Effect of Fish Meal Substitution with Black Soldier Fly (Hermetia illucens) on Growth Performance, Feed Stability, Blood Biochemistry, and Liver and Gut Morphology of Siamese Fighting Fish (Betta splendens)
Insects such as black soldier fly larvae (BSFL) are gaining interest among researchers and the aquafeed industry due to the fluctuating price and supply of fish meal (FM). This study evaluated the growth performance, feed stability, blood biochemistry, and liver and gut morphology of Betta splendens...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/2023/6676953 |
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| author | Zulhisyam Abdul Kari Guillermo Téllez-Isaías Noor Khalidah Abdul Hamid Nor Dini Rusli Khairiyah Mat Suniza Anis Mohamad Sukri Muhammad Anamul Kabir Ahmad Razali Ishak Nazri Che Dom Abdel-Wahab A. Abdel-Warith Elsayed M. Younis Martina Irwan Khoo Faizuan Abdullah Md Shahjahan Md Fazle Rohani Simon J. Davies Lee Seong Wei |
| author_facet | Zulhisyam Abdul Kari Guillermo Téllez-Isaías Noor Khalidah Abdul Hamid Nor Dini Rusli Khairiyah Mat Suniza Anis Mohamad Sukri Muhammad Anamul Kabir Ahmad Razali Ishak Nazri Che Dom Abdel-Wahab A. Abdel-Warith Elsayed M. Younis Martina Irwan Khoo Faizuan Abdullah Md Shahjahan Md Fazle Rohani Simon J. Davies Lee Seong Wei |
| author_sort | Zulhisyam Abdul Kari |
| collection | DOAJ |
| description | Insects such as black soldier fly larvae (BSFL) are gaining interest among researchers and the aquafeed industry due to the fluctuating price and supply of fish meal (FM). This study evaluated the growth performance, feed stability, blood biochemistry, and liver and gut morphology of Betta splendens using BSFL as an alternative to FM. Five formulated diets were prepared: 0% BSFL, 6.5% BSFL, 13% BSFL, 19.5% BSFL, and 24.5% BSFL. The expansion rate, pellet durability index, floatability, bulk density, and water stability of the prepared feed have been assessed. Except for the diameter of the feed, all the parameters studied differed significantly (p<0.05) across the experimental diets. After 60 days, the fish fed with 13% BSFL had the highest final length, final weight, net weight gain, specific growth rate, weight gain, and gastrointestinal weight, with mean and standard deviation values of 3.97±0.43 cm, 3.95±0.1 g, 2.78±0.1 g, 4.63±0.17, 4.65±0.13, 237.26±7.9%, and 0.04±0.01 mg, respectively. Similar blood haematology and biochemical properties, including corpuscular volume, lymphocytes, white blood cells, red blood cells, haematocrit, albumin, and alkaline phosphatase, were the highest (p<0.05) in the 13% BSFL diet group compared to the other treatment groups. In addition, BSFL had a significant impact (p<0.05) on villus length, width, and crypt depth for the anterior and posterior guts of B. splendens. The 13% BSFL diet group had an intact epithelial barrier in the goblet cell arrangement and a well-organized villus structure and tunica muscularis, compared to the other treatment groups. Furthermore, the liver cell was altered with different BSFL inclusions; the 13% FM group demonstrated better nuclei and cytoplasm structure than the other treatment groups. In conclusion, replacing 13% FM with BSFL could improve the growth performance, blood parameters, and liver and intestine morphology of B. splendens, thus providing a promising alternative diet for ornamental freshwater fish. |
| format | Article |
| id | doaj-art-aced57792b8c46a6b00fc8f960736e9d |
| institution | Kabale University |
| issn | 1365-2095 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture Nutrition |
| spelling | doaj-art-aced57792b8c46a6b00fc8f960736e9d2025-02-03T10:59:56ZengWileyAquaculture Nutrition1365-20952023-01-01202310.1155/2023/6676953Effect of Fish Meal Substitution with Black Soldier Fly (Hermetia illucens) on Growth Performance, Feed Stability, Blood Biochemistry, and Liver and Gut Morphology of Siamese Fighting Fish (Betta splendens)Zulhisyam Abdul Kari0Guillermo Téllez-Isaías1Noor Khalidah Abdul Hamid2Nor Dini Rusli3Khairiyah Mat4Suniza Anis Mohamad Sukri5Muhammad Anamul Kabir6Ahmad Razali Ishak7Nazri Che Dom8Abdel-Wahab A. Abdel-Warith9Elsayed M. Younis10Martina Irwan Khoo11Faizuan Abdullah12Md Shahjahan13Md Fazle Rohani14Simon J. Davies15Lee Seong Wei16Department of Agricultural SciencesDepartment of Poultry ScienceSchool of Biological SciencesDepartment of Agricultural SciencesDepartment of Agricultural SciencesDepartment of Agricultural