Endophytic Fungi of <i>Calea pinnatifida</i> (Asteraceae): Dereplication of Crude Extracts, Antimicrobial Properties, and Identification of New Tetronic Acid Derivative Produced by <i>Hypomontagnella barbarensis</i>
Endophytic fungi are increasingly being recognized for their diverse metabolites that may exhibit antimicrobial properties. In our study, we isolated seven endophytic fungal strains from <i>Calea pinnatifida</i>, which were identified as <i>Hypomontagnella barbarensis</i>, &l...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/1/22 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588223966085120 |
|---|---|
| author | Bianca Barna Lhaís Araújo Caldas Jackson Monteiro Augusto Leonardo dos Santos Renata Castiglioni Pascon Marcelo Afonso Vallim Marcelo José Pena Ferreira Sarah Santos Gonçalves Glaucia Queiroz dos Santos Anderson Messias Rodrigues Jamile Ambrósio de Carvalho Suzan Pantaroto de Vasconcellos Patricia Sartorelli |
| author_facet | Bianca Barna Lhaís Araújo Caldas Jackson Monteiro Augusto Leonardo dos Santos Renata Castiglioni Pascon Marcelo Afonso Vallim Marcelo José Pena Ferreira Sarah Santos Gonçalves Glaucia Queiroz dos Santos Anderson Messias Rodrigues Jamile Ambrósio de Carvalho Suzan Pantaroto de Vasconcellos Patricia Sartorelli |
| author_sort | Bianca Barna |
| collection | DOAJ |
| description | Endophytic fungi are increasingly being recognized for their diverse metabolites that may exhibit antimicrobial properties. In our study, we isolated seven endophytic fungal strains from <i>Calea pinnatifida</i>, which were identified as <i>Hypomontagnella barbarensis</i>, <i>Neopestalotiopsis clavispora</i>, <i>Nigrospora sacchari-officinarum</i>, <i>Annulohypoxylon moriforme</i>, <i>Colletotrichum siamense</i>, and <i>Colletotrichum karstii</i> (with two isolates from the same species). Furthermore, the antimicrobial activity of the extracts was assessed, revealing that the extract from <i>Hypomontagnella barbarensis</i> demonstrated activity against <i>Staphylococcus aureus</i>. Further investigation of secondary metabolites, employing UHPLC-HR-ESI-MS/MS in combination with molecular networking, facilitated annotation of the nine compounds. Of these, five were identified based on matches with the GNPS spectral library, and four were predicted based on the molecular network. Notably, from the extract of <i>Hypomontagnella barbarensis</i>, two pairs of diastereoisomeric acyl-tetronic acid derivatives were isolated and characterized using MS and NMR spectroscopy. This study highlights the potential of endophytic fungi as a valuable source of novel antimicrobial agents. |
| format | Article |
| id | doaj-art-acd18ac90eb744a0a95254790b6ff404 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-acd18ac90eb744a0a95254790b6ff4042025-01-24T13:37:15ZengMDPI AGJournal of Fungi2309-608X2024-12-011112210.3390/jof11010022Endophytic Fungi of <i>Calea pinnatifida</i> (Asteraceae): Dereplication of Crude Extracts, Antimicrobial Properties, and Identification of New Tetronic Acid Derivative Produced by <i>Hypomontagnella barbarensis</i>Bianca Barna0Lhaís Araújo Caldas1Jackson Monteiro2Augusto Leonardo dos Santos3Renata Castiglioni Pascon4Marcelo Afonso Vallim5Marcelo José Pena Ferreira6Sarah Santos Gonçalves7Glaucia Queiroz dos Santos8Anderson Messias Rodrigues9Jamile Ambrósio de Carvalho10Suzan Pantaroto de Vasconcellos11Patricia Sartorelli12Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema 09972-270, SP, BrazilInstitute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema 09972-270, SP, BrazilInstitute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema 09972-270, SP, BrazilInstitute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema 09972-270, SP, BrazilInstitute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema 09972-270, SP, BrazilInstitute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema 09972-270, SP, BrazilDepartment of Botany, Institute of Biosciences, University of São Paulo, São Paulo 05508-090, SP, BrazilDepartment of Pathology, Federal University of Espírito Santo, Goiabeiras, Vitória 29075-910, ES, BrazilDepartment of Pathology, Federal University of Espírito Santo, Goiabeiras, Vitória 29075-910, ES, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04023-062, SP, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04023-062, SP, BrazilInstitute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema 09972-270, SP, BrazilInstitute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema 09972-270, SP, BrazilEndophytic fungi are increasingly being recognized for their diverse metabolites that may exhibit antimicrobial properties. In our study, we isolated seven