Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro
The biomaterial scaffold plays a key role in most tissue engineering strategies. Its surface properties, micropatterning, degradation, and mechanical features affect not only the generation of the tissue construct in vitro, but also its in vivo functionality. The area of myocardial tissue engineerin...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/7176154 |
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| author | Caterina Cristallini Elisa Cibrario Rocchietti Mariacristina Gagliardi Leonardo Mortati Silvia Saviozzi Elena Bellotti Valentina Turinetto Maria Paola Sassi Niccoletta Barbani Claudia Giachino |
| author_facet | Caterina Cristallini Elisa Cibrario Rocchietti Mariacristina Gagliardi Leonardo Mortati Silvia Saviozzi Elena Bellotti Valentina Turinetto Maria Paola Sassi Niccoletta Barbani Claudia Giachino |
| author_sort | Caterina Cristallini |
| collection | DOAJ |
| description | The biomaterial scaffold plays a key role in most tissue engineering strategies. Its surface properties, micropatterning, degradation, and mechanical features affect not only the generation of the tissue construct in vitro, but also its in vivo functionality. The area of myocardial tissue engineering still faces significant difficulties and challenges in the design of bioactive scaffolds, which allow composition variation to accommodate divergence in the evolving myocardial structure. Here we aimed at verifying if a microstructured bioartificial scaffold alone can provoke an effect on stem cell behavior. To this purpose, we fabricated microstructured bioartificial polymeric constructs made of PLGA/gelatin mimicking anisotropic structure and mechanical properties of the myocardium. We found that PLGA/gelatin scaffolds promoted adhesion, elongation, ordered disposition, and early myocardial commitment of human mesenchymal stem cells suggesting that these constructs are able to crosstalk with stem cells in a precise and controlled manner. At the same time, the biomaterial degradation kinetics renders the PLGA/gelatin constructs very attractive for myocardial regeneration approaches. |
| format | Article |
| id | doaj-art-ae37699461504198a0e52e61d6f652ed |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-ae37699461504198a0e52e61d6f652ed2025-02-03T01:12:11ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/71761547176154Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In VitroCaterina Cristallini0Elisa Cibrario Rocchietti1Mariacristina Gagliardi2Leonardo Mortati3Silvia Saviozzi4Elena Bellotti5Valentina Turinetto6Maria Paola Sassi7Niccoletta Barbani8Claudia Giachino9Institute for Chemical-Physical Processes, IPCF C.N.R., UOS Pisa, 56122 Pisa, ItalyDepartment of Clinical and Biological Sciences, University of Turin, Orbassano, 10043 Turin, ItalyCenter for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, ItalyNational Institute of Research in Metrology, INRIM, 10135 Turin, ItalyDepartment of Clinical and Biological Sciences, University of Turin, Orbassano, 10043 Turin, ItalyDepartment of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, ItalyDepartment of Clinical and Biological Sciences, University of Turin, Orbassano, 10043 Turin, ItalyNational Institute of Research in Metrology, INRIM, 10135 Turin, ItalyDepartment of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, ItalyDepartment of Clinical and Biological Sciences, University of Turin, Orbassano, 10043 Turin, ItalyThe biomaterial scaffold plays a key role in most tissue engineering strategies. Its surface properties, micropatterning, degradation, and mechanical features affect not only the generation of the tissue construct in vitro, but also its in vivo functionality. The area of myocardial tissue engineering still faces significant difficulties and challenges in the design of bioactive scaffolds, which allow composition variation to accommodate divergence in the evolving myocardial structure. Here we aimed at verifying if a microstructured bioartificial scaffold alone can provoke an effect on stem cell behavior. To this purpose, we fabricated microstructured bioartificial polymeric constructs made of PLGA/gelatin mimicking anisotropic structure and mechanical properties of the myocardium. We found that PLGA/gelatin scaffolds promoted adhesion, elongation, ordered disposition, and early myocardial commitment of human mesenchymal stem cells suggesting that these constructs are able to crosstalk with stem cells in a precise and controlled manner. At the same time, the biomaterial degradation kinetics renders the PLGA/gelatin constructs very attractive for myocardial regeneration approaches.http://dx.doi.org/10.1155/2016/7176154 |
| spellingShingle | Caterina Cristallini Elisa Cibrario Rocchietti Mariacristina Gagliardi Leonardo Mortati Silvia Saviozzi Elena Bellotti Valentina Turinetto Maria Paola Sassi Niccoletta Barbani Claudia Giachino Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro Stem Cells International |
| title | Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro |
| title_full | Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro |
| title_fullStr | Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro |
| title_full_unstemmed | Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro |
| title_short | Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro |
| title_sort | micro and macrostructured plga gelatin scaffolds promote early cardiogenic commitment of human mesenchymal stem cells in vitro |
| url | http://dx.doi.org/10.1155/2016/7176154 |
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