Effects of dietary supplements Sargassum ilicifolium and Spirulina platensis on growth parameters, immunity and gene expression in juvenile Asian seabass (Lates calcarifer)
Background: Microalgae and macroalgae play significant roles in aquaculture, offering various benefits that contribute to sustainable practices in the industry. Methods: The aim of this research was to investigate the impact of feeding Lates calcarifer a combination of Sargassum ilicifolium and Spir...
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Elsevier
2025-03-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154325000602 |
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| author | Majid Khanzadeh Seyed Hossein Hoseinifar Ashkan Zargari Hien Van Doan |
| author_facet | Majid Khanzadeh Seyed Hossein Hoseinifar Ashkan Zargari Hien Van Doan |
| author_sort | Majid Khanzadeh |
| collection | DOAJ |
| description | Background: Microalgae and macroalgae play significant roles in aquaculture, offering various benefits that contribute to sustainable practices in the industry. Methods: The aim of this research was to investigate the impact of feeding Lates calcarifer a combination of Sargassum ilicifolium and Spirulina platensis on growth, immune responses, and the expression of TNF-α, IL-1β, and IGF-1 genes. Six hundred juvenile Asian sea bass, weighing about 50 ± 2 g, were divided into five groups: a control group (CTRL) and groups receiving varying percentages of Sargassum ilicifolium and Spirulina platensis (1.5 %, 2 %, 2.5 %, and 3 %). Results: The results showed that adding 2.5 % combined algal supplement (CAS) to the diet of Asian seabass increased the activity of digestive enzymes such as lipase, trypsin, and α-amylase in the gastrointestinal tract. Also, a significant increase (P < 0.05) in weight gain (WG) and specific growth rate (SGR) and a significant decrease in the feed conversion ratio (FCR) were noticed in CAS-fed fish. The highest levels of skin mucus lysozyme activity and total Ig levels were observed in the group that received 2.5 % CAS (P < 0.05). Furthermore, this group also showed the highest levels of complement C3, respiratory burst activity, and total Ig in the serum (P < 0.05). At the molecular level, using 2.5 % dietary CAS was associated with a significant increase in the expression of TNF-α, IL-1β, and IGF-1 genes compared to the control group (P < 0.05). The study also demonstrated that feeding Asian seabass with 2.5 % CAS resulted in a significant increase in the survival rate of juvenile fish exposed to Vibrio harveyi. Conclusions: These results showed that using combined S. ilicifolium and S. platensis can be considered a beneficial feed additive for Asian seabass aquaculture. |
| format | Article |
| id | doaj-art-ac3023226664492d8ec14abd61563569 |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-ac3023226664492d8ec14abd615635692025-02-04T04:10:35ZengElsevierJournal of Agriculture and Food Research2666-15432025-03-0119101689Effects of dietary supplements Sargassum ilicifolium and Spirulina platensis on growth parameters, immunity and gene expression in juvenile Asian seabass (Lates calcarifer)Majid Khanzadeh0Seyed Hossein Hoseinifar1Ashkan Zargari2Hien Van Doan3Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran; Animal Biological Product Research Group, Academic Center for Education, Culture and Research (ACECR), Tehran Organization, Tehran, Iran; Corresponding author. Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, IranDepartment of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, IranDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand; Functional Feed Innovation Center (FuncFeed), Faculty of Agriculture, Chiang Mai University, Chiang Mai, ThailandBackground: Microalgae and macroalgae play significant roles in aquaculture, offering various benefits that contribute to sustainable practices in the industry. Methods: The aim of this research was to investigate the impact of feeding Lates calcarifer a combination of Sargassum ilicifolium and Spirulina platensis on growth, immune responses, and the expression of TNF-α, IL-1β, and IGF-1 genes. Six hundred juvenile Asian sea bass, weighing about 50 ± 2 g, were divided into five groups: a control group (CTRL) and groups receiving varying percentages of Sargassum ilicifolium and Spirulina platensis (1.5 %, 2 %, 2.5 %, and 3 %). Results: The results showed that adding 2.5 % combined algal supplement (CAS) to the diet of Asian seabass increased the activity of digestive enzymes such as lipase, trypsin, and α-amylase in the gastrointestinal tract. Also, a significant increase (P < 0.05) in weight gain (WG) and specific growth rate (SGR) and a significant decrease in the feed conversion ratio (FCR) were noticed in CAS-fed fish. The highest levels of skin mucus lysozyme activity and total Ig levels were observed in the group that received 2.5 % CAS (P < 0.05). Furthermore, this group also showed the highest levels of complement C3, respiratory burst activity, and total Ig in the serum (P < 0.05). At the molecular level, using 2.5 % dietary CAS was associated with a significant increase in the expression of TNF-α, IL-1β, and IGF-1 genes compared to the control group (P < 0.05). The study also demonstrated that feeding Asian seabass with 2.5 % CAS resulted in a significant increase in the survival rate of juvenile fish exposed to Vibrio harveyi. Conclusions: These results showed that using combined S. ilicifolium and S. platensis can be considered a beneficial feed additive for Asian seabass aquaculture.http://www.sciencedirect.com/science/article/pii/S2666154325000602S.ilicifoliumS. platensisGrowthGene expressionV. harveyiAsian seabass |
| spellingShingle | Majid Khanzadeh Seyed Hossein Hoseinifar Ashkan Zargari Hien Van Doan Effects of dietary supplements Sargassum ilicifolium and Spirulina platensis on growth parameters, immunity and gene expression in juvenile Asian seabass (Lates calcarifer) Journal of Agriculture and Food Research S.ilicifolium S. platensis Growth Gene expression V. harveyi Asian seabass |
| title | Effects of dietary supplements Sargassum ilicifolium and Spirulina platensis on growth parameters, immunity and gene expression in juvenile Asian seabass (Lates calcarifer) |
| title_full | Effects of dietary supplements Sargassum ilicifolium and Spirulina platensis on growth parameters, immunity and gene expression in juvenile Asian seabass (Lates calcarifer) |
| title_fullStr | Effects of dietary supplements Sargassum ilicifolium and Spirulina platensis on growth parameters, immunity and gene expression in juvenile Asian seabass (Lates calcarifer) |
| title_full_unstemmed | Effects of dietary supplements Sargassum ilicifolium and Spirulina platensis on growth parameters, immunity and gene expression in juvenile Asian seabass (Lates calcarifer) |
| title_short | Effects of dietary supplements Sargassum ilicifolium and Spirulina platensis on growth parameters, immunity and gene expression in juvenile Asian seabass (Lates calcarifer) |
| title_sort | effects of dietary supplements sargassum ilicifolium and spirulina platensis on growth parameters immunity and gene expression in juvenile asian seabass lates calcarifer |
| topic | S.ilicifolium S. platensis Growth Gene expression V. harveyi Asian seabass |
| url | http://www.sciencedirect.com/science/article/pii/S2666154325000602 |
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