Determination of Ivermectin in Medicated Feeds by Liquid Chromatography with Fluorescence Detection
A labour- and time-effective analytical procedure for determination of ivermectin in medicated feed at recommended level of 2.0 mg kg−1 has been developed and validated. The analyte was extracted from grinded feed samples with acetonitrile and derivatisated with N-methylimidazole and trifluoracetic...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/362453 |
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| author | Konrad Pietruk Piotr Jedziniak |
| author_facet | Konrad Pietruk Piotr Jedziniak |
| author_sort | Konrad Pietruk |
| collection | DOAJ |
| description | A labour- and time-effective analytical procedure for determination of ivermectin in medicated feed at recommended level of 2.0 mg kg−1 has been developed and validated. The analyte was extracted from grinded feed samples with acetonitrile and derivatisated with N-methylimidazole and trifluoracetic anhydride. The fluorescent derivatives were analysed by liquid chromatography method using C8 column. The isocratic conditions using acetonitrile, methanol, water, and tetrahydrofuran were applied. Fluorescence detection was performed at 365 nm (excitation) and 475 nm (emission) wavelengths. The total analysis time was 10 min. The validation results of the method (within-laboratory reproducibility 4.0% CV, mean recovery 100.1%) confirm the appropriate precision and accuracy of the developed method. |
| format | Article |
| id | doaj-art-ae3beee180484d59995d76f4a7a989d9 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ae3beee180484d59995d76f4a7a989d92025-02-03T06:44:38ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/362453362453Determination of Ivermectin in Medicated Feeds by Liquid Chromatography with Fluorescence DetectionKonrad Pietruk0Piotr Jedziniak1Department of Pharmacology and Toxicology, National Veterinary Research Institute, 57 Partyzantow Avenue, 24-100 Pulawy, PolandDepartment of Pharmacology and Toxicology, National Veterinary Research Institute, 57 Partyzantow Avenue, 24-100 Pulawy, PolandA labour- and time-effective analytical procedure for determination of ivermectin in medicated feed at recommended level of 2.0 mg kg−1 has been developed and validated. The analyte was extracted from grinded feed samples with acetonitrile and derivatisated with N-methylimidazole and trifluoracetic anhydride. The fluorescent derivatives were analysed by liquid chromatography method using C8 column. The isocratic conditions using acetonitrile, methanol, water, and tetrahydrofuran were applied. Fluorescence detection was performed at 365 nm (excitation) and 475 nm (emission) wavelengths. The total analysis time was 10 min. The validation results of the method (within-laboratory reproducibility 4.0% CV, mean recovery 100.1%) confirm the appropriate precision and accuracy of the developed method.http://dx.doi.org/10.1155/2013/362453 |
| spellingShingle | Konrad Pietruk Piotr Jedziniak Determination of Ivermectin in Medicated Feeds by Liquid Chromatography with Fluorescence Detection The Scientific World Journal |
| title | Determination of Ivermectin in Medicated Feeds by Liquid Chromatography with Fluorescence Detection |
| title_full | Determination of Ivermectin in Medicated Feeds by Liquid Chromatography with Fluorescence Detection |
| title_fullStr | Determination of Ivermectin in Medicated Feeds by Liquid Chromatography with Fluorescence Detection |
| title_full_unstemmed | Determination of Ivermectin in Medicated Feeds by Liquid Chromatography with Fluorescence Detection |
| title_short | Determination of Ivermectin in Medicated Feeds by Liquid Chromatography with Fluorescence Detection |
| title_sort | determination of ivermectin in medicated feeds by liquid chromatography with fluorescence detection |
| url | http://dx.doi.org/10.1155/2013/362453 |
| work_keys_str_mv | AT konradpietruk determinationofivermectininmedicatedfeedsbyliquidchromatographywithfluorescencedetection AT piotrjedziniak determinationofivermectininmedicatedfeedsbyliquidchromatographywithfluorescencedetection |