Genome-Wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt Stress
Various CBL-interacting protein kinases (CIPKs) are involved in abiotic stress responses in plants. Despite the economic importance of jasmine (<i>Jasminum sambac</i> L. Aiton) and the availability of genome data, there are few reports analyzing the CIPK gene family. In this study, genom...
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2025-01-01
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| author | Shuang Zhang Xin Huang Lili Yin Jiawei Li Jiacan Xu Ruigang Wu |
| author_facet | Shuang Zhang Xin Huang Lili Yin Jiawei Li Jiacan Xu Ruigang Wu |
| author_sort | Shuang Zhang |
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| description | Various CBL-interacting protein kinases (CIPKs) are involved in abiotic stress responses in plants. Despite the economic importance of jasmine (<i>Jasminum sambac</i> L. Aiton) and the availability of genome data, there are few reports analyzing the CIPK gene family. In this study, genome-wide identification of the CIPK gene family in jasmine was conducted, which would provide valuable information for the function analysis of <i>JsCIPKs</i> regarding participation in growth and development and response to salt stress. In the present study, a total of 17 <i>CIPKs</i> were identified, which were unevenly distributed on eight chromosomes. The JsCIPK protein sequences contained 311–781 amino acids, with a molecular weight of 35.05–87.58 kDa. Phylogenetic analysis revealed that the 17 JsCIPKs could be divided into five classical branches. <i>JsCIPK</i> genes with higher homology showed greater similarity between conserved protein motifs. Collinearity analysis demonstrated that 13 gene pairs in Arabidopsis were collinear with the jasmine sequences. Various hormone-related response- and stress-induced elements were observed in the promoter region of <i>JsCIPK</i> genes, such as TC-rich repeats, CARE, etc. Furthermore, the expression of <i>JsCIPK</i> genes varied in different organs. Finally, the expression analyses of eight <i>JsCIPKs</i> under salt stress were performed. A systematic analysis of the CIPK gene family and the effect of salt stress on the expression of eight <i>JsCIPK</i> genes in leaves of jasmine was carried out. The expression of <i>JsCIPK6</i> and <i>JsCIPK8</i> was significantly down-regulated and up-regulated by salt treatment, respectively. These findings would lay a foundation for future functional studies of these two genes in jasmine related to salt stress and provide useful resistance genes for the molecular breeding of new varieties of salt-tolerant jasmine. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Horticulturae |
| spelling | doaj-art-ac33c71f1846450eb88faf96f4c3e06a2025-01-24T13:34:33ZengMDPI AGHorticulturae2311-75242025-01-011114010.3390/horticulturae11010040Genome-Wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt StressShuang Zhang0Xin Huang1Lili Yin2Jiawei Li3Jiacan Xu4Ruigang Wu5School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, ChinaCollege of Horticulture, China Agricultural University, Beijing 100091, ChinaCollege of Agronomy and Life Sciences, Shanxi Datong University, Datong 037009, ChinaSchool of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, ChinaSchool of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, ChinaSchool of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, ChinaVarious CBL-interacting protein kinases (CIPKs) are involved in abiotic stress responses in plants. Despite the economic importance of jasmine (<i>Jasminum sambac</i> L. Aiton) and the availability of genome data, there are few reports analyzing the CIPK gene family. In this study, genome-wide identification of the CIPK gene family in jasmine was conducted, which would provide valuable information for the function analysis of <i>JsCIPKs</i> regarding participation in growth and development and response to salt stress. In the present study, a total of 17 <i>CIPKs</i> were identified, which were unevenly distributed on eight chromosomes. The JsCIPK protein sequences contained 311–781 amino acids, with a molecular weight of 35.05–87.58 kDa. Phylogenetic analysis revealed that the 17 JsCIPKs could be divided into five classical branches. <i>JsCIPK</i> genes with higher homology showed greater similarity between conserved protein motifs. Collinearity analysis demonstrated that 13 gene pairs in Arabidopsis were collinear with the jasmine sequences. Various hormone-related response- and stress-induced elements were observed in the promoter region of <i>JsCIPK</i> genes, such as TC-rich repeats, CARE, etc. Furthermore, the expression of <i>JsCIPK</i> genes varied in different organs. Finally, the expression analyses of eight <i>JsCIPKs</i> under salt stress were performed. A systematic analysis of the CIPK gene family and the effect of salt stress on the expression of eight <i>JsCIPK</i> genes in leaves of jasmine was carried out. The expression of <i>JsCIPK6</i> and <i>JsCIPK8</i> was significantly down-regulated and up-regulated by salt treatment, respectively. These findings would lay a foundation for future functional studies of these two genes in jasmine related to salt stress and provide useful resistance genes for the molecular breeding of new varieties of salt-tolerant jasmine.https://www.mdpi.com/2311-7524/11/1/40<i>Jasminum sambac</i>bioinformatics analysisphylogenetic relationshipsgene structurecollinearity analysis |
| spellingShingle | Shuang Zhang Xin Huang Lili Yin Jiawei Li Jiacan Xu Ruigang Wu Genome-Wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt Stress Horticulturae <i>Jasminum sambac</i> bioinformatics analysis phylogenetic relationships gene structure collinearity analysis |
| title | Genome-Wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt Stress |
| title_full | Genome-Wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt Stress |
| title_fullStr | Genome-Wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt Stress |
| title_full_unstemmed | Genome-Wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt Stress |
| title_short | Genome-Wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt Stress |
| title_sort | genome wide identification of the cipk gene family in jasmine and expression analysis under salt stress |
| topic | <i>Jasminum sambac</i> bioinformatics analysis phylogenetic relationships gene structure collinearity analysis |
| url | https://www.mdpi.com/2311-7524/11/1/40 |
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