Remodeling of Mitochondria–Endoplasmic Reticulum Contact Sites Accompanies LUHMES Differentiation
Neural progenitor cells (NPCs) are often used to study the subcellular mechanisms underlying differentiation into neurons in vitro. Works published to date have focused on the pathways that distinguish undifferentiated NPCs from mature neurons, neglecting the earlier and intermediate stages of this...
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2025-01-01
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| Online Access: | https://www.mdpi.com/2218-273X/15/1/126 |
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| author | Emad Norouzi Esfahani Tomas Knedlik Sang Hun Shin Ana Paula Magalhães Rebelo Agnese De Mario Caterina Vianello Luca Persano Elena Rampazzo Paolo Edomi Camilla Bean Dario Brunetti Luca Scorrano Samuele Greco Marco Gerdol Marta Giacomello |
| author_facet | Emad Norouzi Esfahani Tomas Knedlik Sang Hun Shin Ana Paula Magalhães Rebelo Agnese De Mario Caterina Vianello Luca Persano Elena Rampazzo Paolo Edomi Camilla Bean Dario Brunetti Luca Scorrano Samuele Greco Marco Gerdol Marta Giacomello |
| author_sort | Emad Norouzi Esfahani |
| collection | DOAJ |
| description | Neural progenitor cells (NPCs) are often used to study the subcellular mechanisms underlying differentiation into neurons in vitro. Works published to date have focused on the pathways that distinguish undifferentiated NPCs from mature neurons, neglecting the earlier and intermediate stages of this process. Current evidence suggests that mitochondria interaction with the ER is fundamental to a wide range of intracellular processes. However, it is not clear whether and how the mitochondria–ER interactions differ between NPCs and their differentiated counterparts. Here we take advantage of the widely used NPC line LUHMES to provide hints on the mitochondrial dynamic trait changes that occur during the first stage of their maturation into dopaminergic-like neurons. We observed that the morphology of mitochondria, their interaction with the ER, and the expression of several mitochondria–ER contact site resident proteins change, which suggests the potential contribution of mitochondria dynamics to NPC differentiation. Further studies will be needed to explore in depth these changes, and their functional outcomes, which may be relevant to the scientific community focusing on embryonic neurogenesis and developmental neurotoxicity. |
| format | Article |
| id | doaj-art-af56c2764b89428eb0cdc484b0b86113 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-af56c2764b89428eb0cdc484b0b861132025-01-24T13:25:17ZengMDPI AGBiomolecules2218-273X2025-01-0115112610.3390/biom15010126Remodeling of Mitochondria–Endoplasmic Reticulum Contact Sites Accompanies LUHMES DifferentiationEmad Norouzi Esfahani0Tomas Knedlik1Sang Hun Shin2Ana Paula Magalhães Rebelo3Agnese De Mario4Caterina Vianello5Luca Persano6Elena Rampazzo7Paolo Edomi8Camilla Bean9Dario Brunetti10Luca Scorrano11Samuele Greco12Marco Gerdol13Marta Giacomello14Department of Biology, University of Padua, 35131 Padua, ItalyDepartment of Biology, University of Padua, 35131 Padua, ItalyDepartment of Biology, University of Padua, 35131 Padua, ItalyDepartment of Biology, University of Padua, 35131 Padua, ItalyDepartment of Biomedical Science, University of Padua, 35131 Padua, ItalyDepartment of Biology, University of Padua, 35131 Padua, ItalyDepartment of Women’s and Children’s Health, University of Padua, 35128 Padua, ItalyDepartment of Women’s and Children’s Health, University of Padua, 35128 Padua, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Medicine, University of Udine, 33100 Udine, ItalyUnit of Medical Genetics and Neurogenetics, Fondazione IRCCS Istituto Neurologico “C. Besta”, 20126 Milan, ItalyDepartment of Biology, University of Padua, 35131 Padua, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Biology, University of Padua, 35131 Padua, ItalyNeural progenitor cells (NPCs) are often used to study the subcellular mechanisms underlying differentiation into neurons in vitro. Works published to date have focused on the pathways that distinguish undifferentiated NPCs from mature neurons, neglecting the earlier and intermediate stages of this process. Current evidence suggests that mitochondria interaction with the ER is fundamental to a wide range of intracellular processes. However, it is not clear whether and how the mitochondria–ER interactions differ between NPCs and their differentiated counterparts. Here we take advantage of the widely used NPC line LUHMES to provide hints on the mitochondrial dynamic trait changes that occur during the first stage of their maturation into dopaminergic-like neurons. We observed that the morphology of mitochondria, their interaction with the ER, and the expression of several mitochondria–ER contact site resident proteins change, which suggests the potential contribution of mitochondria dynamics to NPC differentiation. Further studies will be needed to explore in depth these changes, and their functional outcomes, which may be relevant to the scientific community focusing on embryonic neurogenesis and developmental neurotoxicity.https://www.mdpi.com/2218-273X/15/1/126Neural precursor cellsLUHMESmitochondriamitochondria–ER contact sitesMERCsdifferentially expressed genes |
| spellingShingle | Emad Norouzi Esfahani Tomas Knedlik Sang Hun Shin Ana Paula Magalhães Rebelo Agnese De Mario Caterina Vianello Luca Persano Elena Rampazzo Paolo Edomi Camilla Bean Dario Brunetti Luca Scorrano Samuele Greco Marco Gerdol Marta Giacomello Remodeling of Mitochondria–Endoplasmic Reticulum Contact Sites Accompanies LUHMES Differentiation Biomolecules Neural precursor cells LUHMES mitochondria mitochondria–ER contact sites MERCs differentially expressed genes |
| title | Remodeling of Mitochondria–Endoplasmic Reticulum Contact Sites Accompanies LUHMES Differentiation |
| title_full | Remodeling of Mitochondria–Endoplasmic Reticulum Contact Sites Accompanies LUHMES Differentiation |
| title_fullStr | Remodeling of Mitochondria–Endoplasmic Reticulum Contact Sites Accompanies LUHMES Differentiation |
| title_full_unstemmed | Remodeling of Mitochondria–Endoplasmic Reticulum Contact Sites Accompanies LUHMES Differentiation |
| title_short | Remodeling of Mitochondria–Endoplasmic Reticulum Contact Sites Accompanies LUHMES Differentiation |
| title_sort | remodeling of mitochondria endoplasmic reticulum contact sites accompanies luhmes differentiation |
| topic | Neural precursor cells LUHMES mitochondria mitochondria–ER contact sites MERCs differentially expressed genes |
| url | https://www.mdpi.com/2218-273X/15/1/126 |
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