Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study
Abstract Background Due to non-vascularized and aneural structure, articular cartilage has limited self-repairing capacity. The aim of this study was to investigate the revitalization of inflammatory injured articular cartilage matrices by human nasal chondrocytes (hNC). Materials and methods Cartil...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | Knee Surgery & Related Research |
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| Online Access: | https://doi.org/10.1186/s43019-024-00249-y |
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| author | Victoria A. Shestakova Ilya D. Klabukov Ilya V. Kolobaev Longfeng Rao Dmitry A. Atiakshin Michael A. Ignatyuk Mikhail E. Krasheninnikov Bagavdin G. Ahmedov Sergey A. Ivanov Peter V. Shegay Andrey D. Kaprin Denis S. Baranovskii |
| author_facet | Victoria A. Shestakova Ilya D. Klabukov Ilya V. Kolobaev Longfeng Rao Dmitry A. Atiakshin Michael A. Ignatyuk Mikhail E. Krasheninnikov Bagavdin G. Ahmedov Sergey A. Ivanov Peter V. Shegay Andrey D. Kaprin Denis S. Baranovskii |
| author_sort | Victoria A. Shestakova |
| collection | DOAJ |
| description | Abstract Background Due to non-vascularized and aneural structure, articular cartilage has limited self-repairing capacity. The aim of this study was to investigate the revitalization of inflammatory injured articular cartilage matrices by human nasal chondrocytes (hNC). Materials and methods Cartilage matrix was prepared by devitalization of articular cartilage samples obtained intraoperatively from an adult patient undergoing knee joint replacement. hNC were obtained from native tissues by enzymatic digestion with further expansion over two passages. The obtained nasal chondrocytes were used to seed decellularized scaffolds, which were then cultured in vitro for 7, 14, or 21 days in chondrogenic medium. Migration was observed by histologic staining with fast green, safranin-O, and hematoxylin and scanning electron microscopy. Biochemical analysis was performed to determine the glycosaminoglycan (GAG) and DNA content of the cartilage using dimethylmethylene blue and CyQuant Cell Proliferation Assay Kit. Results We seeded healthy and inflamed cartilage with nasal chondrocytes and found that the cells actively invade mainly pathologically altered cartilage. The results of biochemical quantitative analysis showed that the amount of DNA significantly increased by day 7 and decreased by day 14, while the quantitative values of GAGs had the opposite trend. Histological staining showed that cartilage formation occurred on day 7, intercellular spaces were filled with de novo synthesized cartilage matrix with significantly low GAG content on day 14, and newly formed GAG-rich cartilage was observed on day 21. The obtained data on cartilage regeneration were confirmed by scanning electron microscopy. Conclusions Our preliminary results showed that human nasal chondrocytes are capable of infiltrating the pathologically altered extracellular matrix of articular cartilage damaged by arthritis, thereby promoting its repair to a physiologically relevant state. Graphical Abstract |
| format | Article |
| id | doaj-art-ae5620ecc5654568b02bb9aa2b10f1db |
| institution | Kabale University |
| issn | 2234-2451 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Knee Surgery & Related Research |
| spelling | doaj-art-ae5620ecc5654568b02bb9aa2b10f1db2025-01-26T12:39:23ZengBMCKnee Surgery & Related Research2234-24512024-12-0136111010.1186/s43019-024-00249-yPathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot studyVictoria A. Shestakova0Ilya D. Klabukov1Ilya V. Kolobaev2Longfeng Rao3Dmitry A. Atiakshin4Michael A. Ignatyuk5Mikhail E. Krasheninnikov6Bagavdin G. Ahmedov7Sergey A. Ivanov8Peter V. Shegay9Andrey D. Kaprin10Denis S. Baranovskii11National Medical Research Radiological Center of the Ministry of Health of the Russian FederationNational Medical Research Radiological Center of the Ministry of Health of the Russian FederationNational Medical Research Radiological Center of the Ministry of Health of the Russian FederationETH ZurichPatrice Lumumba Peoples Friendship University of Russia (RUDN University)Patrice Lumumba Peoples Friendship University of Russia (RUDN University)Patrice Lumumba Peoples Friendship University of Russia (RUDN University)National Medical Research Center for Surgery named after A.V. Vishnevsky of the Ministry of Health of the Russian FederationNational Medical Research Radiological Center of the Ministry of Health of the Russian FederationNational Medical Research Radiological Center of the Ministry of Health of the Russian FederationNational Medical Research Radiological Center of the Ministry of Health of the Russian FederationNational Medical Research Radiological Center of the Ministry of Health of the Russian FederationAbstract Background Due to non-vascularized and aneural structure, articular cartilage has limited self-repairing capacity. The aim of this study was to investigate the revitalization of inflammatory injured articular cartilage matrices by human nasal chondrocytes (hNC). Materials and methods Cartilage matrix was prepared by devitalization of articular cartilage samples obtained intraoperatively from an adult patient undergoing knee joint replacement. hNC were obtained from native tissues by enzymatic digestion with further expansion over two passages. The obtained nasal chondrocytes were used to seed decellularized scaffolds, which were then cultured in vitro for 7, 14, or 21 days in chondrogenic medium. Migration was observed by histologic staining with fast green, safranin-O, and hematoxylin and scanning electron microscopy. Biochemical analysis was performed to determine the glycosaminoglycan (GAG) and DNA content of the cartilage using dimethylmethylene blue and CyQuant Cell Proliferation Assay Kit. Results We seeded healthy and inflamed cartilage with nasal chondrocytes and found that the cells actively invade mainly pathologically altered cartilage. The results of biochemical quantitative analysis showed that the amount of DNA significantly increased by day 7 and decreased by day 14, while the quantitative values of GAGs had the opposite trend. Histological staining showed that cartilage formation occurred on day 7, intercellular spaces were filled with de novo synthesized cartilage matrix with significantly low GAG content on day 14, and newly formed GAG-rich cartilage was observed on day 21. The obtained data on cartilage regeneration were confirmed by scanning electron microscopy. Conclusions Our preliminary results showed that human nasal chondrocytes are capable of infiltrating the pathologically altered extracellular matrix of articular cartilage damaged by arthritis, thereby promoting its repair to a physiologically relevant state. Graphical Abstracthttps://doi.org/10.1186/s43019-024-00249-yCartilageCell therapyChondrocytesPathologically altered cartilageRegenerative medicine |
| spellingShingle | Victoria A. Shestakova Ilya D. Klabukov Ilya V. Kolobaev Longfeng Rao Dmitry A. Atiakshin Michael A. Ignatyuk Mikhail E. Krasheninnikov Bagavdin G. Ahmedov Sergey A. Ivanov Peter V. Shegay Andrey D. Kaprin Denis S. Baranovskii Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study Knee Surgery & Related Research Cartilage Cell therapy Chondrocytes Pathologically altered cartilage Regenerative medicine |
| title | Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study |
| title_full | Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study |
| title_fullStr | Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study |
| title_full_unstemmed | Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study |
| title_short | Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study |
| title_sort | pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix in vitro pilot study |
| topic | Cartilage Cell therapy Chondrocytes Pathologically altered cartilage Regenerative medicine |
| url | https://doi.org/10.1186/s43019-024-00249-y |
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