Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes
Acinetobacter baumannii is one of the most important nosocomial opportunistic pathogen worldwide. In addition, obesity has been associated with an increased risk of nosocomial infection, suggesting that there may be an association between A. baumannii and white adipose tissue. However, the effects o...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/9039302 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561107869368320 |
|---|---|
| author | Yuka Unno Yoshinori Sato Satoshi Nishida Akiyo Nakano Ryuichi Nakano Tsuneyuki Ubagai Yasuo Ono |
| author_facet | Yuka Unno Yoshinori Sato Satoshi Nishida Akiyo Nakano Ryuichi Nakano Tsuneyuki Ubagai Yasuo Ono |
| author_sort | Yuka Unno |
| collection | DOAJ |
| description | Acinetobacter baumannii is one of the most important nosocomial opportunistic pathogen worldwide. In addition, obesity has been associated with an increased risk of nosocomial infection, suggesting that there may be an association between A. baumannii and white adipose tissue. However, the effects of A. baumannii on adipocytes have not been well studied at the molecular level. Here, we investigated the potential role of A. baumannii-derived lipopolysaccharides (LPS) as signaling molecules that affect adipocyte functionality. We tested the effect of increasing concentrations of A. baumannii-derived LPS (10, 100, or 1000 ng/mL) on the 3T3-L1 adipocyte cell line. Exposure to LPS was found to increase the expression of several adipokines (e.g., MIP-2, MCP-1, TNF-α, IL-6, lipocalin-2, and FABP4) in 3T3-L1 adipocytes and significantly reduced the expression of leptin and adiponectin. The effects of A. baumannii-derived LPS on MIP-2 expression were similar in comparison with that of LPS prepared from Pseudomonas aeruginosa and Escherichia coli in our cell culture-based system. This study suggests that A. baumannii-derived LPS functions as a signaling molecule that impacts the inflammatory function of white adipose tissue on the level of gene expression. |
| format | Article |
| id | doaj-art-ae7aa95dbbf04ca8a6f435cc0fdba01b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ae7aa95dbbf04ca8a6f435cc0fdba01b2025-02-03T01:25:55ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/90393029039302Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 AdipocytesYuka Unno0Yoshinori Sato1Satoshi Nishida2Akiyo Nakano3Ryuichi Nakano4Tsuneyuki Ubagai5Yasuo Ono6Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi, Tokyo 173-8605, JapanDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi, Tokyo 173-8605, JapanDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi, Tokyo 173-8605, JapanDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi, Tokyo 173-8605, JapanDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi, Tokyo 173-8605, JapanDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi, Tokyo 173-8605, JapanDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi, Tokyo 173-8605, JapanAcinetobacter baumannii is one of the most important nosocomial opportunistic pathogen worldwide. In addition, obesity has been associated with an increased risk of nosocomial infection, suggesting that there may be an association between A. baumannii and white adipose tissue. However, the effects of A. baumannii on adipocytes have not been well studied at the molecular level. Here, we investigated the potential role of A. baumannii-derived lipopolysaccharides (LPS) as signaling molecules that affect adipocyte functionality. We tested the effect of increasing concentrations of A. baumannii-derived LPS (10, 100, or 1000 ng/mL) on the 3T3-L1 adipocyte cell line. Exposure to LPS was found to increase the expression of several adipokines (e.g., MIP-2, MCP-1, TNF-α, IL-6, lipocalin-2, and FABP4) in 3T3-L1 adipocytes and significantly reduced the expression of leptin and adiponectin. The effects of A. baumannii-derived LPS on MIP-2 expression were similar in comparison with that of LPS prepared from Pseudomonas aeruginosa and Escherichia coli in our cell culture-based system. This study suggests that A. baumannii-derived LPS functions as a signaling molecule that impacts the inflammatory function of white adipose tissue on the level of gene expression.http://dx.doi.org/10.1155/2017/9039302 |
| spellingShingle | Yuka Unno Yoshinori Sato Satoshi Nishida Akiyo Nakano Ryuichi Nakano Tsuneyuki Ubagai Yasuo Ono Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes Mediators of Inflammation |
| title | Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes |
| title_full | Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes |
| title_fullStr | Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes |
| title_full_unstemmed | Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes |
| title_short | Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes |
| title_sort | acinetobacter baumannii lipopolysaccharide influences adipokine expression in 3t3 l1 adipocytes |
| url | http://dx.doi.org/10.1155/2017/9039302 |
| work_keys_str_mv | AT yukaunno acinetobacterbaumanniilipopolysaccharideinfluencesadipokineexpressionin3t3l1adipocytes AT yoshinorisato acinetobacterbaumanniilipopolysaccharideinfluencesadipokineexpressionin3t3l1adipocytes AT satoshinishida acinetobacterbaumanniilipopolysaccharideinfluencesadipokineexpressionin3t3l1adipocytes AT akiyonakano acinetobacterbaumanniilipopolysaccharideinfluencesadipokineexpressionin3t3l1adipocytes AT ryuichinakano acinetobacterbaumanniilipopolysaccharideinfluencesadipokineexpressionin3t3l1adipocytes AT tsuneyukiubagai acinetobacterbaumanniilipopolysaccharideinfluencesadipokineexpressionin3t3l1adipocytes AT yasuoono acinetobacterbaumanniilipopolysaccharideinfluencesadipokineexpressionin3t3l1adipocytes |