Microspectrometer-Enabled Real-Time Concentration Monitoring in the Microfluidic Protein Enrichment Chip
This study presents a novel microspectrometer-integrated microfluidic system for real-time protein concentration monitoring. The device employs electrokinetic principles for efficient protein preconcentration in a PDMS and Nafion film channel. Using FITC-labeled BSA as a model protein, the system de...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/1/1 |
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| author | Dong-Li Li Wen-Shu Huang Yi Hung Wu Chun-Ping Jen |
| author_facet | Dong-Li Li Wen-Shu Huang Yi Hung Wu Chun-Ping Jen |
| author_sort | Dong-Li Li |
| collection | DOAJ |
| description | This study presents a novel microspectrometer-integrated microfluidic system for real-time protein concentration monitoring. The device employs electrokinetic principles for efficient protein preconcentration in a PDMS and Nafion film channel. Using FITC-labeled BSA as a model protein, the system demonstrated a linear correlation between protein concentration and absorbance at 491 nm. Notably, it achieved a 833-fold concentration increase from an initial 10 nM within 20 min. The compact microspectrometer system offers enhanced accuracy and sensitivity compared to traditional fluorescence microscopy methods. This innovation presents a promising solution for portable and point-of-care diagnostic applications, facilitating timely disease detection and monitoring. The findings highlight the potential for this technology to advance protein analysis and biomarker discovery in clinical settings, potentially improving patient outcomes through enhanced diagnostic capabilities. |
| format | Article |
| id | doaj-art-aed342b8f7b84a489d72a2af3b7c72d4 |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-aed342b8f7b84a489d72a2af3b7c72d42025-01-24T13:25:23ZengMDPI AGBiosensors2079-63742024-12-01151110.3390/bios15010001Microspectrometer-Enabled Real-Time Concentration Monitoring in the Microfluidic Protein Enrichment ChipDong-Li Li0Wen-Shu Huang1Yi Hung Wu2Chun-Ping Jen3Fisheries College, Jimei University, Xiamen 361021, ChinaFisheries College, Jimei University, Xiamen 361021, ChinaDepartment of Mechanical Engineering and Advanced Institute of Manufacturing for High-Tech Innovations, National Chung Cheng University, Chia-Yi 62102, TaiwanDepartment of Mechanical Engineering and Advanced Institute of Manufacturing for High-Tech Innovations, National Chung Cheng University, Chia-Yi 62102, TaiwanThis study presents a novel microspectrometer-integrated microfluidic system for real-time protein concentration monitoring. The device employs electrokinetic principles for efficient protein preconcentration in a PDMS and Nafion film channel. Using FITC-labeled BSA as a model protein, the system demonstrated a linear correlation between protein concentration and absorbance at 491 nm. Notably, it achieved a 833-fold concentration increase from an initial 10 nM within 20 min. The compact microspectrometer system offers enhanced accuracy and sensitivity compared to traditional fluorescence microscopy methods. This innovation presents a promising solution for portable and point-of-care diagnostic applications, facilitating timely disease detection and monitoring. The findings highlight the potential for this technology to advance protein analysis and biomarker discovery in clinical settings, potentially improving patient outcomes through enhanced diagnostic capabilities.https://www.mdpi.com/2079-6374/15/1/1microfluidic chipsprotein detectionmicrospectrometerfluorescencepreconcentration |
| spellingShingle | Dong-Li Li Wen-Shu Huang Yi Hung Wu Chun-Ping Jen Microspectrometer-Enabled Real-Time Concentration Monitoring in the Microfluidic Protein Enrichment Chip Biosensors microfluidic chips protein detection microspectrometer fluorescence preconcentration |
| title | Microspectrometer-Enabled Real-Time Concentration Monitoring in the Microfluidic Protein Enrichment Chip |
| title_full | Microspectrometer-Enabled Real-Time Concentration Monitoring in the Microfluidic Protein Enrichment Chip |
| title_fullStr | Microspectrometer-Enabled Real-Time Concentration Monitoring in the Microfluidic Protein Enrichment Chip |
| title_full_unstemmed | Microspectrometer-Enabled Real-Time Concentration Monitoring in the Microfluidic Protein Enrichment Chip |
| title_short | Microspectrometer-Enabled Real-Time Concentration Monitoring in the Microfluidic Protein Enrichment Chip |
| title_sort | microspectrometer enabled real time concentration monitoring in the microfluidic protein enrichment chip |
| topic | microfluidic chips protein detection microspectrometer fluorescence preconcentration |
| url | https://www.mdpi.com/2079-6374/15/1/1 |
| work_keys_str_mv | AT donglili microspectrometerenabledrealtimeconcentrationmonitoringinthemicrofluidicproteinenrichmentchip AT wenshuhuang microspectrometerenabledrealtimeconcentrationmonitoringinthemicrofluidicproteinenrichmentchip AT yihungwu microspectrometerenabledrealtimeconcentrationmonitoringinthemicrofluidicproteinenrichmentchip AT chunpingjen microspectrometerenabledrealtimeconcentrationmonitoringinthemicrofluidicproteinenrichmentchip |