Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR Method
In the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 µM (nontoxic) and 20 µM (toxic) chromium (VI) concentrations were tested in the m...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2004-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2004.136 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849397147377598464 |
|---|---|
| author | Tamar Kartvelishvili Marina Abuladze Nino Asatiani Joseph Akhvlediani Eugene Kiziria Lali Asanishvili Lia Lejava Hoi-Ying N. Holman Nelly Sapojnikova |
| author_facet | Tamar Kartvelishvili Marina Abuladze Nino Asatiani Joseph Akhvlediani Eugene Kiziria Lali Asanishvili Lia Lejava Hoi-Ying N. Holman Nelly Sapojnikova |
| author_sort | Tamar Kartvelishvili |
| collection | DOAJ |
| description | In the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 µM (nontoxic) and 20 µM (toxic) chromium (VI) concentrations were tested in the model Fenton system with and without catalase-inhibitor sodium azide. The presented approach using the ESR technique along with inhibitors lets us discern cell extract defense capacity connected with the enzymatic activity in viable cells and the catabolic activity in dying cells. |
| format | Article |
| id | doaj-art-af6fcbb711f44ad593c871b793602bd1 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-af6fcbb711f44ad593c871b793602bd12025-08-20T03:39:06ZengWileyThe Scientific World Journal1537-744X2004-01-01478579410.1100/tsw.2004.136Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR MethodTamar Kartvelishvili0Marina Abuladze1Nino Asatiani2Joseph Akhvlediani3Eugene Kiziria4Lali Asanishvili5Lia Lejava6Hoi-Ying N. Holman7Nelly Sapojnikova8Institute of Molecular Biology and Biophysics, Georgian Academy of Sciences, 14, Gotua Str., 0160 Tbilisi, GeorgiaInstitute of Physics, Georgian Academy of Sciences, 6, Tamarashvili Str., 0177 Tbilisi, GeorgiaInstitute of Physics, Georgian Academy of Sciences, 6, Tamarashvili Str., 0177 Tbilisi, GeorgiaInstitute of Physics, Georgian Academy of Sciences, 6, Tamarashvili Str., 0177 Tbilisi, GeorgiaInstitute of Physics, Georgian Academy of Sciences, 6, Tamarashvili Str., 0177 Tbilisi, GeorgiaInstitute of Physics, Georgian Academy of Sciences, 6, Tamarashvili Str., 0177 Tbilisi, GeorgiaInstitute of Physics, Georgian Academy of Sciences, 6, Tamarashvili Str., 0177 Tbilisi, GeorgiaCenter for Environmental Biotechnology, E.O. Lawrence Berkeley National Laboratory, 1, Cyclotron Road, Berkeley, CA, 94720, USAInstitute of Physics, Georgian Academy of Sciences, 6, Tamarashvili Str., 0177 Tbilisi, GeorgiaIn the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 µM (nontoxic) and 20 µM (toxic) chromium (VI) concentrations were tested in the model Fenton system with and without catalase-inhibitor sodium azide. The presented approach using the ESR technique along with inhibitors lets us discern cell extract defense capacity connected with the enzymatic activity in viable cells and the catabolic activity in dying cells.http://dx.doi.org/10.1100/tsw.2004.136 |
| spellingShingle | Tamar Kartvelishvili Marina Abuladze Nino Asatiani Joseph Akhvlediani Eugene Kiziria Lali Asanishvili Lia Lejava Hoi-Ying N. Holman Nelly Sapojnikova Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR Method The Scientific World Journal |
| title | Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR Method |
| title_full | Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR Method |
| title_fullStr | Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR Method |
| title_full_unstemmed | Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR Method |
| title_short | Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR Method |
| title_sort | estimation of the cellular antioxidant response to chromium action using esr method |
| url | http://dx.doi.org/10.1100/tsw.2004.136 |
| work_keys_str_mv | AT tamarkartvelishvili estimationofthecellularantioxidantresponsetochromiumactionusingesrmethod AT marinaabuladze estimationofthecellularantioxidantresponsetochromiumactionusingesrmethod AT ninoasatiani estimationofthecellularantioxidantresponsetochromiumactionusingesrmethod AT josephakhvlediani estimationofthecellularantioxidantresponsetochromiumactionusingesrmethod AT eugenekiziria estimationofthecellularantioxidantresponsetochromiumactionusingesrmethod AT laliasanishvili estimationofthecellularantioxidantresponsetochromiumactionusingesrmethod AT lialejava estimationofthecellularantioxidantresponsetochromiumactionusingesrmethod AT hoiyingnholman estimationofthecellularantioxidantresponsetochromiumactionusingesrmethod AT nellysapojnikova estimationofthecellularantioxidantresponsetochromiumactionusingesrmethod |