Activity of photosynthetic pigments and the antioxidant system in sunflower under drought stress
Background. The study is of particular importance in view of the increasing pace of global climate aridization. The article highlights the results of an experiment on the effect of drought on the physiological status of seedlings of cv. ‘Poseidon 625’ representing an important food crop – sunflower...
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| Format: | Article |
| Language: | English |
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N.I. Vavilov All-Russian Institute of Plant Genetic Resources
2024-10-01
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| Series: | Труды по прикладной ботанике, генетике и селекции |
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| Online Access: | https://elpub.vir.nw.ru/jour/article/view/2028 |
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| author | D. G. Fedorova N. M. Nazarova B. S. Ukenov |
| author_facet | D. G. Fedorova N. M. Nazarova B. S. Ukenov |
| author_sort | D. G. Fedorova |
| collection | DOAJ |
| description | Background. The study is of particular importance in view of the increasing pace of global climate aridization. The article highlights the results of an experiment on the effect of drought on the physiological status of seedlings of cv. ‘Poseidon 625’ representing an important food crop – sunflower (Helianthus annuus L.). Materials and methods. The experiment included a group of control samples grown with sufficient moisture and four impact groups subjected to osmotic stress. The intensity of accumulation of lipid peroxidation products (LPP) was measured by the reaction of malondialdehyde (MDA) with thiobarbeturic acid; catalase activity was assessed by a photocolorimetric method based on the interaction between hydrogen peroxide and potassium iodide; the content of pigments (Chl a, Chl b, Сar) was calculated spectrophotometrically in acetone extract. Results. The degree of POL accumulation in the impact groups of the experiment was found to be many times higher than the values in the control samples, which was confirmed by a rapid increase in the MDA concentration in response to a growing water shortage. The accumulation of oxygen free radicals triggered the mechanisms of antioxidant protection of seedlings by synthesizing catalase, the concentration of which increased proportionally to the accumulation of POL. At the same time, the rapid accumulation of POL in the absence of irrigation and under osmotic stress of 3 and 5 atm led to a suppression of low-molecular-weight components of protection (carotenoids) and activation of their synthesis only when critical values of osmotic stress were reached. Conclusion. As a result of the experiment, catalase was identified as the main component of antioxidant protection in sunflower seedlings. Due to the activation of its synthesis, the concentrations of Chl a and Chl b decreased, attesting to the activation of the mechanisms protecting the photosynthetic activity in seedlings, and their antioxidant status. |
| format | Article |
| id | doaj-art-af0d007e79a04b4ba551bb035c3edd6b |
| institution | Kabale University |
| issn | 2227-8834 2619-0982 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | N.I. Vavilov All-Russian Institute of Plant Genetic Resources |
| record_format | Article |
| series | Труды по прикладной ботанике, генетике и селекции |
| spelling | doaj-art-af0d007e79a04b4ba551bb035c3edd6b2025-02-03T08:31:17ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822024-10-011853717710.30901/2227-8834-2024-3-71-77816Activity of photosynthetic pigments and the antioxidant system in sunflower under drought stressD. G. Fedorova0N. M. Nazarova1B. S. Ukenov2Orenburg State UniversityOrenburg State UniversityOrenburg State UniversityBackground. The study is of particular importance in view of the increasing pace of global climate aridization. The article highlights the results of an experiment on the effect of drought on the physiological status of seedlings of cv. ‘Poseidon 625’ representing an important food crop – sunflower (Helianthus annuus L.). Materials and methods. The experiment included a group of control samples grown with sufficient moisture and four impact groups subjected to osmotic stress. The intensity of accumulation of lipid peroxidation products (LPP) was measured by the reaction of malondialdehyde (MDA) with thiobarbeturic acid; catalase activity was assessed by a photocolorimetric method based on the interaction between hydrogen peroxide and potassium iodide; the content of pigments (Chl a, Chl b, Сar) was calculated spectrophotometrically in acetone extract. Results. The degree of POL accumulation in the impact groups of the experiment was found to be many times higher than the values in the control samples, which was confirmed by a rapid increase in the MDA concentration in response to a growing water shortage. The accumulation of oxygen free radicals triggered the mechanisms of antioxidant protection of seedlings by synthesizing catalase, the concentration of which increased proportionally to the accumulation of POL. At the same time, the rapid accumulation of POL in the absence of irrigation and under osmotic stress of 3 and 5 atm led to a suppression of low-molecular-weight components of protection (carotenoids) and activation of their synthesis only when critical values of osmotic stress were reached. Conclusion. As a result of the experiment, catalase was identified as the main component of antioxidant protection in sunflower seedlings. Due to the activation of its synthesis, the concentrations of Chl a and Chl b decreased, attesting to the activation of the mechanisms protecting the photosynthetic activity in seedlings, and their antioxidant status.https://elpub.vir.nw.ru/jour/article/view/2028<i>helianthus annuus</i> l.seedlingschlorophyllcarotenoidscatalasemalondialdehyde |
| spellingShingle | D. G. Fedorova N. M. Nazarova B. S. Ukenov Activity of photosynthetic pigments and the antioxidant system in sunflower under drought stress Труды по прикладной ботанике, генетике и селекции <i>helianthus annuus</i> l. seedlings chlorophyll carotenoids catalase malondialdehyde |
| title | Activity of photosynthetic pigments and the antioxidant system in sunflower under drought stress |
| title_full | Activity of photosynthetic pigments and the antioxidant system in sunflower under drought stress |
| title_fullStr | Activity of photosynthetic pigments and the antioxidant system in sunflower under drought stress |
| title_full_unstemmed | Activity of photosynthetic pigments and the antioxidant system in sunflower under drought stress |
| title_short | Activity of photosynthetic pigments and the antioxidant system in sunflower under drought stress |
| title_sort | activity of photosynthetic pigments and the antioxidant system in sunflower under drought stress |
| topic | <i>helianthus annuus</i> l. seedlings chlorophyll carotenoids catalase malondialdehyde |
| url | https://elpub.vir.nw.ru/jour/article/view/2028 |
| work_keys_str_mv | AT dgfedorova activityofphotosyntheticpigmentsandtheantioxidantsysteminsunflowerunderdroughtstress AT nmnazarova activityofphotosyntheticpigmentsandtheantioxidantsysteminsunflowerunderdroughtstress AT bsukenov activityofphotosyntheticpigmentsandtheantioxidantsysteminsunflowerunderdroughtstress |