Global Transcriptomic Analysis of Inbred Lines Reveal Candidate Genes for Response to Maize Lethal Necrosis
Maize lethal necrosis (MLN) is a significant threat to food security in Sub-Saharan Africa (SSA), with limited commercial inbred lines displaying tolerance. This study analyzed the transcriptomes of four commercially used maize inbred lines and a non-adapted inbred line, all with varying response le...
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2025-01-01
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| author | Ann Murithi Gayathri Panangipalli Zhengyu Wen Michael S. Olsen Thomas Lübberstedt Kanwarpal S. Dhugga Mark Jung |
| author_facet | Ann Murithi Gayathri Panangipalli Zhengyu Wen Michael S. Olsen Thomas Lübberstedt Kanwarpal S. Dhugga Mark Jung |
| author_sort | Ann Murithi |
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| description | Maize lethal necrosis (MLN) is a significant threat to food security in Sub-Saharan Africa (SSA), with limited commercial inbred lines displaying tolerance. This study analyzed the transcriptomes of four commercially used maize inbred lines and a non-adapted inbred line, all with varying response levels to MLN. RNA-Seq revealed differentially expressed genes in response to infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV), the causative agents of MLN. Key findings included the identification of components of the plant innate immune system, such as differentially regulated R genes (mainly LRRs), and activation/deactivation of virus resistance pathways, including RNA interference (RNAi) via <i>Argonaute</i> (AGO), <i>Dicer-like proteins</i>, and the ubiquitin–proteasome system (UPS) via <i>RING/U-box</i> and <i>ubiquitin ligases</i>. Genes associated with redox signaling, <i>WRKY</i> transcription factors, and cell modification were also differentially expressed. Additionally, the expression of translation initiation and elongation factors, <i>eIF4E</i> and <i>eIF4G</i>, correlated with the presence of MLN viruses. These findings provide valuable insights into the molecular mechanisms of MLN resistance and highlight potential gene candidates for engineering or selecting MLN-resistant maize germplasm for SSA. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-ad6adb2a7ee242c98e1872d56586d8242025-01-24T13:47:09ZengMDPI AGPlants2223-77472025-01-0114229510.3390/plants14020295Global Transcriptomic Analysis of Inbred Lines Reveal Candidate Genes for Response to Maize Lethal NecrosisAnn Murithi0Gayathri Panangipalli1Zhengyu Wen2Michael S. Olsen3Thomas Lübberstedt4Kanwarpal S. Dhugga5Mark Jung6Corteva Agriscience, 7000 NW 62nd Ave, Johnston, IA 50131, USACorteva Agriscience, 7000 NW 62nd Ave, Johnston, IA 50131, USAInternational Maize and Wheat Improvement Center (CIMMYT), Carretera México-Veracruz, Km. 45, Texcoco 56237, MexicoBayer, Crop Science Division, 800 N. Lindbergh Blvd., St. Louis, MO 63167, USADepartment of Agronomy, Iowa State University, 716 Farm House Lane, Ames, IA 50011, USAInternational Maize and Wheat Improvement Center (CIMMYT), Carretera México-Veracruz, Km. 45, Texcoco 56237, MexicoCorteva Agriscience, 7000 NW 62nd Ave, Johnston, IA 50131, USAMaize lethal necrosis (MLN) is a significant threat to food security in Sub-Saharan Africa (SSA), with limited commercial inbred lines displaying tolerance. This study analyzed the transcriptomes of four commercially used maize inbred lines and a non-adapted inbred line, all with varying response levels to MLN. RNA-Seq revealed differentially expressed genes in response to infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV), the causative agents of MLN. Key findings included the identification of components of the plant innate immune system, such as differentially regulated R genes (mainly LRRs), and activation/deactivation of virus resistance pathways, including RNA interference (RNAi) via <i>Argonaute</i> (AGO), <i>Dicer-like proteins</i>, and the ubiquitin–proteasome system (UPS) via <i>RING/U-box</i> and <i>ubiquitin ligases</i>. Genes associated with redox signaling, <i>WRKY</i> transcription factors, and cell modification were also differentially expressed. Additionally, the expression of translation initiation and elongation factors, <i>eIF4E</i> and <i>eIF4G</i>, correlated with the presence of MLN viruses. These findings provide valuable insights into the molecular mechanisms of MLN resistance and highlight potential gene candidates for engineering or selecting MLN-resistant maize germplasm for SSA.https://www.mdpi.com/2223-7747/14/2/295maizevirusesMCMVSCMVMLNgene expression |
| spellingShingle | Ann Murithi Gayathri Panangipalli Zhengyu Wen Michael S. Olsen Thomas Lübberstedt Kanwarpal S. Dhugga Mark Jung Global Transcriptomic Analysis of Inbred Lines Reveal Candidate Genes for Response to Maize Lethal Necrosis Plants maize viruses MCMV SCMV MLN gene expression |
| title | Global Transcriptomic Analysis of Inbred Lines Reveal Candidate Genes for Response to Maize Lethal Necrosis |
| title_full | Global Transcriptomic Analysis of Inbred Lines Reveal Candidate Genes for Response to Maize Lethal Necrosis |
| title_fullStr | Global Transcriptomic Analysis of Inbred Lines Reveal Candidate Genes for Response to Maize Lethal Necrosis |
| title_full_unstemmed | Global Transcriptomic Analysis of Inbred Lines Reveal Candidate Genes for Response to Maize Lethal Necrosis |
| title_short | Global Transcriptomic Analysis of Inbred Lines Reveal Candidate Genes for Response to Maize Lethal Necrosis |
| title_sort | global transcriptomic analysis of inbred lines reveal candidate genes for response to maize lethal necrosis |
| topic | maize viruses MCMV SCMV MLN gene expression |
| url | https://www.mdpi.com/2223-7747/14/2/295 |
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