Flaxseed Extract Can Modulates Apoptotic Genes to Promote Cell Death in Breast Cancer Cells
Background: Breast cancer is a multifactorial malignancy with uncertain treatment outcomes, making its treatment challenging. Balanced level of genes expression, including BAX, BCL2L1 (bcl-xl) and BCL-2 are needed to regulate apoptosis pathway in cancers. The aim of this study was to examine the imp...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2025-07-01
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| Series: | Middle East Journal of Cancer |
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| Online Access: | https://mejc.sums.ac.ir/article_50550_f3f60b502efc7552c80760dbbe392959.pdf |
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| author | Ehsan Zare Mehrjardi Shadi Montazerolhojeh Masoud Dehghan Tezerjani Safieh Dehshiri Mahmood Dehghani Ashkezari Seyed Morteza Seifati |
| author_facet | Ehsan Zare Mehrjardi Shadi Montazerolhojeh Masoud Dehghan Tezerjani Safieh Dehshiri Mahmood Dehghani Ashkezari Seyed Morteza Seifati |
| author_sort | Ehsan Zare Mehrjardi |
| collection | DOAJ |
| description | Background: Breast cancer is a multifactorial malignancy with uncertain treatment outcomes, making its treatment challenging. Balanced level of genes expression, including BAX, BCL2L1 (bcl-xl) and BCL-2 are needed to regulate apoptosis pathway in cancers. The aim of this study was to examine the impact of flaxseed extract on the expression of BAX, BCL-2, and BCL2L1 (bcl-xl) gens in BT-474 and MCF-7 breast cancer cells.Method: This in vitro experimental study investigated the impact of flaxseed extract on apoptosis and the proliferation of BT-474 and MCF-7 breast cancer cells. MTT assay was conducted on flaxseed extract treated BT-474 and MCF-7 cells at concentrations 3.90628, 7.8125, 156.125, 312.5, 125, 2, 1000, and 2000 µg/ml for 24, 48 and 72h. IC50 (μg/ml) was calculated for flaxseed extract in both cells. The expression of BCL2L1 (bcl-xl), bcl-2 and Bax genes were evaluated at these concentrations using real time Polymerase Chain Reaction (PCR). The data from different groups were analyzed using the Student's t-test.Results: Flaxseed extract reduced the multiplication and growth of BT-474 and MCF-7 cells in a concentration-dependent manner over 24, 48, and 72 hours of incubation. In both flaxseed-extract exposed BT-474 and MCF-7 cell lines, the BAX gene expression increased (P < 0.05); however, the gene expression of BCL2L1 (bcl-xl) and BCL-2 genes decreased (P < 0.05).Conclusion: Incubating BT-474 and MCF-7 with flaxseed extract repressed their growth and induced apoptosis. These results may provide precious information for developing a plant-based agent to prevent or treat breast cancer. |
| format | Article |
| id | doaj-art-adc4e8a8d2b54044807b6516e6fc37f2 |
| institution | Kabale University |
| issn | 2008-6709 2008-6687 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Middle East Journal of Cancer |
| spelling | doaj-art-adc4e8a8d2b54044807b6516e6fc37f22025-08-20T03:34:13ZengShiraz University of Medical SciencesMiddle East Journal of Cancer2008-67092008-66872025-07-0116325826610.30476/mejc.2024.102754.210850550Flaxseed Extract Can Modulates Apoptotic Genes to Promote Cell Death in Breast Cancer CellsEhsan Zare Mehrjardi0Shadi Montazerolhojeh1Masoud Dehghan Tezerjani2Safieh Dehshiri3Mahmood Dehghani Ashkezari4Seyed Morteza Seifati5Department of Industrial Environmental and Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranMedical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, IranAbortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, IranMedical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, IranMedical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, IranMedical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, IranBackground: Breast cancer is a multifactorial malignancy with uncertain treatment outcomes, making its treatment challenging. Balanced level of genes expression, including BAX, BCL2L1 (bcl-xl) and BCL-2 are needed to regulate apoptosis pathway in cancers. The aim of this study was to examine the impact of flaxseed extract on the expression of BAX, BCL-2, and BCL2L1 (bcl-xl) gens in BT-474 and MCF-7 breast cancer cells.Method: This in vitro experimental study investigated the impact of flaxseed extract on apoptosis and the proliferation of BT-474 and MCF-7 breast cancer cells. MTT assay was conducted on flaxseed extract treated BT-474 and MCF-7 cells at concentrations 3.90628, 7.8125, 156.125, 312.5, 125, 2, 1000, and 2000 µg/ml for 24, 48 and 72h. IC50 (μg/ml) was calculated for flaxseed extract in both cells. The expression of BCL2L1 (bcl-xl), bcl-2 and Bax genes were evaluated at these concentrations using real time Polymerase Chain Reaction (PCR). The data from different groups were analyzed using the Student's t-test.Results: Flaxseed extract reduced the multiplication and growth of BT-474 and MCF-7 cells in a concentration-dependent manner over 24, 48, and 72 hours of incubation. In both flaxseed-extract exposed BT-474 and MCF-7 cell lines, the BAX gene expression increased (P < 0.05); however, the gene expression of BCL2L1 (bcl-xl) and BCL-2 genes decreased (P < 0.05).Conclusion: Incubating BT-474 and MCF-7 with flaxseed extract repressed their growth and induced apoptosis. These results may provide precious information for developing a plant-based agent to prevent or treat breast cancer.https://mejc.sums.ac.ir/article_50550_f3f60b502efc7552c80760dbbe392959.pdfbreast neoplasmsapoptosisplant extractsic50cell line |
| spellingShingle | Ehsan Zare Mehrjardi Shadi Montazerolhojeh Masoud Dehghan Tezerjani Safieh Dehshiri Mahmood Dehghani Ashkezari Seyed Morteza Seifati Flaxseed Extract Can Modulates Apoptotic Genes to Promote Cell Death in Breast Cancer Cells Middle East Journal of Cancer breast neoplasms apoptosis plant extracts ic50 cell line |
| title | Flaxseed Extract Can Modulates Apoptotic Genes to Promote Cell Death in Breast Cancer Cells |
| title_full | Flaxseed Extract Can Modulates Apoptotic Genes to Promote Cell Death in Breast Cancer Cells |
| title_fullStr | Flaxseed Extract Can Modulates Apoptotic Genes to Promote Cell Death in Breast Cancer Cells |
| title_full_unstemmed | Flaxseed Extract Can Modulates Apoptotic Genes to Promote Cell Death in Breast Cancer Cells |
| title_short | Flaxseed Extract Can Modulates Apoptotic Genes to Promote Cell Death in Breast Cancer Cells |
| title_sort | flaxseed extract can modulates apoptotic genes to promote cell death in breast cancer cells |
| topic | breast neoplasms apoptosis plant extracts ic50 cell line |
| url | https://mejc.sums.ac.ir/article_50550_f3f60b502efc7552c80760dbbe392959.pdf |
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