The Effects of Microseconds Electroporation on Pore Size, Viability and Mitochondrial Membrane Potential of Cervical Cancer Cells
Objective: Electroporation (EP) is a method in which the membrane permeability is increased by applying electrical pulses. The determination of modifications that occur in cells subsequent to EP with varying pulse parameters holds significant importance in establishing the foundations of EP theory....
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Galenos Publishing House
2025-01-01
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| Online Access: | https://www.bezmialemscience.org/articles/the-effects-of-microseconds-electroporation-on-pore-size-viability-and-mitochondrial-membrane-potential-of-cervical-cancer-cells/doi/bas.galenos.2024.40374 |
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| author | Güney GÜRSOY Meriç Arda ESMEKAYA Zehra ÇİÇEK |
| author_facet | Güney GÜRSOY Meriç Arda ESMEKAYA Zehra ÇİÇEK |
| author_sort | Güney GÜRSOY |
| collection | DOAJ |
| description | Objective: Electroporation (EP) is a method in which the membrane permeability is increased by applying electrical pulses. The determination of modifications that occur in cells subsequent to EP with varying pulse parameters holds significant importance in establishing the foundations of EP theory. Therefore, we sought clarification regarding the phenomenon of pore formation on the membrane of the electroporated human cervical cancer cell line (HeLa) cells.
Methods: The pores created on the cell membrane due to EP was observed using a scanning electron microscope. The change in the viability and mitochondrial membrane potential (ΔΨm) of cells was determined by WST-8 and JC-1 assays.
Results: The surface of the electroporated cell membrane exhibited a relatively uniform pore population. The viability of HeLa cells was significantly reduced with increasing electric field intensities. A slight decrease in ΔΨm was observed between the control and the 0.8 and 1.6 kV/cm EP groups, but ΔΨm was higher in the 2.4 and 3.2 kV/cm EP groups compared to the control group.
Conclusion: In conclusion, our study showed that the application of EP to the cervical cancer cell line resulted in the formation of pores of varying sizes on the membrane. While cell viability decreased with increasing electric field amplitude, no significant change was observed in ΔΨm between EP treatment and control groups. It should be noted that further research is needed to determine the pore distributions in electroporated cells and the resulting changes at different electric field amplitudes. |
| format | Article |
| id | doaj-art-ac0778545ab04334b34c2c8ded98db37 |
| institution | Kabale University |
| issn | 2148-2373 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Bezmiâlem Science |
| spelling | doaj-art-ac0778545ab04334b34c2c8ded98db372025-01-27T12:38:35ZengGalenos Publishing HouseBezmiâlem Science2148-23732025-01-01131586410.14235/bas.galenos.2024.40374The Effects of Microseconds Electroporation on Pore Size, Viability and Mitochondrial Membrane Potential of Cervical Cancer CellsGüney GÜRSOY0https://orcid.org/0000-0003-1849-9068Meriç Arda ESMEKAYA1https://orcid.org/0000-0003-0469-4954Zehra ÇİÇEK2https://orcid.org/0000-0003-3205-5463Kırşehir Ahi Evran University Faculty of Medicine, Department of Biophysics, Kırşehir, TürkiyeGazi University Faculty of Medicine, Department of Biophysics, Ankara, TürkiyeUniversity of Health Sciences Türkiye, Gülhane Faculty of Medicine, Department of Physiology, Ankara, TürkiyeObjective: Electroporation (EP) is a method in which the membrane permeability is increased by applying electrical pulses. The determination of modifications that occur in cells subsequent to EP with varying pulse parameters holds significant importance in establishing the foundations of EP theory. Therefore, we sought clarification regarding the phenomenon of pore formation on the membrane of the electroporated human cervical cancer cell line (HeLa) cells. Methods: The pores created on the cell membrane due to EP was observed using a scanning electron microscope. The change in the viability and mitochondrial membrane potential (ΔΨm) of cells was determined by WST-8 and JC-1 assays. Results: The surface of the electroporated cell membrane exhibited a relatively uniform pore population. The viability of HeLa cells was significantly reduced with increasing electric field intensities. A slight decrease in ΔΨm was observed between the control and the 0.8 and 1.6 kV/cm EP groups, but ΔΨm was higher in the 2.4 and 3.2 kV/cm EP groups compared to the control group. Conclusion: In conclusion, our study showed that the application of EP to the cervical cancer cell line resulted in the formation of pores of varying sizes on the membrane. While cell viability decreased with increasing electric field amplitude, no significant change was observed in ΔΨm between EP treatment and control groups. It should be noted that further research is needed to determine the pore distributions in electroporated cells and the resulting changes at different electric field amplitudes.https://www.bezmialemscience.org/articles/the-effects-of-microseconds-electroporation-on-pore-size-viability-and-mitochondrial-membrane-potential-of-cervical-cancer-cells/doi/bas.galenos.2024.40374electroporationcervical cancerpore sizecell viabilitymitochondrial membrane potential |
| spellingShingle | Güney GÜRSOY Meriç Arda ESMEKAYA Zehra ÇİÇEK The Effects of Microseconds Electroporation on Pore Size, Viability and Mitochondrial Membrane Potential of Cervical Cancer Cells Bezmiâlem Science electroporation cervical cancer pore size cell viability mitochondrial membrane potential |
| title | The Effects of Microseconds Electroporation on Pore Size, Viability and Mitochondrial Membrane Potential of Cervical Cancer Cells |
| title_full | The Effects of Microseconds Electroporation on Pore Size, Viability and Mitochondrial Membrane Potential of Cervical Cancer Cells |
| title_fullStr | The Effects of Microseconds Electroporation on Pore Size, Viability and Mitochondrial Membrane Potential of Cervical Cancer Cells |
| title_full_unstemmed | The Effects of Microseconds Electroporation on Pore Size, Viability and Mitochondrial Membrane Potential of Cervical Cancer Cells |
| title_short | The Effects of Microseconds Electroporation on Pore Size, Viability and Mitochondrial Membrane Potential of Cervical Cancer Cells |
| title_sort | effects of microseconds electroporation on pore size viability and mitochondrial membrane potential of cervical cancer cells |
| topic | electroporation cervical cancer pore size cell viability mitochondrial membrane potential |
| url | https://www.bezmialemscience.org/articles/the-effects-of-microseconds-electroporation-on-pore-size-viability-and-mitochondrial-membrane-potential-of-cervical-cancer-cells/doi/bas.galenos.2024.40374 |
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