SciencesAdvanced Livestock and Aquaculture Research GroupCentre for Environmental Health & Safety StudiesCentre for Environmental Health & Safety StudiesDepartment of ZoologyDepartment of ZoologyDepartment of Chemical PathologyDepartment of ChemistryLaboratory of Fish EcophysiologyDepartment of AquacultureAquaculture and Nutrition Research Unit (ANRU)Department of Agricultural SciencesInsects such as black soldier fly larvae (BSFL) are gaining interest among researchers and the aquafeed industry due to the fluctuating price and supply of fish meal (FM). This study evaluated the growth performance, feed stability, blood biochemistry, and liver and gut morphology of Betta splendens using BSFL as an alternative to FM. Five formulated diets were prepared: 0% BSFL, 6.5% BSFL, 13% BSFL, 19.5% BSFL, and 24.5% BSFL. The expansion rate, pellet durability index, floatability, bulk density, and water stability of the prepared feed have been assessed. Except for the diameter of the feed, all the parameters studied differed significantly (p<0.05) across the experimental diets. After 60 days, the fish fed with 13% BSFL had the highest final length, final weight, net weight gain, specific growth rate, weight gain, and gastrointestinal weight, with mean and standard deviation values of 3.97±0.43 cm, 3.95±0.1 g, 2.78±0.1 g, 4.63±0.17, 4.65±0.13, 237.26±7.9%, and 0.04±0.01 mg, respectively. Similar blood haematology and biochemical properties, including corpuscular volume, lymphocytes, white blood cells, red blood cells, haematocrit, albumin, and alkaline phosphatase, were the highest (p<0.05) in the 13% BSFL diet group compared to the other treatment groups. In addition, BSFL had a significant impact (p<0.05) on villus length, width, and crypt depth for the anterior and posterior guts of B. splendens. The 13% BSFL diet group had an intact epithelial barrier in the goblet cell arrangement and a well-organized villus structure and tunica muscularis, compared to the other treatment groups. Furthermore, the liver cell was altered with different BSFL inclusions; the 13% FM group demonstrated better nuclei and cytoplasm structure than the other treatment groups. In conclusion, replacing 13% FM with BSFL could improve the growth performance, blood parameters, and liver and intestine morphology of B. splendens, thus providing a promising alternative diet for ornamental freshwater fish.http://dx.doi.org/10.1155/2023/6676953 |
| spellingShingle | Zulhisyam Abdul Kari Guillermo Téllez-Isaías Noor Khalidah Abdul Hamid Nor Dini Rusli Khairiyah Mat Suniza Anis Mohamad Sukri Muhammad Anamul Kabir Ahmad Razali Ishak Nazri Che Dom Abdel-Wahab A. Abdel-Warith Elsayed M. Younis Martina Irwan Khoo Faizuan Abdullah Md Shahjahan Md Fazle Rohani Simon J. Davies Lee Seong Wei Effect of Fish Meal Substitution with Black Soldier Fly (Hermetia illucens) on Growth Performance, Feed Stability, Blood Biochemistry, and Liver and Gut Morphology of Siamese Fighting Fish (Betta splendens) Aquaculture Nutrition |
| title | Effect of Fish Meal Substitution with Black Soldier Fly (Hermetia illucens) on Growth Performance, Feed Stability, Blood Biochemistry, and Liver and Gut Morphology of Siamese Fighting Fish (Betta splendens) |
| title_full | Effect of Fish Meal Substitution with Black Soldier Fly (Hermetia illucens) on Growth Performance, Feed Stability, Blood Biochemistry, and Liver and Gut Morphology of Siamese Fighting Fish (Betta splendens) |
| title_fullStr | Effect of Fish Meal Substitution with Black Soldier Fly (Hermetia illucens) on Growth Performance, Feed Stability, Blood Biochemistry, and Liver and Gut Morphology of Siamese Fighting Fish (Betta splendens) |
| title_full_unstemmed | Effect of Fish Meal Substitution with Black Soldier Fly (Hermetia illucens) on Growth Performance, Feed Stability, Blood Biochemistry, and Liver and Gut Morphology of Siamese Fighting Fish (Betta splendens) |
| title_short | Effect of Fish Meal Substitution with Black Soldier Fly (Hermetia illucens) on Growth Performance, Feed Stability, Blood Biochemistry, and Liver and Gut Morphology of Siamese Fighting Fish (Betta splendens) |
| title_sort | effect of fish meal substitution with black soldier fly hermetia illucens on growth performance feed stability blood biochemistry and liver and gut morphology of siamese fighting fish betta splendens |
| url | http://dx.doi.org/10.1155/2023/6676953 |
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