HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora
Phyla nodiflora is a creeping perennial herb, widely distributed in the most tropical and subtropical regions. It has been used as a folk medicine, herbal beverage, or folk cosmetic. For these usages, the development of a chemical quality control method of this plant is necessary. In the present stu...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/528653 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563063095558144 |
|---|---|
| author | Fang-Ju Lin Feng-Lin Yen Pei-Chun Chen Moo-Chin Wang Chun-Nan Lin Chiang-Wen Lee Horng-Huey Ko |
| author_facet | Fang-Ju Lin Feng-Lin Yen Pei-Chun Chen Moo-Chin Wang Chun-Nan Lin Chiang-Wen Lee Horng-Huey Ko |
| author_sort | Fang-Ju Lin |
| collection | DOAJ |
| description | Phyla nodiflora is a creeping perennial herb, widely distributed in the most tropical and subtropical regions. It has been used as a folk medicine, herbal beverage, or folk cosmetic. For these usages, the development of a chemical quality control method of this plant is necessary. In the present study, ten compounds, namely, 3,7,4′,5′-tetrahydroxy-3′-methoxyflavone (1), nodifloretin (2), 4′-hydroxywogonin (3), onopordin (4), cirsiliol (5), 5,7,8,4′-tetrahydroxy-3′-methoxyflavone (6), eupafolin (7), hispidulin (8), larycitrin (9), and β-sitosterol were isolated from the methanolic extract of the aerial part of P. nodiflora (PNM) and their structures were identified by 1D-NMR comparing their spectra with the literature. The antioxidant activities of these compounds were evaluated by free radical scavenging activity and tyrosinase inhibitory effect in cell-free systems. Compounds 4, 5, and 7 showed strong antioxidant activity. To control the quality of P. nodiflora, a simple and reliable method of high-performance liquid chromatography combined with ultraviolet detector (HPLC-UV) was established for both the fingerprint analysis and the quantitative determination of two selected active compounds, onopordin (4) and eupafolin (7). Statistical analysis of the obtained data demonstrated that our method achieved the desired linearity, precision, and accuracy. The results indicated that the developed method can be used as a quality evaluation method for PNM. |
| format | Article |
| id | doaj-art-afa1156d029b43a08cc959979c1d1714 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-afa1156d029b43a08cc959979c1d17142025-02-03T01:21:06ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/528653528653HPLC-Fingerprints and Antioxidant Constituents of Phyla nodifloraFang-Ju Lin0Feng-Lin Yen1Pei-Chun Chen2Moo-Chin Wang3Chun-Nan Lin4Chiang-Wen Lee5Horng-Huey Ko6Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, TaiwanGraduate School of Nursing, Division of Basic Medical Sciences and Chronic Disease and Health Promotion Research Center, Chang Gung University of Science and Technology, Chiayi 613, TaiwanDepartment of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, TaiwanPhyla nodiflora is a creeping perennial herb, widely distributed in the most tropical and subtropical regions. It has been used as a folk medicine, herbal beverage, or folk cosmetic. For these usages, the development of a chemical quality control method of this plant is necessary. In the present study, ten compounds, namely, 3,7,4′,5′-tetrahydroxy-3′-methoxyflavone (1), nodifloretin (2), 4′-hydroxywogonin (3), onopordin (4), cirsiliol (5), 5,7,8,4′-tetrahydroxy-3′-methoxyflavone (6), eupafolin (7), hispidulin (8), larycitrin (9), and β-sitosterol were isolated from the methanolic extract of the aerial part of P. nodiflora (PNM) and their structures were identified by 1D-NMR comparing their spectra with the literature. The antioxidant activities of these compounds were evaluated by free radical scavenging activity and tyrosinase inhibitory effect in cell-free systems. Compounds 4, 5, and 7 showed strong antioxidant activity. To control the quality of P. nodiflora, a simple and reliable method of high-performance liquid chromatography combined with ultraviolet detector (HPLC-UV) was established for both the fingerprint analysis and the quantitative determination of two selected active compounds, onopordin (4) and eupafolin (7). Statistical analysis of the obtained data demonstrated that our method achieved the desired linearity, precision, and accuracy. The results indicated that the developed method can be used as a quality evaluation method for PNM.http://dx.doi.org/10.1155/2014/528653 |
| spellingShingle | Fang-Ju Lin Feng-Lin Yen Pei-Chun Chen Moo-Chin Wang Chun-Nan Lin Chiang-Wen Lee Horng-Huey Ko HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora The Scientific World Journal |
| title | HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora |
| title_full | HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora |
| title_fullStr | HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora |
| title_full_unstemmed | HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora |
| title_short | HPLC-Fingerprints and Antioxidant Constituents of Phyla nodiflora |
| title_sort | hplc fingerprints and antioxidant constituents of phyla nodiflora |
| url | http://dx.doi.org/10.1155/2014/528653 |
| work_keys_str_mv | AT fangjulin hplcfingerprintsandantioxidantconstituentsofphylanodiflora AT fenglinyen hplcfingerprintsandantioxidantconstituentsofphylanodiflora AT peichunchen hplcfingerprintsandantioxidantconstituentsofphylanodiflora AT moochinwang hplcfingerprintsandantioxidantconstituentsofphylanodiflora AT chunnanlin hplcfingerprintsandantioxidantconstituentsofphylanodiflora AT chiangwenlee hplcfingerprintsandantioxidantconstituentsofphylanodiflora AT hornghueyko hplcfingerprintsandantioxidantconstituentsofphylanodiflora |