Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic Chinese
Pathological myopia is a severe hereditary ocular disease leading to blindness. It is urgent and very important to find the pathogenesis and therapy for this disease. The purpose of the study is to analyze sequences of lumican and decorin genes with pathological myopia(PM) and control subjects to ve...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2009-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2009/514306 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561867097112576 |
|---|---|
| author | Fengju Zhang Tingzhun Zhu Zhongjun Zhou Yudong Wu Yang Li |
| author_facet | Fengju Zhang Tingzhun Zhu Zhongjun Zhou Yudong Wu Yang Li |
| author_sort | Fengju Zhang |
| collection | DOAJ |
| description | Pathological myopia is a severe hereditary ocular disease leading to blindness. It is urgent and very important to find the pathogenesis and therapy for this disease. The purpose of the study is to analyze sequences of lumican and decorin genes with pathological myopia(PM) and control subjects to verify the relationship between lumican, decorin genes and PM in Northern Han Chinese. We collected and analyzed the blood samples of 94 adults (including 12 pedigree cases and 82 sporadic cases) with PM and 90 controls in the northern Han ethnic Chinese. Genotyping was performed by direct sequencing after polymerase chain reaction(PCR) amplification and allele frequencies were tested for Hardy-Weinberg equilibrium. Univariate analysis revealed significant differences between two groups for three SNPs: rs3759223 (C→T) and rs17853500 (T→C) of the lumican gene and rs74419 (T→C) of decorin gene with (P<.05) for all their genotype distribution and allele frequency. There is no significant difference for incidence of these mutations between pedigree and sporadic group (P>.05). The results suggested that the sequence variants in 5′-regulatory region of lumican gene and 3'UTR of decorin gene were associated significantly with PM in Northern Han Chinese. Further studies are needed to confirm finally whether the two genes are the virulence genes of PM. |
| format | Article |
| id | doaj-art-ae1f999fc5ea49499f4a19d0cd475656 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-ae1f999fc5ea49499f4a19d0cd4756562025-02-03T01:24:01ZengWileyJournal of Ophthalmology2090-004X2090-00582009-01-01200910.1155/2009/514306514306Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic ChineseFengju Zhang0Tingzhun Zhu1Zhongjun Zhou2Yudong Wu3Yang Li4Eye Center of Beijing Tongren Hospital of Capital Medical University, Beijing 100730, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, ChinaDepartment of Biochemistry, Hongkong University, Hong KongEye Center of Beijing Tongren Hospital of Capital Medical University, Beijing 100730, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, ChinaPathological myopia is a severe hereditary ocular disease leading to blindness. It is urgent and very important to find the pathogenesis and therapy for this disease. The purpose of the study is to analyze sequences of lumican and decorin genes with pathological myopia(PM) and control subjects to verify the relationship between lumican, decorin genes and PM in Northern Han Chinese. We collected and analyzed the blood samples of 94 adults (including 12 pedigree cases and 82 sporadic cases) with PM and 90 controls in the northern Han ethnic Chinese. Genotyping was performed by direct sequencing after polymerase chain reaction(PCR) amplification and allele frequencies were tested for Hardy-Weinberg equilibrium. Univariate analysis revealed significant differences between two groups for three SNPs: rs3759223 (C→T) and rs17853500 (T→C) of the lumican gene and rs74419 (T→C) of decorin gene with (P<.05) for all their genotype distribution and allele frequency. There is no significant difference for incidence of these mutations between pedigree and sporadic group (P>.05). The results suggested that the sequence variants in 5′-regulatory region of lumican gene and 3'UTR of decorin gene were associated significantly with PM in Northern Han Chinese. Further studies are needed to confirm finally whether the two genes are the virulence genes of PM.http://dx.doi.org/10.1155/2009/514306 |
| spellingShingle | Fengju Zhang Tingzhun Zhu Zhongjun Zhou Yudong Wu Yang Li Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic Chinese Journal of Ophthalmology |
| title | Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic Chinese |
| title_full | Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic Chinese |
| title_fullStr | Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic Chinese |
| title_full_unstemmed | Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic Chinese |
| title_short | Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic Chinese |
| title_sort | association of lumican gene with susceptibility to pathological myopia in the northern han ethnic chinese |
| url | http://dx.doi.org/10.1155/2009/514306 |
| work_keys_str_mv | AT fengjuzhang associationoflumicangenewithsusceptibilitytopathologicalmyopiainthenorthernhanethnicchinese AT tingzhunzhu associationoflumicangenewithsusceptibilitytopathologicalmyopiainthenorthernhanethnicchinese AT zhongjunzhou associationoflumicangenewithsusceptibilitytopathologicalmyopiainthenorthernhanethnicchinese AT yudongwu associationoflumicangenewithsusceptibilitytopathologicalmyopiainthenorthernhanethnicchinese AT yangli associationoflumicangenewithsusceptibilitytopathologicalmyopiainthenorthernhanethnicchinese |