α-spinasterol isolated from Achyranthes aspera L. ameliorates inflammation via NF-κB and Nrf2/HO-1 pathways
Abstract On the basis of previous studies, the low-polar part of Achyranthes aspera L. (A. aspera) had strong anti-inflammatory activity. Three compounds were isolated from the low polarity fraction of A. aspera, and their structures were identified as α-spinasterol (1), 7,8-dihydrospinasterol (2),...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-90022-2 |
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| Summary: | Abstract On the basis of previous studies, the low-polar part of Achyranthes aspera L. (A. aspera) had strong anti-inflammatory activity. Three compounds were isolated from the low polarity fraction of A. aspera, and their structures were identified as α-spinasterol (1), 7,8-dihydrospinasterol (2), 22,23-dihydrospinasterol (3). Among them, the content of α-spinasterol (1) in A. aspera was higher in the spring and winter seasons through HPLC methods, ranging from 0.0085 to 0.0157%. Futhermore, in the LPS-induced RAW264.7 cells inflammation model, α-spinasterol significantly reduced the levels of cytokines such as IL-6, PGE2 and TNF-α, inhibited the expression of COX-2, 5-LOX, p-IKKβ, p-NFκB and p-IkBα proteins, and promoted the expression of Nrf2, HO-1 and NQO1 proteins. Therefore, this study showed that α-spinasterol can inhibit LPS-induced RAW264.7 cells inflammation, and its mechanism may be related to the inhibition of NF-κB pathway, activation of Nrf2 pathway, and reduction of excessive release of inflammatory factors. |
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| ISSN: | 2045-2322 |