Microbiota-derived β carotene is required for strobilation of Aurelia aurita by impacting host retinoic acid signaling
Summary: The strobilation process, an asexual reproduction mechanism in Aurelia aurita, transitions from the sessile polyp to the pelagic medusa stage. This study explored the essential role of the microbiome in strobilation, particularly through bacterial beta carotene’s impact on the host’s retino...
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| Language: | English |
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Elsevier
2025-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224029560 |
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| author | Nadin Jensen Nancy Weiland-Bräuer Cynthia Maria Chibani Ruth Anne Schmitz |
| author_facet | Nadin Jensen Nancy Weiland-Bräuer Cynthia Maria Chibani Ruth Anne Schmitz |
| author_sort | Nadin Jensen |
| collection | DOAJ |
| description | Summary: The strobilation process, an asexual reproduction mechanism in Aurelia aurita, transitions from the sessile polyp to the pelagic medusa stage. This study explored the essential role of the microbiome in strobilation, particularly through bacterial beta carotene’s impact on the host’s retinoic acid signaling pathway. Experiments demonstrated that native polyps undergo normal strobilation while sterile polyps exhibit morphological defects. Supplementing sterile polyps with provitamin A beta carotene or the vitamin A metabolite 9-cis retinoic acid (RA) remedied these defects, underscoring their crucial role in strobilation. Transcriptional analysis revealed that beta carotene and 9-cis RA restored expression of strobilation genes in sterile polyps to native levels. Inhibition of key enzymes in the RA pathway disrupted strobilation, further confirming its importance. The expression of bacterial β-carotenoid synthesis genes in the native microbiome, contrasted with tremendously reduced expression in antibiotic-treated polyps, emphasizes the microbiome’s pivotal role in beta carotene provision, facilitating A. aurita’s strobilation through RA signaling. |
| format | Article |
| id | doaj-art-abfff13dc1ff495098b160a6963fe50c |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-abfff13dc1ff495098b160a6963fe50c2025-02-05T04:32:31ZengElsevieriScience2589-00422025-02-01282111729Microbiota-derived β carotene is required for strobilation of Aurelia aurita by impacting host retinoic acid signalingNadin Jensen0Nancy Weiland-Bräuer1Cynthia Maria Chibani2Ruth Anne Schmitz3Institute of General Microbiology, Christian-Albrechts University Kiel, Am Botanischen Garten 1-9, 24118 Kiel, GermanyInstitute of General Microbiology, Christian-Albrechts University Kiel, Am Botanischen Garten 1-9, 24118 Kiel, GermanyInstitute of General Microbiology, Christian-Albrechts University Kiel, Am Botanischen Garten 1-9, 24118 Kiel, GermanyInstitute of General Microbiology, Christian-Albrechts University Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany; Corresponding authorSummary: The strobilation process, an asexual reproduction mechanism in Aurelia aurita, transitions from the sessile polyp to the pelagic medusa stage. This study explored the essential role of the microbiome in strobilation, particularly through bacterial beta carotene’s impact on the host’s retinoic acid signaling pathway. Experiments demonstrated that native polyps undergo normal strobilation while sterile polyps exhibit morphological defects. Supplementing sterile polyps with provitamin A beta carotene or the vitamin A metabolite 9-cis retinoic acid (RA) remedied these defects, underscoring their crucial role in strobilation. Transcriptional analysis revealed that beta carotene and 9-cis RA restored expression of strobilation genes in sterile polyps to native levels. Inhibition of key enzymes in the RA pathway disrupted strobilation, further confirming its importance. The expression of bacterial β-carotenoid synthesis genes in the native microbiome, contrasted with tremendously reduced expression in antibiotic-treated polyps, emphasizes the microbiome’s pivotal role in beta carotene provision, facilitating A. aurita’s strobilation through RA signaling.http://www.sciencedirect.com/science/article/pii/S2589004224029560BiochemistryCell biologyDevelopmental biologyMicrobiology |
| spellingShingle | Nadin Jensen Nancy Weiland-Bräuer Cynthia Maria Chibani Ruth Anne Schmitz Microbiota-derived β carotene is required for strobilation of Aurelia aurita by impacting host retinoic acid signaling iScience Biochemistry Cell biology Developmental biology Microbiology |
| title | Microbiota-derived β carotene is required for strobilation of Aurelia aurita by impacting host retinoic acid signaling |
| title_full | Microbiota-derived β carotene is required for strobilation of Aurelia aurita by impacting host retinoic acid signaling |
| title_fullStr | Microbiota-derived β carotene is required for strobilation of Aurelia aurita by impacting host retinoic acid signaling |
| title_full_unstemmed | Microbiota-derived β carotene is required for strobilation of Aurelia aurita by impacting host retinoic acid signaling |
| title_short | Microbiota-derived β carotene is required for strobilation of Aurelia aurita by impacting host retinoic acid signaling |
| title_sort | microbiota derived β carotene is required for strobilation of aurelia aurita by impacting host retinoic acid signaling |
| topic | Biochemistry Cell biology Developmental biology Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224029560 |
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