A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways
<i>SMALL AUXIN UP-REGULATED RNA</i> (<i>SAURs</i>) genes are acknowledged as auxin-responsive genes that play crucial roles in modulating adaptive growth under abiotic stress conditions. Low temperatures constitute a primary limiting factor that significantly impairs the deve...
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2025-01-01
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| author | Duo Wang Gangli Ma Jia Shen Xinyang Xu Weisong Shou Zhengying Xuan Yanjun He |
| author_facet | Duo Wang Gangli Ma Jia Shen Xinyang Xu Weisong Shou Zhengying Xuan Yanjun He |
| author_sort | Duo Wang |
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| description | <i>SMALL AUXIN UP-REGULATED RNA</i> (<i>SAURs</i>) genes are acknowledged as auxin-responsive genes that play crucial roles in modulating adaptive growth under abiotic stress conditions. Low temperatures constitute a primary limiting factor that significantly impairs the development, growth, and fruit quality of watermelon plants during the winter and spring seasons. Despite their potential importance, <i>SAURs</i> have not yet been thoroughly investigated or characterized in watermelon. In this study, we identified a positive regulator of the chilling stress response among watermelon <i>SAURs,</i> designated as <i>ClSAUR1</i>. Subcellular localization analysis demonstrated that the protein is directed to both the nucleus and cytoplasm. Quantitative real-time PCR (qRT-PCR) analysis indicated that <i>ClSAUR1</i> is ubiquitously expressed across various watermelon tissues, with pronounced expression in the roots and leaves. Moreover, qRT-PCR and promoter::β-glucuronidase (<i>GUS</i>) staining assays revealed that the expression of <i>ClSAUR1</i> is significantly upregulated in response to exogenous abscisic acid (ABA) and chilling stress. The overexpression of <i>ClSAUR1</i> in tobacco lines was contrasted and analyzed, revealing an increased tolerance to chilling stress. This was evidenced by a reduced degree of wilting and chlorosis compared to wild-type (WT) plants. Furthermore, the overexpressed lines showed reduced reactive oxygen species (ROS) accumulation and increased antioxidant enzyme activity. The qRT-PCR results further indicated that the expression levels of genes associated with abscisic acid (ABA), antioxidant enzymes, and CBF–COR cold-responsive pathways were upregulated in the transgenic tobacco lines. This study provides new insights into the role of <i>ClSAURs</i> in enhancing the cold resistance of watermelon. |
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| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj-art-afb6754648a64f2c815e76b7ec2f41f32025-01-24T13:34:37ZengMDPI AGHorticulturae2311-75242025-01-011115210.3390/horticulturae11010052A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling PathwaysDuo Wang0Gangli Ma1Jia Shen2Xinyang Xu3Weisong Shou4Zhengying Xuan5Yanjun He6Zhejiang Academy of Agricultural Sciences, Institute of Vegetables, Hangzhou 310021, ChinaZhejiang Academy of Agricultural Sciences, Institute of Vegetables, Hangzhou 310021, ChinaZhejiang Academy of Agricultural Sciences, Institute of Vegetables, Hangzhou 310021, ChinaZhejiang Academy of Agricultural Sciences, Institute of Vegetables, Hangzhou 310021, ChinaZhejiang Academy of Agricultural Sciences, Institute of Vegetables, Hangzhou 310021, ChinaKey Laboratory of Protected Agriculture, College of Horticulture and Forestry, Tarim University, Alar 843300, ChinaZhejiang Academy of Agricultural Sciences, Institute of Vegetables, Hangzhou 310021, China<i>SMALL AUXIN UP-REGULATED RNA</i> (<i>SAURs</i>) genes are acknowledged as auxin-responsive genes that play crucial roles in modulating adaptive growth under abiotic stress conditions. Low temperatures constitute a primary limiting factor that significantly impairs the development, growth, and fruit quality of watermelon plants during the winter and spring seasons. Despite their potential importance, <i>SAURs</i> have not yet been thoroughly investigated or characterized in watermelon. In this study, we identified a positive regulator of the chilling stress response among watermelon <i>SAURs,</i> designated as <i>ClSAUR1</i>. Subcellular localization analysis demonstrated that the protein is directed to both the nucleus and cytoplasm. Quantitative real-time PCR (qRT-PCR) analysis indicated that <i>ClSAUR1</i> is ubiquitously expressed across various watermelon tissues, with pronounced expression in the roots and leaves. Moreover, qRT-PCR and promoter::β-glucuronidase (<i>GUS</i>) staining assays revealed that the expression of <i>ClSAUR1</i> is significantly upregulated in response to exogenous abscisic acid (ABA) and chilling stress. The overexpression of <i>ClSAUR1</i> in tobacco lines was contrasted and analyzed, revealing an increased tolerance to chilling stress. This was evidenced by a reduced degree of wilting and chlorosis compared to wild-type (WT) plants. Furthermore, the overexpressed lines showed reduced reactive oxygen species (ROS) accumulation and increased antioxidant enzyme activity. The qRT-PCR results further indicated that the expression levels of genes associated with abscisic acid (ABA), antioxidant enzymes, and CBF–COR cold-responsive pathways were upregulated in the transgenic tobacco lines. This study provides new insights into the role of <i>ClSAURs</i> in enhancing the cold resistance of watermelon.https://www.mdpi.com/2311-7524/11/1/52<i>ClSAUR1</i>Watermelon (<i>Citrullus lanatus</i>)chilling stressABAROStobacco |
| spellingShingle | Duo Wang Gangli Ma Jia Shen Xinyang Xu Weisong Shou Zhengying Xuan Yanjun He A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways Horticulturae <i>ClSAUR1</i> Watermelon (<i>Citrullus lanatus</i>) chilling stress ABA ROS tobacco |
| title | A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways |
| title_full | A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways |
| title_fullStr | A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways |
| title_full_unstemmed | A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways |
| title_short | A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways |
| title_sort | i small auxin up regulated rna i gene isolated from watermelon i clsaur1 i positively modulates the chilling stress response in tobacco via multiple signaling pathways |
| topic | <i>ClSAUR1</i> Watermelon (<i>Citrullus lanatus</i>) chilling stress ABA ROS tobacco |
| url | https://www.mdpi.com/2311-7524/11/1/52 |
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