Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia
Danqi pill (DQP) is a widely prescribed traditional Chinese medicine (TCM) in the treatment of cardiovascular diseases. The objective of this study is to systematically characterize altered gene expression pattern induced by myocardial ischemia (MI) in a rat model and to investigate the effects of D...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2018/9469670 |
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| author | Qiyan Wang Hui Meng Qian Zhang Tianjiao Shi Xuefeng Zhang Mingyan Shao Linghui Lu Jing Wang Wei Wang Chun Li Yong Wang |
| author_facet | Qiyan Wang Hui Meng Qian Zhang Tianjiao Shi Xuefeng Zhang Mingyan Shao Linghui Lu Jing Wang Wei Wang Chun Li Yong Wang |
| author_sort | Qiyan Wang |
| collection | DOAJ |
| description | Danqi pill (DQP) is a widely prescribed traditional Chinese medicine (TCM) in the treatment of cardiovascular diseases. The objective of this study is to systematically characterize altered gene expression pattern induced by myocardial ischemia (MI) in a rat model and to investigate the effects of DQP on global gene expression. Global mRNA expression was measured. Differentially expressed genes among the sham group, model group, and DQP group were analyzed. The gene ontology enrichment analysis and pathway analysis of differentially expressed genes were carried out. We quantified 10,813 genes. Compared with the sham group, expressions of 339 genes were upregulated and 177 genes were downregulated in the model group. The upregulated genes were enriched in extracellular matrix organization, response to wounding, and defense response pathways. Downregulated genes were enriched in fatty acid metabolism, pyruvate metabolism, PPAR signaling pathways, and so forth. This indicated that energy metabolic disorders occurred in rats with MI. In the DQP group, expressions of genes in the altered pathways were regulated back towards normal levels. DQP reversed expression of 313 of the 516 differentially expressed genes in the model group. This study provides insight into the multitarget mechanism of TCM in the treatment of complex diseases. |
| format | Article |
| id | doaj-art-af00d225e8de41c487fb56dfe85a5059 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-af00d225e8de41c487fb56dfe85a50592025-02-03T01:22:12ZengWileyInternational Journal of Genomics2314-436X2314-43782018-01-01201810.1155/2018/94696709469670Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial IschemiaQiyan Wang0Hui Meng1Qian Zhang2Tianjiao Shi3Xuefeng Zhang4Mingyan Shao5Linghui Lu6Jing Wang7Wei Wang8Chun Li9Yong Wang10School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaModern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaStaidson (Beijing) Biopharmaceuticals Co., Ltd., Beijing 100176, ChinaSchool of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaModern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaDanqi pill (DQP) is a widely prescribed traditional Chinese medicine (TCM) in the treatment of cardiovascular diseases. The objective of this study is to systematically characterize altered gene expression pattern induced by myocardial ischemia (MI) in a rat model and to investigate the effects of DQP on global gene expression. Global mRNA expression was measured. Differentially expressed genes among the sham group, model group, and DQP group were analyzed. The gene ontology enrichment analysis and pathway analysis of differentially expressed genes were carried out. We quantified 10,813 genes. Compared with the sham group, expressions of 339 genes were upregulated and 177 genes were downregulated in the model group. The upregulated genes were enriched in extracellular matrix organization, response to wounding, and defense response pathways. Downregulated genes were enriched in fatty acid metabolism, pyruvate metabolism, PPAR signaling pathways, and so forth. This indicated that energy metabolic disorders occurred in rats with MI. In the DQP group, expressions of genes in the altered pathways were regulated back towards normal levels. DQP reversed expression of 313 of the 516 differentially expressed genes in the model group. This study provides insight into the multitarget mechanism of TCM in the treatment of complex diseases.http://dx.doi.org/10.1155/2018/9469670 |
| spellingShingle | Qiyan Wang Hui Meng Qian Zhang Tianjiao Shi Xuefeng Zhang Mingyan Shao Linghui Lu Jing Wang Wei Wang Chun Li Yong Wang Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia International Journal of Genomics |
| title | Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia |
| title_full | Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia |
| title_fullStr | Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia |
| title_full_unstemmed | Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia |
| title_short | Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia |
| title_sort | multitarget effects of danqi pill on global gene expression changes in myocardial ischemia |
| url | http://dx.doi.org/10.1155/2018/9469670 |
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