Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry
Osteosarcoma (OS) is the most common malignant bone tumor. To identify OS-related specific proteins for early diagnosis of OS, a novel approach, surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry (SELDI-TOF-MS) to serum samples from 25 OS patients, 16 osteochondroma, and 2...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/649075 |
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| author | Jianli Gu Jitian Li Manyu Huang Zhiyong Zhang Dongsheng Li Guoying Song Xingpo Ding Wuyin Li |
| author_facet | Jianli Gu Jitian Li Manyu Huang Zhiyong Zhang Dongsheng Li Guoying Song Xingpo Ding Wuyin Li |
| author_sort | Jianli Gu |
| collection | DOAJ |
| description | Osteosarcoma (OS) is the most common malignant bone tumor. To identify OS-related specific proteins for early diagnosis of OS, a novel approach, surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry (SELDI-TOF-MS) to serum samples from 25 OS patients, 16 osteochondroma, and 26 age-matched normal human volunteers as controls, was performed. Two proteins showed a significantly different expression in OS serum samples from control groups. Proteomic profiles and external leave-one-out cross-validation analysis showed that the correct rate of allocation, the sensitivity, and the specificity of diagnosis were 100%. These two proteins were further identified by searching the EPO-KB database, and one of the proteins identified as Serine rich region profile is involved in various cellular signaling cascades and tumor genesis. The presence of these two proteins in OS patients but absence from premalignant and normal human controls implied that they can be potential biomarkers for early diagnosis of OS. |
| format | Article |
| id | doaj-art-af147d84bcc1465fa945d0949899b695 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-af147d84bcc1465fa945d0949899b6952025-02-03T05:58:50ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/649075649075Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser Desorption/Ionization-Time-of-Flight Mass SpectrometryJianli Gu0Jitian Li1Manyu Huang2Zhiyong Zhang3Dongsheng Li4Guoying Song5Xingpo Ding6Wuyin Li7Luoyang Orthopedic-Traumatological Hospital, Luoyang Institute of Orthopedic and Traumatology, Henan Province, Luoyang 471002, ChinaDepartment of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USALuoyang Orthopedic-Traumatological Hospital, Luoyang Institute of Orthopedic and Traumatology, Henan Province, Luoyang 471002, ChinaLuoyang Orthopedic-Traumatological Hospital, Luoyang Institute of Orthopedic and Traumatology, Henan Province, Luoyang 471002, ChinaLuoyang Orthopedic-Traumatological Hospital, Luoyang Institute of Orthopedic and Traumatology, Henan Province, Luoyang 471002, ChinaLuoyang Orthopedic-Traumatological Hospital, Luoyang Institute of Orthopedic and Traumatology, Henan Province, Luoyang 471002, ChinaLuoyang Orthopedic-Traumatological Hospital, Luoyang Institute of Orthopedic and Traumatology, Henan Province, Luoyang 471002, ChinaLuoyang Orthopedic-Traumatological Hospital, Luoyang Institute of Orthopedic and Traumatology, Henan Province, Luoyang 471002, ChinaOsteosarcoma (OS) is the most common malignant bone tumor. To identify OS-related specific proteins for early diagnosis of OS, a novel approach, surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry (SELDI-TOF-MS) to serum samples from 25 OS patients, 16 osteochondroma, and 26 age-matched normal human volunteers as controls, was performed. Two proteins showed a significantly different expression in OS serum samples from control groups. Proteomic profiles and external leave-one-out cross-validation analysis showed that the correct rate of allocation, the sensitivity, and the specificity of diagnosis were 100%. These two proteins were further identified by searching the EPO-KB database, and one of the proteins identified as Serine rich region profile is involved in various cellular signaling cascades and tumor genesis. The presence of these two proteins in OS patients but absence from premalignant and normal human controls implied that they can be potential biomarkers for early diagnosis of OS.http://dx.doi.org/10.1155/2014/649075 |
| spellingShingle | Jianli Gu Jitian Li Manyu Huang Zhiyong Zhang Dongsheng Li Guoying Song Xingpo Ding Wuyin Li Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry Journal of Immunology Research |
| title | Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry |
| title_full | Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry |
| title_fullStr | Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry |
| title_full_unstemmed | Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry |
| title_short | Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry |
| title_sort | identification of osteosarcoma related specific proteins in serum samples using surface enhanced laser desorption ionization time of flight mass spectrometry |
| url | http://dx.doi.org/10.1155/2014/649075 |
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