Cell growth monitoring in a tetrapolar electrode configuration
There are various methods for cell growth monitoring. However, most of these methods have drawbacks, such as being invasive, not providing real-time results, or being costly. In this study, we present an alternate method of cell growth monitoring, which is low-cost, non-invasive, real-time, and uses...
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2024-07-01
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| Series: | Journal of Electrical Bioimpedance |
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| Online Access: | https://doi.org/10.2478/joeb-2024-0009 |
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| author | Singh Jagbir Khambete Niranjan D. |
| author_facet | Singh Jagbir Khambete Niranjan D. |
| author_sort | Singh Jagbir |
| collection | DOAJ |
| description | There are various methods for cell growth monitoring. However, most of these methods have drawbacks, such as being invasive, not providing real-time results, or being costly. In this study, we present an alternate method of cell growth monitoring, which is low-cost, non-invasive, real-time, and uses Electrical Impedance Spectro-scopy (EIS). In this work, commercially available culture plates were fitted with custom tetrapolar electrodes, and mouse cells were cultured on them. The variation of culture media impedance, resulting from cell growth, proliferation and other metabolic activities, was recorded over a period of seven days. The results demonstrated an initial increase in impedance corresponding with the cell growth phase, followed by a decrease during the cell death (apoptosis) phase, as confirmed by microscope images. Overall, the results show that our method to monitor cell growth using tetrapolar electrodes is promising and can be further refined for related applications. |
| format | Article |
| id | doaj-art-acd99e563072482b983c6479a4c0d8d1 |
| institution | Kabale University |
| issn | 1891-5469 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Electrical Bioimpedance |
| spelling | doaj-art-acd99e563072482b983c6479a4c0d8d12025-01-20T11:09:56ZengSciendoJournal of Electrical Bioimpedance1891-54692024-07-01151858810.2478/joeb-2024-0009Cell growth monitoring in a tetrapolar electrode configurationSingh Jagbir0Khambete Niranjan D.11Biotechnology, IIT Madras, Chennai, India2SCTIMST, Trivandrum, IndiaThere are various methods for cell growth monitoring. However, most of these methods have drawbacks, such as being invasive, not providing real-time results, or being costly. In this study, we present an alternate method of cell growth monitoring, which is low-cost, non-invasive, real-time, and uses Electrical Impedance Spectro-scopy (EIS). In this work, commercially available culture plates were fitted with custom tetrapolar electrodes, and mouse cells were cultured on them. The variation of culture media impedance, resulting from cell growth, proliferation and other metabolic activities, was recorded over a period of seven days. The results demonstrated an initial increase in impedance corresponding with the cell growth phase, followed by a decrease during the cell death (apoptosis) phase, as confirmed by microscope images. Overall, the results show that our method to monitor cell growth using tetrapolar electrodes is promising and can be further refined for related applications.https://doi.org/10.2478/joeb-2024-0009cell growth monitoringbioimpedanceelectrodeelectrode impedance spectroscopy |
| spellingShingle | Singh Jagbir Khambete Niranjan D. Cell growth monitoring in a tetrapolar electrode configuration Journal of Electrical Bioimpedance cell growth monitoring bioimpedance electrode electrode impedance spectroscopy |
| title | Cell growth monitoring in a tetrapolar electrode configuration |
| title_full | Cell growth monitoring in a tetrapolar electrode configuration |
| title_fullStr | Cell growth monitoring in a tetrapolar electrode configuration |
| title_full_unstemmed | Cell growth monitoring in a tetrapolar electrode configuration |
| title_short | Cell growth monitoring in a tetrapolar electrode configuration |
| title_sort | cell growth monitoring in a tetrapolar electrode configuration |
| topic | cell growth monitoring bioimpedance electrode electrode impedance spectroscopy |
| url | https://doi.org/10.2478/joeb-2024-0009 |
| work_keys_str_mv | AT singhjagbir cellgrowthmonitoringinatetrapolarelectrodeconfiguration AT khambeteniranjand cellgrowthmonitoringinatetrapolarelectrodeconfiguration |