endophytic fungal strains from <i>Calea pinnatifida</i>, which were identified as <i>Hypomontagnella barbarensis</i>, <i>Neopestalotiopsis clavispora</i>, <i>Nigrospora sacchari-officinarum</i>, <i>Annulohypoxylon moriforme</i>, <i>Colletotrichum siamense</i>, and <i>Colletotrichum karstii</i> (with two isolates from the same species). Furthermore, the antimicrobial activity of the extracts was assessed, revealing that the extract from <i>Hypomontagnella barbarensis</i> demonstrated activity against <i>Staphylococcus aureus</i>. Further investigation of secondary metabolites, employing UHPLC-HR-ESI-MS/MS in combination with molecular networking, facilitated annotation of the nine compounds. Of these, five were identified based on matches with the GNPS spectral library, and four were predicted based on the molecular network. Notably, from the extract of <i>Hypomontagnella barbarensis</i>, two pairs of diastereoisomeric acyl-tetronic acid derivatives were isolated and characterized using MS and NMR spectroscopy. This study highlights the potential of endophytic fungi as a valuable source of novel antimicrobial agents.https://www.mdpi.com/2309-608X/11/1/22<i>Calea pinnatifida</i>endophytic fungi<i>Hypomontagnella barbarensis</i>antimicrobial activitydereplication |
| spellingShingle | Bianca Barna Lhaís Araújo Caldas Jackson Monteiro Augusto Leonardo dos Santos Renata Castiglioni Pascon Marcelo Afonso Vallim Marcelo José Pena Ferreira Sarah Santos Gonçalves Glaucia Queiroz dos Santos Anderson Messias Rodrigues Jamile Ambrósio de Carvalho Suzan Pantaroto de Vasconcellos Patricia Sartorelli Endophytic Fungi of <i>Calea pinnatifida</i> (Asteraceae): Dereplication of Crude Extracts, Antimicrobial Properties, and Identification of New Tetronic Acid Derivative Produced by <i>Hypomontagnella barbarensis</i> Journal of Fungi <i>Calea pinnatifida</i> endophytic fungi <i>Hypomontagnella barbarensis</i> antimicrobial activity dereplication |
| title | Endophytic Fungi of <i>Calea pinnatifida</i> (Asteraceae): Dereplication of Crude Extracts, Antimicrobial Properties, and Identification of New Tetronic Acid Derivative Produced by <i>Hypomontagnella barbarensis</i> |
| title_full | Endophytic Fungi of <i>Calea pinnatifida</i> (Asteraceae): Dereplication of Crude Extracts, Antimicrobial Properties, and Identification of New Tetronic Acid Derivative Produced by <i>Hypomontagnella barbarensis</i> |
| title_fullStr | Endophytic Fungi of <i>Calea pinnatifida</i> (Asteraceae): Dereplication of Crude Extracts, Antimicrobial Properties, and Identification of New Tetronic Acid Derivative Produced by <i>Hypomontagnella barbarensis</i> |
| title_full_unstemmed | Endophytic Fungi of <i>Calea pinnatifida</i> (Asteraceae): Dereplication of Crude Extracts, Antimicrobial Properties, and Identification of New Tetronic Acid Derivative Produced by <i>Hypomontagnella barbarensis</i> |
| title_short | Endophytic Fungi of <i>Calea pinnatifida</i> (Asteraceae): Dereplication of Crude Extracts, Antimicrobial Properties, and Identification of New Tetronic Acid Derivative Produced by <i>Hypomontagnella barbarensis</i> |
| title_sort | endophytic fungi of i calea pinnatifida i asteraceae dereplication of crude extracts antimicrobial properties and identification of new tetronic acid derivative produced by i hypomontagnella barbarensis i |
| topic | <i>Calea pinnatifida</i> endophytic fungi <i>Hypomontagnella barbarensis</i> antimicrobial activity dereplication |
| url | https://www.mdpi.com/2309-608X/11/1/22 |
| work_keys_str_mv | AT biancabarna endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT lhaisaraujocaldas endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT jacksonmonteiro endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT augustoleonardodossantos endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT renatacastiglionipascon endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT marceloafonsovallim endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT marcelojosepenaferreira endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT sarahsantosgoncalves endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT glauciaqueirozdossantos endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT andersonmessiasrodrigues endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT jamileambrosiodecarvalho endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT suzanpantarotodevasconcellos endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi AT patriciasartorelli endophyticfungioficaleapinnatifidaiasteraceaedereplicationofcrudeextractsantimicrobialpropertiesandidentificationofnewtetronicacidderivativeproducedbyihypomontagnellabarbarensisi |