A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSE
National Oceanic and Atmospheric Administration (NOAA) Method NMFS-NWFSC-59 2004 is currently used to quantitatively analyze seafood for polycyclic aromatic hydrocarbon (PAH) contamination, especially following events such as the Deepwater Horizon oil rig explosion that released millions of barrels...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/359629 |
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| author | Edward A. Pfannkoch John R. Stuff Jacqueline A. Whitecavage John M. Blevins Kathryn A. Seely Jeffery H. Moran |
| author_facet | Edward A. Pfannkoch John R. Stuff Jacqueline A. Whitecavage John M. Blevins Kathryn A. Seely Jeffery H. Moran |
| author_sort | Edward A. Pfannkoch |
| collection | DOAJ |
| description | National Oceanic and Atmospheric Administration (NOAA) Method NMFS-NWFSC-59 2004 is currently used to quantitatively analyze seafood for polycyclic aromatic hydrocarbon (PAH) contamination, especially following events such as the Deepwater Horizon oil rig explosion that released millions of barrels of crude oil into the Gulf of Mexico. This method has limited throughput capacity; hence, alternative methods are necessary to meet analytical demands after such events. Stir bar sorptive extraction (SBSE) is an effective technique to extract trace PAHs in water and the quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction strategy effectively extracts PAHs from complex food matrices. This study uses SBSE to concentrate PAHs and eliminate matrix interference from QuEChERS extracts of seafood, specifically oysters, fish, and shrimp. This method provides acceptable recovery (65–138%) linear calibrations and is sensitive (LOD = 0.02 ppb, LOQ = 0.06 ppb) while providing higher throughput and maintaining equivalency between NOAA 2004 as determined by analysis of NIST SRM 1974b mussel tissue. |
| format | Article |
| id | doaj-art-aecf0953ed9143e987f0a39cee231c06 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-aecf0953ed9143e987f0a39cee231c062025-02-03T05:51:35ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792015-01-01201510.1155/2015/359629359629A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSEEdward A. Pfannkoch0John R. Stuff1Jacqueline A. Whitecavage2John M. Blevins3Kathryn A. Seely4Jeffery H. Moran5GERSTEL Inc., 701 Digital Drive, Suite J, Linthicum, MD 21090, USAGERSTEL Inc., 701 Digital Drive, Suite J, Linthicum, MD 21090, USAGERSTEL Inc., 701 Digital Drive, Suite J, Linthicum, MD 21090, USAArkansas Department of Health, Public Health Laboratory, 201 S. Monroe Street, Little Rock, AR 72205, USAArkansas Department of Health, Public Health Laboratory, 201 S. Monroe Street, Little Rock, AR 72205, USAArkansas Department of Health, Public Health Laboratory, 201 S. Monroe Street, Little Rock, AR 72205, USANational Oceanic and Atmospheric Administration (NOAA) Method NMFS-NWFSC-59 2004 is currently used to quantitatively analyze seafood for polycyclic aromatic hydrocarbon (PAH) contamination, especially following events such as the Deepwater Horizon oil rig explosion that released millions of barrels of crude oil into the Gulf of Mexico. This method has limited throughput capacity; hence, alternative methods are necessary to meet analytical demands after such events. Stir bar sorptive extraction (SBSE) is an effective technique to extract trace PAHs in water and the quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction strategy effectively extracts PAHs from complex food matrices. This study uses SBSE to concentrate PAHs and eliminate matrix interference from QuEChERS extracts of seafood, specifically oysters, fish, and shrimp. This method provides acceptable recovery (65–138%) linear calibrations and is sensitive (LOD = 0.02 ppb, LOQ = 0.06 ppb) while providing higher throughput and maintaining equivalency between NOAA 2004 as determined by analysis of NIST SRM 1974b mussel tissue.http://dx.doi.org/10.1155/2015/359629 |
| spellingShingle | Edward A. Pfannkoch John R. Stuff Jacqueline A. Whitecavage John M. Blevins Kathryn A. Seely Jeffery H. Moran A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSE International Journal of Analytical Chemistry |
| title | A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSE |
| title_full | A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSE |
| title_fullStr | A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSE |
| title_full_unstemmed | A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSE |
| title_short | A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSE |
| title_sort | high throughput method for measuring polycyclic aromatic hydrocarbons in seafood using quechers extraction and sbse |
| url | http://dx.doi.org/10.1155/2015/359629 |
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