THE MORPHOGENIC POTENTIAL OF SIBERIAN POTATO CULTIVARS IN TISSUE CULTURES
Potato is an important crop widely cultivated throughout the world. It is prone to several pathogenic fungi, viruses, and bacteria, which cause severe economic loss every year. Recent advances in plant biotechnology have made it possible to produce resistant cultivars by plant genome editing. This...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2018-05-01
|
| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/1509 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832575180540477440 |
|---|---|
| author | S. M. Ibragimova A. V. Romanova G. Kh. Myzgina A. V. Kochetov |
| author_facet | S. M. Ibragimova A. V. Romanova G. Kh. Myzgina A. V. Kochetov |
| author_sort | S. M. Ibragimova |
| collection | DOAJ |
| description | Potato is an important crop widely cultivated throughout the world. It is prone to several pathogenic fungi, viruses, and bacteria, which cause severe economic loss every year. Recent advances in plant biotechnology have made it possible to produce resistant cultivars by plant genome editing. This approach allows crop modiications that would be diicult to obtain by conventional breeding techniques. A successful and reproducible plant system requires a responsive in vitro regeneration system. Three Siberian potato cultivars –Kemerovchanin, Tuleevsky, and Sapho – and two control cultivars – Golubizna and Nikulinsky – were chosen for in vitro response tests. The stem explants were excised from in vitro grown plantlets. Their stem explants were incubated on Murashige&Skoog (MS) medium supplemented with 1 mg/L transzeatin, 0.1 mg/L IAA, and 10 mg/L GA3 with vitamins (medium P1). All the stages of cultivation up to obtaining fullledged regenerant plants were carried out on medium P1. Every two weeks the explants were transferred to fresh nutrient media. All cultivars formed calluses but difered in terms of callus formation and in callus type. With further cultivation on the medium of the same composition, morphogenesis was observed: shoots formed on wound surfaces of the stem explants. Cultivars Tuleevsky, Kemerovchanin, and Golubizna showed high regeneration ability (73–97.7 %), while that of Sapho (63 %) was poor. All the Siberian cultivars were variable with regard to their morphogenic potentials. Root formation was observed in the shoots within 7 days on hormonefree MS. In the control (hormonefree medium), stem explants of all cultivars did not form calluses or develop shoots on the wound surfaces. The protocol described here is simple and eicient. It can be applied to other potato cultivars. |
| format | Article |
| id | doaj-art-af6e439e350d4ed0b3d3e357c0b9a7ed |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2018-05-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-af6e439e350d4ed0b3d3e357c0b9a7ed2025-02-01T09:58:05ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592018-05-0122331632010.18699/VJ18.366758THE MORPHOGENIC POTENTIAL OF SIBERIAN POTATO CULTIVARS IN TISSUE CULTURESS. M. Ibragimova0A. V. Romanova1G. Kh. Myzgina2A. V. Kochetov3Institute of Cytology and Genetics SB RASInstitute of Cytology and Genetics SB RASSiberian Research Institute of Plant Production and Breeding – Branch of the Institute of Cytology and Genetics SB RASInstitute of Cytology and Genetics SB RAS; Siberian Research Institute of Plant Production and Breeding – Branch of the Institute of Cytology and Genetics SB RAS; Novosibirsk State University; Novosibirsk State Agrarian UniversityPotato is an important crop widely cultivated throughout the world. It is prone to several pathogenic fungi, viruses, and bacteria, which cause severe economic loss every year. Recent advances in plant biotechnology have made it possible to produce resistant cultivars by plant genome editing. This approach allows crop modiications that would be diicult to obtain by conventional breeding techniques. A successful and reproducible plant system requires a responsive in vitro regeneration system. Three Siberian potato cultivars –Kemerovchanin, Tuleevsky, and Sapho – and two control cultivars – Golubizna and Nikulinsky – were chosen for in vitro response tests. The stem explants were excised from in vitro grown plantlets. Their stem explants were incubated on Murashige&Skoog (MS) medium supplemented with 1 mg/L transzeatin, 0.1 mg/L IAA, and 10 mg/L GA3 with vitamins (medium P1). All the stages of cultivation up to obtaining fullledged regenerant plants were carried out on medium P1. Every two weeks the explants were transferred to fresh nutrient media. All cultivars formed calluses but difered in terms of callus formation and in callus type. With further cultivation on the medium of the same composition, morphogenesis was observed: shoots formed on wound surfaces of the stem explants. Cultivars Tuleevsky, Kemerovchanin, and Golubizna showed high regeneration ability (73–97.7 %), while that of Sapho (63 %) was poor. All the Siberian cultivars were variable with regard to their morphogenic potentials. Root formation was observed in the shoots within 7 days on hormonefree MS. In the control (hormonefree medium), stem explants of all cultivars did not form calluses or develop shoots on the wound surfaces. The protocol described here is simple and eicient. It can be applied to other potato cultivars.https://vavilov.elpub.ru/jour/article/view/1509potatosolanum tuberosum l.cultivarsstem explantsregeneration in vitrobiotechnologybreeding |
| spellingShingle | S. M. Ibragimova A. V. Romanova G. Kh. Myzgina A. V. Kochetov THE MORPHOGENIC POTENTIAL OF SIBERIAN POTATO CULTIVARS IN TISSUE CULTURES Вавиловский журнал генетики и селекции potato solanum tuberosum l. cultivars stem explants regeneration in vitro biotechnology breeding |
| title | THE MORPHOGENIC POTENTIAL OF SIBERIAN POTATO CULTIVARS IN TISSUE CULTURES |
| title_full | THE MORPHOGENIC POTENTIAL OF SIBERIAN POTATO CULTIVARS IN TISSUE CULTURES |
| title_fullStr | THE MORPHOGENIC POTENTIAL OF SIBERIAN POTATO CULTIVARS IN TISSUE CULTURES |
| title_full_unstemmed | THE MORPHOGENIC POTENTIAL OF SIBERIAN POTATO CULTIVARS IN TISSUE CULTURES |
| title_short | THE MORPHOGENIC POTENTIAL OF SIBERIAN POTATO CULTIVARS IN TISSUE CULTURES |
| title_sort | morphogenic potential of siberian potato cultivars in tissue cultures |
| topic | potato solanum tuberosum l. cultivars stem explants regeneration in vitro biotechnology breeding |
| url | https://vavilov.elpub.ru/jour/article/view/1509 |
| work_keys_str_mv | AT smibragimova themorphogenicpotentialofsiberianpotatocultivarsintissuecultures AT avromanova themorphogenicpotentialofsiberianpotatocultivarsintissuecultures AT gkhmyzgina themorphogenicpotentialofsiberianpotatocultivarsintissuecultures AT avkochetov themorphogenicpotentialofsiberianpotatocultivarsintissuecultures AT smibragimova morphogenicpotentialofsiberianpotatocultivarsintissuecultures AT avromanova morphogenicpotentialofsiberianpotatocultivarsintissuecultures AT gkhmyzgina morphogenicpotentialofsiberianpotatocultivarsintissuecultures AT avkochetov morphogenicpotentialofsiberianpotatocultivarsintissuecultures |