IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway
Mesenchymal stem cells (MSCs) are known for homing to sites of injury in response to signals of cellular damage. However, the mechanisms of how cytokines recruit stem cells to target tissue are still unclear. In this study, we found that the proinflammation cytokine interleukin-1β (IL-1β) promotes m...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/3524759 |
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| _version_ | 1832549604756815872 |
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| author | Ming-Siou Chen Cheng-Yu Lin Yun-Hsuan Chiu Chie-Pein Chen Pei-Jiun Tsai Hwai-Shi Wang |
| author_facet | Ming-Siou Chen Cheng-Yu Lin Yun-Hsuan Chiu Chie-Pein Chen Pei-Jiun Tsai Hwai-Shi Wang |
| author_sort | Ming-Siou Chen |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are known for homing to sites of injury in response to signals of cellular damage. However, the mechanisms of how cytokines recruit stem cells to target tissue are still unclear. In this study, we found that the proinflammation cytokine interleukin-1β (IL-1β) promotes mesenchymal stem cell migration. The cDNA microarray data show that IL-1β induces matrix metalloproteinase-1 (MMP-1) expression. We then used quantitative real-time PCR and MMP-1 ELISA to verify the results. MMP-1 siRNA transfected MSCs, and MSC pretreatment with IL-1β inhibitor interleukin-1 receptor antagonist (IL-1RA), MMP tissue inhibitor of metalloproteinase 1 (TIMP1), tissue inhibitor of metalloproteinase 2 (TIMP2), MMP-1 inhibitor GM6001, and protease-activated receptor 1 (PAR1) inhibitor SCH79797 confirms that PAR1 protein signaling pathway leads to IL-1β-induced cell migration. In conclusion, IL-1β promotes the secretion of MMP-1, which then activates the PAR1 and G-protein-coupled signal pathways to promote mesenchymal stem cell migration. |
| format | Article |
| id | doaj-art-aecf38fa42554e999044dd898307cf44 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-aecf38fa42554e999044dd898307cf442025-02-03T06:10:52ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/35247593524759IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling PathwayMing-Siou Chen0Cheng-Yu Lin1Yun-Hsuan Chiu2Chie-Pein Chen3Pei-Jiun Tsai4Hwai-Shi Wang5Institute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei, TaiwanInstitute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei, TaiwanInstitute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei, TaiwanDivision of High Risk Pregnancy, Mackay Memorial Hospital, Taipei, TaiwanInstitute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei, TaiwanInstitute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei, TaiwanMesenchymal stem cells (MSCs) are known for homing to sites of injury in response to signals of cellular damage. However, the mechanisms of how cytokines recruit stem cells to target tissue are still unclear. In this study, we found that the proinflammation cytokine interleukin-1β (IL-1β) promotes mesenchymal stem cell migration. The cDNA microarray data show that IL-1β induces matrix metalloproteinase-1 (MMP-1) expression. We then used quantitative real-time PCR and MMP-1 ELISA to verify the results. MMP-1 siRNA transfected MSCs, and MSC pretreatment with IL-1β inhibitor interleukin-1 receptor antagonist (IL-1RA), MMP tissue inhibitor of metalloproteinase 1 (TIMP1), tissue inhibitor of metalloproteinase 2 (TIMP2), MMP-1 inhibitor GM6001, and protease-activated receptor 1 (PAR1) inhibitor SCH79797 confirms that PAR1 protein signaling pathway leads to IL-1β-induced cell migration. In conclusion, IL-1β promotes the secretion of MMP-1, which then activates the PAR1 and G-protein-coupled signal pathways to promote mesenchymal stem cell migration.http://dx.doi.org/10.1155/2018/3524759 |
| spellingShingle | Ming-Siou Chen Cheng-Yu Lin Yun-Hsuan Chiu Chie-Pein Chen Pei-Jiun Tsai Hwai-Shi Wang IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway Stem Cells International |
| title | IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway |
| title_full | IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway |
| title_fullStr | IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway |
| title_full_unstemmed | IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway |
| title_short | IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway |
| title_sort | il 1β induced matrix metalloprotease 1 promotes mesenchymal stem cell migration via par1 and g protein coupled signaling pathway |
| url | http://dx.doi.org/10.1155/2018/3524759 |
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