Screening of <em>FecLL</em> prolific allele of the <em>B4GALNT2</em> gene in Algerian sheep populations
Background: Research on the main genes controlling prolificacy in sheep, also known as fecundity genes (Fec genes), has highlighted the mechanisms involved in ewe fertility and the genetic improvement of prolificacy. The gene, FecL/B4GALNT2 (beta-1,4-N-acetyl-galactosaminyl transferase 2) and its...
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CIRAD
2024-07-01
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| Series: | Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux |
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| Online Access: | https://revues.cirad.fr/index.php/REMVT/article/view/37433 |
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| author | Assia Hadjazi Fatima Zahra Belharfi Fatima Zohra Mahammi Stéphane Fabre Semir Bechir Suheil Gaouar Nacera Tabet-Aoul |
| author_facet | Assia Hadjazi Fatima Zahra Belharfi Fatima Zohra Mahammi Stéphane Fabre Semir Bechir Suheil Gaouar Nacera Tabet-Aoul |
| author_sort | Assia Hadjazi |
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Background: Research on the main genes controlling prolificacy in sheep, also known as fecundity genes (Fec genes), has highlighted the mechanisms involved in ewe fertility and the genetic improvement of prolificacy. The gene, FecL/B4GALNT2 (beta-1,4-N-acetyl-galactosaminyl transferase 2) and its prolific allele FecLL, which segregate in French Lacaune sheep and some local sheep breeds in Morocco and Tunisia, were found to influence ovarian function. However, their action differs from that of other major genes discovered previously, such as: FecX/BMP15 (bone morphogenetic protein 15); FecG/GDF9 (growth differentiation factor 9); and FecB/BMPR1B (bone morphogenetic protein receptor type 1B). The latter act in the bone morphogenetic protein (BMP) signalling pathway. Aim: The objective of this study is to investigate the segregation of the FecLL allele in Algerian sheep populations. Methods: A total of 338 animals from 12 breeds were genotyped using the PCR-RFLP technique. Results: Our results revealed the presence of FecLL in the Algerian D’man sheep population. Among the genotyped D’man sheep, 21% were carriers of the mutation in the heterozygous state. The frequency of the FecLL allele in the Algerian D’man population (0.11) is close to what is observed in Lacaune sheep and remains relatively low compared to Moroccan (0.58) and Tunisian (0.65) D’man sheep. Conclusions: The FecLL allele, which is shared by the French Lacaune population and the North African D’man populations, could indicate the ancestral origin of the mutation in B4GALNT2 or the occurrence of an ancient introgression event to improve prolificacy. Managing this mutation in Algerian D’man flocks could help improve the numerical productivity of D’man sheep in Algeria.
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| format | Article |
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| institution | Kabale University |
| issn | 0035-1865 1951-6711 |
| language | English |
| publishDate | 2024-07-01 |
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| series | Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux |
| spelling | doaj-art-adc9c7d400714a269d16324d2ac18fcf2025-01-23T13:28:03ZengCIRADRevue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux0035-18651951-67112024-07-017710.19182/remvt.37433Screening of <em>FecLL</em> prolific allele of the <em>B4GALNT2</em> gene in Algerian sheep populationsAssia Hadjazi0Fatima Zahra Belharfi1Fatima Zohra Mahammi2Stéphane Fabre3Semir Bechir Suheil Gaouar4Nacera Tabet-Aoul5Laboratoire de Génétique Moléculaire et Cellulaire, Département de Génétique Moléculaire Appliquée, Faculté des Sciences de la Nature et de la Vie, Université des Sciences et de la Technologie d’Oran Mohamed Boudiaf, Oran, AlgeriaLaboratoire de Génétique Appliquée en Agronomie, Ecologie et Santé publique (GenApAgiE), Département de biologie, Faculté SNV/STU, Université Abou Bekr Belkaid, Tlemcen, AlgeriaLaboratoire