Enhanced Production of Cellulase from Pineapple Waste by Response Surface Methodology
Optimization of the media components for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using pineapple waste as substrate was performed with statistical methodology based on experimental designs. The screening of nutrients and their influence...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Engineering |
| Online Access: | http://dx.doi.org/10.1155/2013/979547 |
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| author | P. Saravanan R. Muthuvelayudham T. Viruthagiri |
| author_facet | P. Saravanan R. Muthuvelayudham T. Viruthagiri |
| author_sort | P. Saravanan |
| collection | DOAJ |
| description | Optimization of the media components for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using pineapple waste as substrate was performed with statistical methodology based on experimental designs. The screening of nutrients and their influence on the cellulase production was studied using a Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and yeast extract were found to have the positive influence for the production of cellulase. The selected components were optimized using response surface methodology. The optimum concentrations are avicel: 26.5 g/L, soybean cake flour: 22.5 g/L, KH2PO4: 4.5 g/L, and yeast extract: 12.3 g/L. A maximum cellulase activity of 8.61 IU/mL was obtained under the optimized medium in the validation experiment. |
| format | Article |
| id | doaj-art-accf289936564e41996c8e0ce6466fdd |
| institution | Kabale University |
| issn | 2314-4904 2314-4912 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Engineering |
| spelling | doaj-art-accf289936564e41996c8e0ce6466fdd2025-02-03T01:03:02ZengWileyJournal of Engineering2314-49042314-49122013-01-01201310.1155/2013/979547979547Enhanced Production of Cellulase from Pineapple Waste by Response Surface MethodologyP. Saravanan0R. Muthuvelayudham1T. Viruthagiri2Department of Chemical Engineering, Annamalai University, Annamalainagar 608002, Tamilnadu, IndiaDepartment of Chemical Engineering, Annamalai University, Annamalainagar 608002, Tamilnadu, IndiaDepartment of Chemical Engineering, Annamalai University, Annamalainagar 608002, Tamilnadu, IndiaOptimization of the media components for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using pineapple waste as substrate was performed with statistical methodology based on experimental designs. The screening of nutrients and their influence on the cellulase production was studied using a Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and yeast extract were found to have the positive influence for the production of cellulase. The selected components were optimized using response surface methodology. The optimum concentrations are avicel: 26.5 g/L, soybean cake flour: 22.5 g/L, KH2PO4: 4.5 g/L, and yeast extract: 12.3 g/L. A maximum cellulase activity of 8.61 IU/mL was obtained under the optimized medium in the validation experiment.http://dx.doi.org/10.1155/2013/979547 |
| spellingShingle | P. Saravanan R. Muthuvelayudham T. Viruthagiri Enhanced Production of Cellulase from Pineapple Waste by Response Surface Methodology Journal of Engineering |
| title | Enhanced Production of Cellulase from Pineapple Waste by Response Surface Methodology |
| title_full | Enhanced Production of Cellulase from Pineapple Waste by Response Surface Methodology |
| title_fullStr | Enhanced Production of Cellulase from Pineapple Waste by Response Surface Methodology |
| title_full_unstemmed | Enhanced Production of Cellulase from Pineapple Waste by Response Surface Methodology |
| title_short | Enhanced Production of Cellulase from Pineapple Waste by Response Surface Methodology |
| title_sort | enhanced production of cellulase from pineapple waste by response surface methodology |
| url | http://dx.doi.org/10.1155/2013/979547 |
| work_keys_str_mv | AT psaravanan enhancedproductionofcellulasefrompineapplewastebyresponsesurfacemethodology AT rmuthuvelayudham enhancedproductionofcellulasefrompineapplewastebyresponsesurfacemethodology AT tviruthagiri enhancedproductionofcellulasefrompineapplewastebyresponsesurfacemethodology |