality. Molecular cloning revealed that OsAL13 encodes a novel rice protein with no homologous gene or known conserved domain. This gene was located in the chloroplast and exhibited constitutive expression in various tissues, particularly in green tissues and regions of active cell growth. Our study's findings reveal that RNAi‐mediated knockdown of OsAL13 led to a pronounced albino phenotype, reduced chlorophyll and carotenoid contents, a vesicle chloroplast structure, and a decrease in the expression of chloroplast‐associated genes. Consequently, the pollen fertility and seed setting rate were lower compared with the wild type. In contrast, the overexpression of OsAL13 resulted in an increased photosynthetic rate, a higher total grain number per panicle, and enhanced levels of indole‐3‐acetic acid (IAA) in the roots and gibberellin A3 (GA3) in the shoot. These outcomes provide new insights on the role of OsAL13 in regulating chloroplast development in rice. |
| format | Article |
| id | doaj-art-afc5c0abcdc04871aaf4e987e507bef9 |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-afc5c0abcdc04871aaf4e987e507bef92025-02-04T08:35:53ZengWileyPlant Direct2475-44552024-06-0186n/an/a10.1002/pld3.610Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in riceXiaoqiong Guo0Chunli Wang1Qian Zhu2Wenhua Dongchen3Xiaoling Zhang4Wei Li5Hui Zhang6Cui Zhang7Zar Ni Naing Nant Nyein8Mengting Li9Lijuan Chen10Dongsun Lee11Rice Research Institute Yunnan Agricultural University Kunming ChinaRice Research Institute Yunnan Agricultural University Kunming ChinaRice Research Institute Yunnan Agricultural University Kunming ChinaCollege of Agronomy and Biotechnology Yunnan Agricultural University Kunming ChinaKunming University Kunming ChinaCollege of Biological Resource and Food Engineering Qujing Normal University Qujing ChinaCollege of Agronomy and Biotechnology Yunnan Agricultural University Kunming ChinaRice Research Institute Yunnan Agricultural University Kunming ChinaRice Research Institute Yunnan Agricultural University Kunming ChinaRice Research Institute Yunnan Agricultural University Kunming ChinaRice Research Institute Yunnan Agricultural University Kunming ChinaRice Research Institute Yunnan Agricultural University Kunming ChinaAbstract Chloroplasts play a vital role in plant growth and development, which are the main sites of photosynthesis and the production of hormones and metabolites. Despite their significance, the regulatory mechanisms governing chloroplast development remain unclear. In our investigation, we identified a rice mutant with defective chloroplasts in rice (Oryza sativa L.), named albino lethal 13 (osal13), which displayed a distinct albino phenotype in leaves, ultimately resulting in seedling lethality. Molecular cloning revealed that OsAL13 encodes a novel rice protein with no homologous gene or known conserved domain. This gene was located in the chloroplast and exhibited constitutive expression in various tissues, particularly in green tissues and regions of active cell growth. Our study's findings reveal that RNAi‐mediated knockdown of OsAL13 led to a pronounced albino phenotype, reduced chlorophyll and carotenoid contents, a vesicle chloroplast structure, and a decrease in the expression of chloroplast‐associated genes. Consequently, the pollen fertility and seed setting rate were lower compared with the wild type. In contrast, the overexpression of OsAL13 resulted in an increased photosynthetic rate, a higher total grain number per panicle, and enhanced levels of indole‐3‐acetic acid (IAA) in the roots and gibberellin A3 (GA3) in the shoot. These outcomes provide new insights on the role of OsAL13 in regulating chloroplast development in rice.https://doi.org/10.1002/pld3.610albino genechloroplast developmentOsAL13rice (Oryza sativa L.) |
| spellingShingle | Xiaoqiong Guo Chunli Wang Qian Zhu Wenhua Dongchen Xiaoling Zhang Wei Li Hui Zhang Cui Zhang Zar Ni Naing Nant Nyein Mengting Li Lijuan Chen Dongsun Lee Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in rice Plant Direct albino gene chloroplast development OsAL13 rice (Oryza sativa L.) |
| title | Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in rice |
| title_full | Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in rice |
| title_fullStr | Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in rice |
| title_full_unstemmed | Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in rice |
| title_short | Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in rice |
| title_sort | albino lethal 13 a chloroplast imported protein required for chloroplast development in rice |
| topic | albino gene chloroplast development OsAL13 rice (Oryza sativa L.) |
| url | https://doi.org/10.1002/pld3.610 |
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