de Génétique Moléculaire et Cellulaire, Département de Génétique Moléculaire Appliquée, Faculté des Sciences de la Nature et de la Vie, Université des Sciences et de la Technologie d’Oran Mohamed Boudiaf, Oran, Algeria ; École Supérieure en Sciences Biologiques d’Oran (ESSBO), Oran, AlgeriaLaboratoire GenPhySE, INRAE Occitanie Toulouse, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, Centre Occitanie, Toulouse, FranceLaboratoire de Génétique Appliquée en Agronomie, Ecologie et Santé publique (GenApAgiE), Département de biologie, Faculté SNV/STU, Université Abou Bekr Belkaid, Tlemcen, AlgeriaLaboratoire de Génétique Moléculaire et Cellulaire, Département de Génétique Moléculaire Appliquée, Faculté des Sciences de la Nature et de la Vie, Université des Sciences et de la Technologie d’Oran Mohamed Boudiaf, Oran, Algeria ; Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université Oran 1 Background: Research on the main genes controlling prolificacy in sheep, also known as fecundity genes (Fec genes), has highlighted the mechanisms involved in ewe fertility and the genetic improvement of prolificacy. The gene, FecL/B4GALNT2 (beta-1,4-N-acetyl-galactosaminyl transferase 2) and its prolific allele FecLL, which segregate in French Lacaune sheep and some local sheep breeds in Morocco and Tunisia, were found to influence ovarian function. However, their action differs from that of other major genes discovered previously, such as: FecX/BMP15 (bone morphogenetic protein 15); FecG/GDF9 (growth differentiation factor 9); and FecB/BMPR1B (bone morphogenetic protein receptor type 1B). The latter act in the bone morphogenetic protein (BMP) signalling pathway. Aim: The objective of this study is to investigate the segregation of the FecLL allele in Algerian sheep populations. Methods: A total of 338 animals from 12 breeds were genotyped using the PCR-RFLP technique. Results: Our results revealed the presence of FecLL in the Algerian D’man sheep population. Among the genotyped D’man sheep, 21% were carriers of the mutation in the heterozygous state. The frequency of the FecLL allele in the Algerian D’man population (0.11) is close to what is observed in Lacaune sheep and remains relatively low compared to Moroccan (0.58) and Tunisian (0.65) D’man sheep. Conclusions: The FecLL allele, which is shared by the French Lacaune population and the North African D’man populations, could indicate the ancestral origin of the mutation in B4GALNT2 or the occurrence of an ancient introgression event to improve prolificacy. Managing this mutation in Algerian D’man flocks could help improve the numerical productivity of D’man sheep in Algeria. https://revues.cirad.fr/index.php/REMVT/article/view/37433Sheepgenotypesgenetic polymorphismproductivitylandracesAlgeria |
| spellingShingle | Assia Hadjazi Fatima Zahra Belharfi Fatima Zohra Mahammi Stéphane Fabre Semir Bechir Suheil Gaouar Nacera Tabet-Aoul Screening of <em>FecLL</em> prolific allele of the <em>B4GALNT2</em> gene in Algerian sheep populations Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux Sheep genotypes genetic polymorphism productivity landraces Algeria |
| title | Screening of <em>FecLL</em> prolific allele of the <em>B4GALNT2</em> gene in Algerian sheep populations |
| title_full | Screening of <em>FecLL</em> prolific allele of the <em>B4GALNT2</em> gene in Algerian sheep populations |
| title_fullStr | Screening of <em>FecLL</em> prolific allele of the <em>B4GALNT2</em> gene in Algerian sheep populations |
| title_full_unstemmed | Screening of <em>FecLL</em> prolific allele of the <em>B4GALNT2</em> gene in Algerian sheep populations |
| title_short | Screening of <em>FecLL</em> prolific allele of the <em>B4GALNT2</em> gene in Algerian sheep populations |
| title_sort | screening of em fecll em prolific allele of the em b4galnt2 em gene in algerian sheep populations |
| topic | Sheep genotypes genetic polymorphism productivity landraces Algeria |
| url | https://revues.cirad.fr/index.php/REMVT/article/view/37433 |
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