Effect of Phosphate Starvation on Gene Expression in <i>Komagataella phaffii</i> Cells
Phosphorus is a key nutrient for all organisms. The study of phosphate metabolism and its regulation is important for understanding the evolutionary processes of regulatory systems in eukaryotic cells. The methylotrophic yeast <i>Komagataella phaffii</i> is an efficient producer organism...
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2024-12-01
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| author | Valeria V. Ishtuganova Anton V. Sidorin Anastasiya S. Makeeva Marina V. Padkina Andrey M. Rumyantsev |
| author_facet | Valeria V. Ishtuganova Anton V. Sidorin Anastasiya S. Makeeva Marina V. Padkina Andrey M. Rumyantsev |
| author_sort | Valeria V. Ishtuganova |
| collection | DOAJ |
| description | Phosphorus is a key nutrient for all organisms. The study of phosphate metabolism and its regulation is important for understanding the evolutionary processes of regulatory systems in eukaryotic cells. The methylotrophic yeast <i>Komagataella phaffii</i> is an efficient producer organism, and it is actively used in biotechnological production. The high practical importance of <i>K. phaffii</i> has stimulated active research to find new tools to work with this yeast and optimize its cultivation conditions. In this work, we observed the effect of phosphate starvation on gene expression in <i>K. phaffii</i> at the transcriptome level. Phosphate starvation had a significant effect on general cell metabolism. <i>K. phaffii</i> cells demonstrated a response to this macronutrient deficiency through an altered gene expression of carbon and amino acid metabolism. We observed the activation of phosphate and polyphosphate metabolism gene expression. In this case, there was a suppression of ribosome biogenesis genes and genes involved in fatty acid beta-oxidation and translation processes. |
| format | Article |
| id | doaj-art-ad50d9cfb84447b1aa9e0db92a89f28d |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-ad50d9cfb84447b1aa9e0db92a89f28d2025-01-24T13:42:23ZengMDPI AGMicroorganisms2076-26072024-12-011313910.3390/microorganisms13010039Effect of Phosphate Starvation on Gene Expression in <i>Komagataella phaffii</i> CellsValeria V. Ishtuganova0Anton V. Sidorin1Anastasiya S. Makeeva2Marina V. Padkina3Andrey M. Rumyantsev4Department of Genetics and Biotechnology, Saint Petersburg State University, 199034 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 199034 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 199034 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 199034 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 199034 St. Petersburg, RussiaPhosphorus is a key nutrient for all organisms. The study of phosphate metabolism and its regulation is important for understanding the evolutionary processes of regulatory systems in eukaryotic cells. The methylotrophic yeast <i>Komagataella phaffii</i> is an efficient producer organism, and it is actively used in biotechnological production. The high practical importance of <i>K. phaffii</i> has stimulated active research to find new tools to work with this yeast and optimize its cultivation conditions. In this work, we observed the effect of phosphate starvation on gene expression in <i>K. phaffii</i> at the transcriptome level. Phosphate starvation had a significant effect on general cell metabolism. <i>K. phaffii</i> cells demonstrated a response to this macronutrient deficiency through an altered gene expression of carbon and amino acid metabolism. We observed the activation of phosphate and polyphosphate metabolism gene expression. In this case, there was a suppression of ribosome biogenesis genes and genes involved in fatty acid beta-oxidation and translation processes.https://www.mdpi.com/2076-2607/13/1/39<i>Komagataella phaffii</i>phosphorustranscriptomic analysisdifferential gene expression |
| spellingShingle | Valeria V. Ishtuganova Anton V. Sidorin Anastasiya S. Makeeva Marina V. Padkina Andrey M. Rumyantsev Effect of Phosphate Starvation on Gene Expression in <i>Komagataella phaffii</i> Cells Microorganisms <i>Komagataella phaffii</i> phosphorus transcriptomic analysis differential gene expression |
| title | Effect of Phosphate Starvation on Gene Expression in <i>Komagataella phaffii</i> Cells |
| title_full | Effect of Phosphate Starvation on Gene Expression in <i>Komagataella phaffii</i> Cells |
| title_fullStr | Effect of Phosphate Starvation on Gene Expression in <i>Komagataella phaffii</i> Cells |
| title_full_unstemmed | Effect of Phosphate Starvation on Gene Expression in <i>Komagataella phaffii</i> Cells |
| title_short | Effect of Phosphate Starvation on Gene Expression in <i>Komagataella phaffii</i> Cells |
| title_sort | effect of phosphate starvation on gene expression in i komagataella phaffii i cells |
| topic | <i>Komagataella phaffii</i> phosphorus transcriptomic analysis differential gene expression |
| url | https://www.mdpi.com/2076-2607/13/1/39 |
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