Acidosis activates complement system in vitro
We investigated the in vitro effect of different form s of acidosis (pH 7.0) on the formation of anaphylatoxins C3a and C5a. Metabolic acidosis due to addition of hydrochloric acid (10 μ mol/ml blood) or lactic acid (5.5 μ mol/ml) to heparin blood (N=12) caused significant activation of C3a and C5a...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359890802 |
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| _version_ | 1832567188909719552 |
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| author | Michael Emeis Josef Sonntag Carsten Willam Evelyn Strauss Matthias M. Walka Michael Obladen |
| author_facet | Michael Emeis Josef Sonntag Carsten Willam Evelyn Strauss Matthias M. Walka Michael Obladen |
| author_sort | Michael Emeis |
| collection | DOAJ |
| description | We investigated the in vitro effect of different form s of acidosis (pH 7.0) on the formation of anaphylatoxins C3a and C5a. Metabolic acidosis due to addition of hydrochloric acid (10 μ mol/ml blood) or lactic acid (5.5 μ mol/ml) to heparin blood (N=12) caused significant activation of C3a and C5a compared to control (both p=0.002). Respiratory acidosis activated C3a (p=0.007) and C5a (p=0.003) compared to normocapnic controls. Making blood samples with lactic acidosis hypocapnic resulted in a median pH of 7.37. In this respiratory compensated metabolic acidosis, C3a and C5a were not increased. These experiments show that acidosis itself and not lactate trigger for activation of complement components C3 and C5. |
| format | Article |
| id | doaj-art-afb32647cf35447aa72de8d4c4dc4ca8 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-afb32647cf35447aa72de8d4c4dc4ca82025-02-03T01:02:10ZengWileyMediators of Inflammation0962-93511466-18611998-01-017641742010.1080/09629359890802Acidosis activates complement system in vitroMichael Emeis0Josef Sonntag1Carsten Willam2Evelyn Strauss3Matthias M. Walka4Michael Obladen5Department of Neonatology, Charité, Virchow-Hospital, Humboldt-University Berlin, Berlin 13353, GermanyDepartment of Neonatology, Charité, Virchow-Hospital, Humboldt-University Berlin, Berlin 13353, GermanyDepartment of Nephrology, Charité, Virchow-Hospital, Humboldt-University Berlin, Berlin 13353, GermanyDepartment of Neonatology, Charité, Virchow-Hospital, Humboldt-University Berlin, Berlin 13353, GermanyDepartment of Neonatology, Charité, Virchow-Hospital, Humboldt-University Berlin, Berlin 13353, GermanyDepartment of Neonatology, Charité, Virchow-Hospital, Humboldt-University Berlin, Berlin 13353, GermanyWe investigated the in vitro effect of different form s of acidosis (pH 7.0) on the formation of anaphylatoxins C3a and C5a. Metabolic acidosis due to addition of hydrochloric acid (10 μ mol/ml blood) or lactic acid (5.5 μ mol/ml) to heparin blood (N=12) caused significant activation of C3a and C5a compared to control (both p=0.002). Respiratory acidosis activated C3a (p=0.007) and C5a (p=0.003) compared to normocapnic controls. Making blood samples with lactic acidosis hypocapnic resulted in a median pH of 7.37. In this respiratory compensated metabolic acidosis, C3a and C5a were not increased. These experiments show that acidosis itself and not lactate trigger for activation of complement components C3 and C5.http://dx.doi.org/10.1080/09629359890802AcidosisAlcalosisComplement systemComplement activationAnaphylatoxins. |
| spellingShingle | Michael Emeis Josef Sonntag Carsten Willam Evelyn Strauss Matthias M. Walka Michael Obladen Acidosis activates complement system in vitro Mediators of Inflammation Acidosis Alcalosis Complement system Complement activation Anaphylatoxins. |
| title | Acidosis activates complement system in vitro |
| title_full | Acidosis activates complement system in vitro |
| title_fullStr | Acidosis activates complement system in vitro |
| title_full_unstemmed | Acidosis activates complement system in vitro |
| title_short | Acidosis activates complement system in vitro |
| title_sort | acidosis activates complement system in vitro |
| topic | Acidosis Alcalosis Complement system Complement activation Anaphylatoxins. |
| url | http://dx.doi.org/10.1080/09629359890802 |
| work_keys_str_mv | AT michaelemeis acidosisactivatescomplementsysteminvitro AT josefsonntag acidosisactivatescomplementsysteminvitro AT carstenwillam acidosisactivatescomplementsysteminvitro AT evelynstrauss acidosisactivatescomplementsysteminvitro AT matthiasmwalka acidosisactivatescomplementsysteminvitro AT michaelobladen acidosisactivatescomplementsysteminvitro |