The nonglycosylated variant in therapeutic monoclonal antibodies preferentially forms large aggregates under typical thermal stresses used in forced degradation studies

The nonglycosylated variant in therapeutic monoclonal antibodies preferentially forms large aggregates under typical thermal stresses used in forced degradation studies

Monoclonal antibodies (mAbs) feature a conserved N-linked glycosylation site in the CH2 domain, which exhibits heterogeneities in both occupancy and glycan structures. Previous studies have suggested that the unoccupied (nonglycosylated) variant exhibits decreased thermal stability, potentially impa...

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Main Authors: Gao-Yuan Liu, Jenny Kim Kim, Shuli Tang, Yuetian Yan, Mandi Hopkins, Dalia Laredo, Teng-Chieh Yang, James Mutino, Douglas E. Kamen, Kenneth S. Graham, Mohammed Shameem, Shunhai Wang, Ning Li
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:mAbs
Subjects:
monoclonal antibodies
nonglycosylated mAb
thermal stability
Aggregates
SEC-PCD-MS
Online Access:https://www.tandfonline.com/doi/10.1080/19420862.2025.2543768
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https://www.tandfonline.com/doi/10.1080/19420862.2025.2543768

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