Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate Gyrus
Long-term recognition memory requires protein synthesis, but little is known about the coordinate regulation of specific genes. Here, we examined expression of the plasticity-associated immediate early genes (Arc, Zif268, and Narp) in the dentate gyrus following long-term object-place recognition le...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2008/269097 |
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| _version_ | 1832548778879483904 |
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| author | Jonathan Soulé Zsuzsa Penke Tambudzai Kanhema Maria Nordheim Alme Serge Laroche Clive R. Bramham |
| author_facet | Jonathan Soulé Zsuzsa Penke Tambudzai Kanhema Maria Nordheim Alme Serge Laroche Clive R. Bramham |
| author_sort | Jonathan Soulé |
| collection | DOAJ |
| description | Long-term recognition memory requires protein synthesis, but little is known about the coordinate regulation of specific genes. Here, we examined expression of the plasticity-associated immediate early genes (Arc, Zif268, and Narp) in the dentate gyrus following long-term object-place recognition learning in rats. RT-PCR analysis from dentate gyrus tissue collected shortly after training did not reveal learning-specific changes in Arc mRNA expression. In situ hybridization and immunohistochemistry were therefore used to assess possible sparse effects on gene expression. Learning about objects increased the density of granule cells expressing Arc, and to a lesser extent Narp, specifically in the dorsal blade of the dentate gyrus, while Zif268 expression was elevated across both blades. Thus, object-place recognition triggers rapid, blade-specific upregulation of plasticity-associated immediate early genes. Furthermore, Western blot analysis of dentate gyrus homogenates demonstrated concomitant upregulation of three postsynaptic density proteins (Arc, PSD-95, and α-CaMKII) with key roles in long-term synaptic plasticity and long-term memory. |
| format | Article |
| id | doaj-art-adce4e4616f640e588455837dc7aa225 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-adce4e4616f640e588455837dc7aa2252025-02-03T06:13:09ZengWileyNeural Plasticity2090-59041687-54432008-01-01200810.1155/2008/269097269097Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate GyrusJonathan Soulé0Zsuzsa Penke1Tambudzai Kanhema2Maria Nordheim Alme3Serge Laroche4Clive R. Bramham5Department of Biomedicine and Bergen Mental Health Research Center, University of Bergen, Jonas Lies vei 91, 5009 Bergen, NorwayCNRS, Université Paris-Sud, UMR 8620, Laboratoire de Neurobiologie de l’Apprentissage, de la Mémoire et de la Communication, 91405 Orsay Cedex, FranceDepartment of Biomedicine and Bergen Mental Health Research Center, University of Bergen, Jonas Lies vei 91, 5009 Bergen, NorwayDepartment of Biomedicine and Bergen Mental Health Research Center, University of Bergen, Jonas Lies vei 91, 5009 Bergen, NorwayCNRS, Université Paris-Sud, UMR 8620, Laboratoire de Neurobiologie de l’Apprentissage, de la Mémoire et de la Communication, 91405 Orsay Cedex, FranceDepartment of Biomedicine and Bergen Mental Health Research Center, University of Bergen, Jonas Lies vei 91, 5009 Bergen, NorwayLong-term recognition memory requires protein synthesis, but little is known about the coordinate regulation of specific genes. Here, we examined expression of the plasticity-associated immediate early genes (Arc, Zif268, and Narp) in the dentate gyrus following long-term object-place recognition learning in rats. RT-PCR analysis from dentate gyrus tissue collected shortly after training did not reveal learning-specific changes in Arc mRNA expression. In situ hybridization and immunohistochemistry were therefore used to assess possible sparse effects on gene expression. Learning about objects increased the density of granule cells expressing Arc, and to a lesser extent Narp, specifically in the dorsal blade of the dentate gyrus, while Zif268 expression was elevated across both blades. Thus, object-place recognition triggers rapid, blade-specific upregulation of plasticity-associated immediate early genes. Furthermore, Western blot analysis of dentate gyrus homogenates demonstrated concomitant upregulation of three postsynaptic density proteins (Arc, PSD-95, and α-CaMKII) with key roles in long-term synaptic plasticity and long-term memory.http://dx.doi.org/10.1155/2008/269097 |
| spellingShingle | Jonathan Soulé Zsuzsa Penke Tambudzai Kanhema Maria Nordheim Alme Serge Laroche Clive R. Bramham Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate Gyrus Neural Plasticity |
| title | Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate Gyrus |
| title_full | Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate Gyrus |
| title_fullStr | Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate Gyrus |
| title_full_unstemmed | Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate Gyrus |
| title_short | Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate Gyrus |
| title_sort | object place recognition learning triggers rapid induction of plasticity related immediate early genes and synaptic proteins in the rat dentate gyrus |
| url | http://dx.doi.org/10.1155/2008/269097 |
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