Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential
Mesenchymal stem cells (MSCs) are effective therapeutic agents that contribute to tissue repair and regeneration by secreting various factors. However, donor-dependent variations in MSC proliferation and therapeutic potentials result in variable production yields and clinical outcomes, thereby imped...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/4711499 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548345526091776 |
|---|---|
| author | Sun Jeong Kim Sang Eon Park Jang Bin Jeong Shin Ji Oh Alee Choi Yun Hee Kim Suk-joo Choi Soo-young Oh Gyu Ha Ryu Hong Bae Jeon Jong Wook Chang |
| author_facet | Sun Jeong Kim Sang Eon Park Jang Bin Jeong Shin Ji Oh Alee Choi Yun Hee Kim Suk-joo Choi Soo-young Oh Gyu Ha Ryu Hong Bae Jeon Jong Wook Chang |
| author_sort | Sun Jeong Kim |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are effective therapeutic agents that contribute to tissue repair and regeneration by secreting various factors. However, donor-dependent variations in MSC proliferation and therapeutic potentials result in variable production yields and clinical outcomes, thereby impeding MSC-based therapies. Hence, selection of MSCs with high proliferation and therapeutic potentials would be important for effective clinical application of MSCs. This study is aimed at identifying the upregulated genes in human Wharton’s jelly-derived MSCs (WJ-MSCs) with high proliferation potential using mRNA sequencing. Aurora kinase A (AURKA) and dedicator of cytokinesis 2 (DOCK2) were selected as the upregulated genes, and their effects on proliferation, migration, and colony formation of the WJ-MSCs were verified using small interfering RNA (siRNA) techniques. mRNA expression levels of both the genes were positively correlated with the proliferation capacity of WJ-MSCs. Moreover, AURKA from human WJ-MSCs regulated the antiapoptotic effect of skeletal muscle cells by upregulating the chemokine (C motif) ligand (XCL1); this was further confirmed in the mdx mouse model. Taken together, the results indicated that AURKA and DOCK2 can be used as potential biomarkers for proliferation and migration of human WJ-MSCs. In particular, human WJ-MSCs with high expression of AURKA might have therapeutic efficacy against muscle diseases, such as Duchenne muscular dystrophy (DMD). |
| format | Article |
| id | doaj-art-acf4e35b797c4df39fe1246d49f773c3 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-acf4e35b797c4df39fe1246d49f773c32025-02-03T06:14:20ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/4711499Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic PotentialSun Jeong Kim0Sang Eon Park1Jang Bin Jeong2Shin Ji Oh3Alee Choi4Yun Hee Kim5Suk-joo Choi6Soo-young Oh7Gyu Ha Ryu8Hong Bae Jeon9Jong Wook Chang10Stem Cell InstituteStem Cell InstituteStem Cell InstituteStem Cell InstituteStem Cell InstituteStem Cell InstituteDepartment of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyDepartment of Medical Device Management and ResearchStem Cell InstituteStem Cell InstituteMesenchymal stem cells (MSCs) are effective therapeutic agents that contribute to tissue repair and regeneration by secreting various factors. However, donor-dependent variations in MSC proliferation and therapeutic potentials result in variable production yields and clinical outcomes, thereby impeding MSC-based therapies. Hence, selection of MSCs with high proliferation and therapeutic potentials would be important for effective clinical application of MSCs. This study is aimed at identifying the upregulated genes in human Wharton’s jelly-derived MSCs (WJ-MSCs) with high proliferation potential using mRNA sequencing. Aurora kinase A (AURKA) and dedicator of cytokinesis 2 (DOCK2) were selected as the upregulated genes, and their effects on proliferation, migration, and colony formation of the WJ-MSCs were verified using small interfering RNA (siRNA) techniques. mRNA expression levels of both the genes were positively correlated with the proliferation capacity of WJ-MSCs. Moreover, AURKA from human WJ-MSCs regulated the antiapoptotic effect of skeletal muscle cells by upregulating the chemokine (C motif) ligand (XCL1); this was further confirmed in the mdx mouse model. Taken together, the results indicated that AURKA and DOCK2 can be used as potential biomarkers for proliferation and migration of human WJ-MSCs. In particular, human WJ-MSCs with high expression of AURKA might have therapeutic efficacy against muscle diseases, such as Duchenne muscular dystrophy (DMD).http://dx.doi.org/10.1155/2022/4711499 |
| spellingShingle | Sun Jeong Kim Sang Eon Park Jang Bin Jeong Shin Ji Oh Alee Choi Yun Hee Kim Suk-joo Choi Soo-young Oh Gyu Ha Ryu Hong Bae Jeon Jong Wook Chang Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential Stem Cells International |
| title | Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential |
| title_full | Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential |
| title_fullStr | Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential |
| title_full_unstemmed | Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential |
| title_short | Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential |
| title_sort | wharton s jelly derived mesenchymal stem cells with high aurora kinase a expression show improved proliferation migration and therapeutic potential |
| url | http://dx.doi.org/10.1155/2022/4711499 |
| work_keys_str_mv | AT sunjeongkim whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential AT sangeonpark whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential AT jangbinjeong whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential AT shinjioh whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential AT aleechoi whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential AT yunheekim whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential AT sukjoochoi whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential AT sooyoungoh whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential AT gyuharyu whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential AT hongbaejeon whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential AT jongwookchang whartonsjellyderivedmesenchymalstemcellswithhighaurorakinaseaexpressionshowimprovedproliferationmigrationandtherapeuticpotential |